Exogenous Nitric Oxide Donation During In Vitro Maturation Improves Embryonic Development after Parthenogenesis and Somatic Cell Nuclear Transfer in Pigs
Nitric oxide (NO) has an important role in oocyte maturation and embryonic development in mammals. This study examined the effect of exogenous NO donor S-nitroso-N-acetylpenicillamine (SNAP) in a maturation medium on meiotic progression and embryonic development after parthenogenesis (PA) and somati...
Main Authors: | , , , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
The Korean Society of Animal Reproduction and Biotechnology
2018-12-01
|
Series: | Journal of Animal Reproduction and Biotechnology |
Subjects: | |
Online Access: | http://www.e-jarb.org/journal/view.html?uid=68&vmd=Full |
_version_ | 1818871063632674816 |
---|---|
author | Fazle Elahi Hyeji Shin Joohyeong Lee Seung Tae Lee Geun-Shik Lee Eunsong Lee |
author_facet | Fazle Elahi Hyeji Shin Joohyeong Lee Seung Tae Lee Geun-Shik Lee Eunsong Lee |
author_sort | Fazle Elahi |
collection | DOAJ |
description | Nitric oxide (NO) has an important role in oocyte maturation and embryonic development in mammals. This study examined the effect of exogenous NO donor S-nitroso-N-acetylpenicillamine (SNAP) in a maturation medium on meiotic progression and embryonic development after parthenogenesis (PA) and somatic cell nuclear transfer (SCNT) in pigs. When oocytes were exposed to 0.1 μM SNAP for first 22 h of in vitro maturation (IVM) in Experiment 1, SNAP significantly improved blastocyst development in both defined and standard follicular fluid-supplemented media compared to untreated control (48.4 vs. 31.7-42.5%). SNAP treatment significantly arrested meiotic progression of oocytes at the germinal vesicle stage at 11 h of IVM (61.2 vs. 38.7%). However, there was no effect on meiotic progression at 22 h of IVM (Experiment 2). In Experiment 3, when oocytes were treated with SNAP at 0.001, 0.1 and 10 μM during the first 22 h of IVM to determine a suitable concentration, 0.1 μM SNAP (54.2%) exhibited a higher blastocyst formation than 0 and 10 μM SNAP (36.6 and 36.6%, respectively). Time-dependent effect of SNAP treatment was evaluated in Experiment 4. It was observed that SNAP treatment for the first 22 h of IVM significantly increased blastocyst formation compared to no treatment (57.1% vs. 46.2%). Antioxidant effect of SNAP was compared with that of cysteine. SNAP treatment significantly improved embryonic development to the blastocyst stage (49.1-51.5% vs. 34.4-37.5%) irrespective of the presence or absence of cysteine (Experiment 5). Moreover, SNAP significantly increased glutathione (GSH) content and inversely decreased the reactive oxygen species (ROS) level and mitochondrial oxidative activity in IVM oocytes. SNAP treatment during IVM showed a stimulating effect on in vitro development of SCNT embryos (Experiment 7). These results demonstrates that SNAP improves developmental competence of PA and SCNT embryos probably by maintaining the redox homeostasis through increasing GSH content and mitochondrial quality and decreasing ROS in IVM oocytes |
first_indexed | 2024-12-19T12:16:58Z |
format | Article |
id | doaj.art-a39708c515014a3f81715e679c27e845 |
institution | Directory Open Access Journal |
issn | 2671-4639 2671-4663 |
language | English |
last_indexed | 2024-12-19T12:16:58Z |
publishDate | 2018-12-01 |
publisher | The Korean Society of Animal Reproduction and Biotechnology |
record_format | Article |
series | Journal of Animal Reproduction and Biotechnology |
spelling | doaj.art-a39708c515014a3f81715e679c27e8452022-12-21T20:21:55ZengThe Korean Society of Animal Reproduction and BiotechnologyJournal of Animal Reproduction and Biotechnology2671-46392671-46632018-12-0133421122010.12750/JET.2018.33.4.211Exogenous Nitric Oxide Donation During In Vitro Maturation Improves Embryonic Development after Parthenogenesis and Somatic Cell Nuclear Transfer in PigsFazle Elahi0Hyeji Shin1Joohyeong Lee2Seung Tae Lee3Geun-Shik Lee4Eunsong Lee5College of Veterinary Medicine, Kangwon National University, Gangwon 24341, KoreaCollege of Veterinary Medicine, Kangwon National University, Gangwon 24341, KoreaInstitute of Veterinary Science, Kangwon National University, Gangwon 24341, KoreaDivision of Applied Animal Science, College of Animal Life Science, Kangwon National University, Gangwon 24341, KoreaCollege of Veterinary Medicine, Kangwon National University, Gangwon 24341, KoreCollege of Veterinary Medicine, Kangwon National University, Gangwon 24341, KoreaNitric oxide (NO) has an important role in oocyte maturation and embryonic development in mammals. This study examined the effect of exogenous NO donor S-nitroso-N-acetylpenicillamine (SNAP) in a maturation medium on meiotic progression and embryonic development after parthenogenesis (PA) and somatic cell nuclear transfer (SCNT) in pigs. When oocytes were exposed to 0.1 μM SNAP for first 22 h of in vitro maturation (IVM) in Experiment 1, SNAP significantly improved blastocyst development in both defined and standard follicular fluid-supplemented media compared to untreated control (48.4 vs. 31.7-42.5%). SNAP treatment significantly arrested meiotic progression of oocytes at the germinal vesicle stage at 11 h of IVM (61.2 vs. 38.7%). However, there was no effect on meiotic progression at 22 h of IVM (Experiment 2). In Experiment 3, when oocytes were treated with SNAP at 0.001, 0.1 and 10 μM during the first 22 h of IVM to determine a suitable concentration, 0.1 μM SNAP (54.2%) exhibited a higher blastocyst formation than 0 and 10 μM SNAP (36.6 and 36.6%, respectively). Time-dependent effect of SNAP treatment was evaluated in Experiment 4. It was observed that SNAP treatment for the first 22 h of IVM significantly increased blastocyst formation compared to no treatment (57.1% vs. 46.2%). Antioxidant effect of SNAP was compared with that of cysteine. SNAP treatment significantly improved embryonic development to the blastocyst stage (49.1-51.5% vs. 34.4-37.5%) irrespective of the presence or absence of cysteine (Experiment 5). Moreover, SNAP significantly increased glutathione (GSH) content and inversely decreased the reactive oxygen species (ROS) level and mitochondrial oxidative activity in IVM oocytes. SNAP treatment during IVM showed a stimulating effect on in vitro development of SCNT embryos (Experiment 7). These results demonstrates that SNAP improves developmental competence of PA and SCNT embryos probably by maintaining the redox homeostasis through increasing GSH content and mitochondrial quality and decreasing ROS in IVM oocyteshttp://www.e-jarb.org/journal/view.html?uid=68&vmd=Fullnitric oxidesnapoocyte maturationembryonic developmentpig |
spellingShingle | Fazle Elahi Hyeji Shin Joohyeong Lee Seung Tae Lee Geun-Shik Lee Eunsong Lee Exogenous Nitric Oxide Donation During In Vitro Maturation Improves Embryonic Development after Parthenogenesis and Somatic Cell Nuclear Transfer in Pigs Journal of Animal Reproduction and Biotechnology nitric oxide snap oocyte maturation embryonic development pig |
title | Exogenous Nitric Oxide Donation During In Vitro Maturation Improves Embryonic Development after Parthenogenesis and Somatic Cell Nuclear Transfer in Pigs |
title_full | Exogenous Nitric Oxide Donation During In Vitro Maturation Improves Embryonic Development after Parthenogenesis and Somatic Cell Nuclear Transfer in Pigs |
title_fullStr | Exogenous Nitric Oxide Donation During In Vitro Maturation Improves Embryonic Development after Parthenogenesis and Somatic Cell Nuclear Transfer in Pigs |
title_full_unstemmed | Exogenous Nitric Oxide Donation During In Vitro Maturation Improves Embryonic Development after Parthenogenesis and Somatic Cell Nuclear Transfer in Pigs |
title_short | Exogenous Nitric Oxide Donation During In Vitro Maturation Improves Embryonic Development after Parthenogenesis and Somatic Cell Nuclear Transfer in Pigs |
title_sort | exogenous nitric oxide donation during in vitro maturation improves embryonic development after parthenogenesis and somatic cell nuclear transfer in pigs |
topic | nitric oxide snap oocyte maturation embryonic development pig |
url | http://www.e-jarb.org/journal/view.html?uid=68&vmd=Full |
work_keys_str_mv | AT fazleelahi exogenousnitricoxidedonationduringinvitromaturationimprovesembryonicdevelopmentafterparthenogenesisandsomaticcellnucleartransferinpigs AT hyejishin exogenousnitricoxidedonationduringinvitromaturationimprovesembryonicdevelopmentafterparthenogenesisandsomaticcellnucleartransferinpigs AT joohyeonglee exogenousnitricoxidedonationduringinvitromaturationimprovesembryonicdevelopmentafterparthenogenesisandsomaticcellnucleartransferinpigs AT seungtaelee exogenousnitricoxidedonationduringinvitromaturationimprovesembryonicdevelopmentafterparthenogenesisandsomaticcellnucleartransferinpigs AT geunshiklee exogenousnitricoxidedonationduringinvitromaturationimprovesembryonicdevelopmentafterparthenogenesisandsomaticcellnucleartransferinpigs AT eunsonglee exogenousnitricoxidedonationduringinvitromaturationimprovesembryonicdevelopmentafterparthenogenesisandsomaticcellnucleartransferinpigs |