Exogenous Nitric Oxide Donation During In Vitro Maturation Improves Embryonic Development after Parthenogenesis and Somatic Cell Nuclear Transfer in Pigs

Nitric oxide (NO) has an important role in oocyte maturation and embryonic development in mammals. This study examined the effect of exogenous NO donor S-nitroso-N-acetylpenicillamine (SNAP) in a maturation medium on meiotic progression and embryonic development after parthenogenesis (PA) and somati...

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Main Authors: Fazle Elahi, Hyeji Shin, Joohyeong Lee, Seung Tae Lee, Geun-Shik Lee, Eunsong Lee
Format: Article
Language:English
Published: The Korean Society of Animal Reproduction and Biotechnology 2018-12-01
Series:Journal of Animal Reproduction and Biotechnology
Subjects:
Online Access:http://www.e-jarb.org/journal/view.html?uid=68&vmd=Full
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author Fazle Elahi
Hyeji Shin
Joohyeong Lee
Seung Tae Lee
Geun-Shik Lee
Eunsong Lee
author_facet Fazle Elahi
Hyeji Shin
Joohyeong Lee
Seung Tae Lee
Geun-Shik Lee
Eunsong Lee
author_sort Fazle Elahi
collection DOAJ
description Nitric oxide (NO) has an important role in oocyte maturation and embryonic development in mammals. This study examined the effect of exogenous NO donor S-nitroso-N-acetylpenicillamine (SNAP) in a maturation medium on meiotic progression and embryonic development after parthenogenesis (PA) and somatic cell nuclear transfer (SCNT) in pigs. When oocytes were exposed to 0.1 μM SNAP for first 22 h of in vitro maturation (IVM) in Experiment 1, SNAP significantly improved blastocyst development in both defined and standard follicular fluid-supplemented media compared to untreated control (48.4 vs. 31.7-42.5%). SNAP treatment significantly arrested meiotic progression of oocytes at the germinal vesicle stage at 11 h of IVM (61.2 vs. 38.7%). However, there was no effect on meiotic progression at 22 h of IVM (Experiment 2). In Experiment 3, when oocytes were treated with SNAP at 0.001, 0.1 and 10 μM during the first 22 h of IVM to determine a suitable concentration, 0.1 μM SNAP (54.2%) exhibited a higher blastocyst formation than 0 and 10 μM SNAP (36.6 and 36.6%, respectively). Time-dependent effect of SNAP treatment was evaluated in Experiment 4. It was observed that SNAP treatment for the first 22 h of IVM significantly increased blastocyst formation compared to no treatment (57.1% vs. 46.2%). Antioxidant effect of SNAP was compared with that of cysteine. SNAP treatment significantly improved embryonic development to the blastocyst stage (49.1-51.5% vs. 34.4-37.5%) irrespective of the presence or absence of cysteine (Experiment 5). Moreover, SNAP significantly increased glutathione (GSH) content and inversely decreased the reactive oxygen species (ROS) level and mitochondrial oxidative activity in IVM oocytes. SNAP treatment during IVM showed a stimulating effect on in vitro development of SCNT embryos (Experiment 7). These results demonstrates that SNAP improves developmental competence of PA and SCNT embryos probably by maintaining the redox homeostasis through increasing GSH content and mitochondrial quality and decreasing ROS in IVM oocytes
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spelling doaj.art-a39708c515014a3f81715e679c27e8452022-12-21T20:21:55ZengThe Korean Society of Animal Reproduction and BiotechnologyJournal of Animal Reproduction and Biotechnology2671-46392671-46632018-12-0133421122010.12750/JET.2018.33.4.211Exogenous Nitric Oxide Donation During In Vitro Maturation Improves Embryonic Development after Parthenogenesis and Somatic Cell Nuclear Transfer in PigsFazle Elahi0Hyeji Shin1Joohyeong Lee2Seung Tae Lee3Geun-Shik Lee4Eunsong Lee5College of Veterinary Medicine, Kangwon National University, Gangwon 24341, KoreaCollege of Veterinary Medicine, Kangwon National University, Gangwon 24341, KoreaInstitute of Veterinary Science, Kangwon National University, Gangwon 24341, KoreaDivision of Applied Animal Science, College of Animal Life Science, Kangwon National University, Gangwon 24341, KoreaCollege of Veterinary Medicine, Kangwon National University, Gangwon 24341, KoreCollege of Veterinary Medicine, Kangwon National University, Gangwon 24341, KoreaNitric oxide (NO) has an important role in oocyte maturation and embryonic development in mammals. This study examined the effect of exogenous NO donor S-nitroso-N-acetylpenicillamine (SNAP) in a maturation medium on meiotic progression and embryonic development after parthenogenesis (PA) and somatic cell nuclear transfer (SCNT) in pigs. When oocytes were exposed to 0.1 μM SNAP for first 22 h of in vitro maturation (IVM) in Experiment 1, SNAP significantly improved blastocyst development in both defined and standard follicular fluid-supplemented media compared to untreated control (48.4 vs. 31.7-42.5%). SNAP treatment significantly arrested meiotic progression of oocytes at the germinal vesicle stage at 11 h of IVM (61.2 vs. 38.7%). However, there was no effect on meiotic progression at 22 h of IVM (Experiment 2). In Experiment 3, when oocytes were treated with SNAP at 0.001, 0.1 and 10 μM during the first 22 h of IVM to determine a suitable concentration, 0.1 μM SNAP (54.2%) exhibited a higher blastocyst formation than 0 and 10 μM SNAP (36.6 and 36.6%, respectively). Time-dependent effect of SNAP treatment was evaluated in Experiment 4. It was observed that SNAP treatment for the first 22 h of IVM significantly increased blastocyst formation compared to no treatment (57.1% vs. 46.2%). Antioxidant effect of SNAP was compared with that of cysteine. SNAP treatment significantly improved embryonic development to the blastocyst stage (49.1-51.5% vs. 34.4-37.5%) irrespective of the presence or absence of cysteine (Experiment 5). Moreover, SNAP significantly increased glutathione (GSH) content and inversely decreased the reactive oxygen species (ROS) level and mitochondrial oxidative activity in IVM oocytes. SNAP treatment during IVM showed a stimulating effect on in vitro development of SCNT embryos (Experiment 7). These results demonstrates that SNAP improves developmental competence of PA and SCNT embryos probably by maintaining the redox homeostasis through increasing GSH content and mitochondrial quality and decreasing ROS in IVM oocyteshttp://www.e-jarb.org/journal/view.html?uid=68&vmd=Fullnitric oxidesnapoocyte maturationembryonic developmentpig
spellingShingle Fazle Elahi
Hyeji Shin
Joohyeong Lee
Seung Tae Lee
Geun-Shik Lee
Eunsong Lee
Exogenous Nitric Oxide Donation During In Vitro Maturation Improves Embryonic Development after Parthenogenesis and Somatic Cell Nuclear Transfer in Pigs
Journal of Animal Reproduction and Biotechnology
nitric oxide
snap
oocyte maturation
embryonic development
pig
title Exogenous Nitric Oxide Donation During In Vitro Maturation Improves Embryonic Development after Parthenogenesis and Somatic Cell Nuclear Transfer in Pigs
title_full Exogenous Nitric Oxide Donation During In Vitro Maturation Improves Embryonic Development after Parthenogenesis and Somatic Cell Nuclear Transfer in Pigs
title_fullStr Exogenous Nitric Oxide Donation During In Vitro Maturation Improves Embryonic Development after Parthenogenesis and Somatic Cell Nuclear Transfer in Pigs
title_full_unstemmed Exogenous Nitric Oxide Donation During In Vitro Maturation Improves Embryonic Development after Parthenogenesis and Somatic Cell Nuclear Transfer in Pigs
title_short Exogenous Nitric Oxide Donation During In Vitro Maturation Improves Embryonic Development after Parthenogenesis and Somatic Cell Nuclear Transfer in Pigs
title_sort exogenous nitric oxide donation during in vitro maturation improves embryonic development after parthenogenesis and somatic cell nuclear transfer in pigs
topic nitric oxide
snap
oocyte maturation
embryonic development
pig
url http://www.e-jarb.org/journal/view.html?uid=68&vmd=Full
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AT joohyeonglee exogenousnitricoxidedonationduringinvitromaturationimprovesembryonicdevelopmentafterparthenogenesisandsomaticcellnucleartransferinpigs
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