Flavones Isolated from Scutellariae radix Suppress Propionibacterium Acnes-Induced Cytokine Production In Vitro and In Vivo
Scutellariae radix, the root of Scutellaria baicalensis, has long been applied in traditional formulations and modern herbal medications. Propionibacterium acnes (P. acnes) in follicles can trigger inflammation and lead to the symptom of inflammatory acnes vulgaris. This study was aimed at evaluatin...
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MDPI AG
2015-12-01
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| Series: | Molecules |
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| author | Po-Jung Tsai Wen-Cheng Huang Ming-Chi Hsieh Ping-Jyun Sung Yueh-Hsiung Kuo Wen-Huey Wu |
| author_facet | Po-Jung Tsai Wen-Cheng Huang Ming-Chi Hsieh Ping-Jyun Sung Yueh-Hsiung Kuo Wen-Huey Wu |
| author_sort | Po-Jung Tsai |
| collection | DOAJ |
| description | Scutellariae radix, the root of Scutellaria baicalensis, has long been applied in traditional formulations and modern herbal medications. Propionibacterium acnes (P. acnes) in follicles can trigger inflammation and lead to the symptom of inflammatory acnes vulgaris. This study was aimed at evaluating the effect of Scutellariae radix extract and purified components isolated from it on inflammation induced by P. acnes in vitro and in vivo. The results showed the ethyl acetate (EA) soluble fraction from the partition of crude ethanolic extract from Scutellariae radix inhibited P. acnes-induced interleukin IL-8 and IL-1β production in human monocytic THP-1 cells. Seven flavones were isolated from the EA fraction by repeated chromatographies, and identified as 5,7-dihydroxy-6-methoxyflavone (FL1, oroxylin), 5,7-dihydroxy-8-methoxyflavone (FL2, wogonin), 5-hydroxy-7,8-dimethoxyflavone (FL3, 7-O-methylwogonin), 5,6′-dihydroxy-6,7,8,2′-tetramethoxy flavone (FL4, skullcapflavone II), 5,7,4′-trihydroxy-8-methoxyflavone (FL5), 5,2′,6′-trihydroxy-7,8-dimethoxyflavone (FL6, viscidulin II), and 5,7,2′,5′-tetrahydroxy-8,6′-dimethoxyflavone (FL7, ganhuangenin). They all significantly suppressed P. acnes-induced IL-8 and IL-1β production in THP-1 cells, and FL2 exerted the strongest effect with half maximal inhibition (IC50) values of 8.7 and 4.9 μM, respectively. Concomitant intradermal injection of each of the seven flavones (20 μg) with P. acnes effectively attenuated P. acnes-induced ear swelling, and decreased the production of IL-6 and tumor necrosis factor-α in ear homogenates. Our results suggested that all the seven flavones can be potential therapeutic agents against P. acnes-induced skin inflammation. |
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| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-12-22T22:28:19Z |
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| spelling | doaj.art-a3995025026041a8ad20d635718547882022-12-21T18:10:29ZengMDPI AGMolecules1420-30492015-12-012111510.3390/molecules21010015molecules21010015Flavones Isolated from Scutellariae radix Suppress Propionibacterium Acnes-Induced Cytokine Production In Vitro and In VivoPo-Jung Tsai0Wen-Cheng Huang1Ming-Chi Hsieh2Ping-Jyun Sung3Yueh-Hsiung Kuo4Wen-Huey Wu5Department of Human Development and Family Studies, National Taiwan Normal University, Taipei 106, TaiwanDepartment of Human Development and Family Studies, National Taiwan Normal University, Taipei 106, TaiwanDepartment of Human Development and Family Studies, National Taiwan Normal University, Taipei 106, TaiwanNational Museum of Marine Biology and Aquarium, Pingtung 944, TaiwanDepartment of Chinese Pharmaceutical Sciences and Chinese Medicine Resources, China Medical University, Taichung 404, TaiwanDepartment of Human Development and Family Studies, National Taiwan Normal University, Taipei 106, TaiwanScutellariae radix, the root of Scutellaria baicalensis, has long been applied in traditional formulations and modern herbal medications. Propionibacterium acnes (P. acnes) in follicles can trigger inflammation and lead to the symptom of inflammatory acnes vulgaris. This study was aimed at evaluating the effect of Scutellariae radix extract and purified components isolated from it on inflammation induced by P. acnes in vitro and in vivo. The results showed the ethyl acetate (EA) soluble fraction from the partition of crude ethanolic extract from Scutellariae radix inhibited P. acnes-induced interleukin IL-8 and IL-1β production in human monocytic THP-1 cells. Seven flavones were isolated from the EA fraction by repeated chromatographies, and identified as 5,7-dihydroxy-6-methoxyflavone (FL1, oroxylin), 5,7-dihydroxy-8-methoxyflavone (FL2, wogonin), 5-hydroxy-7,8-dimethoxyflavone (FL3, 7-O-methylwogonin), 5,6′-dihydroxy-6,7,8,2′-tetramethoxy flavone (FL4, skullcapflavone II), 5,7,4′-trihydroxy-8-methoxyflavone (FL5), 5,2′,6′-trihydroxy-7,8-dimethoxyflavone (FL6, viscidulin II), and 5,7,2′,5′-tetrahydroxy-8,6′-dimethoxyflavone (FL7, ganhuangenin). They all significantly suppressed P. acnes-induced IL-8 and IL-1β production in THP-1 cells, and FL2 exerted the strongest effect with half maximal inhibition (IC50) values of 8.7 and 4.9 μM, respectively. Concomitant intradermal injection of each of the seven flavones (20 μg) with P. acnes effectively attenuated P. acnes-induced ear swelling, and decreased the production of IL-6 and tumor necrosis factor-α in ear homogenates. Our results suggested that all the seven flavones can be potential therapeutic agents against P. acnes-induced skin inflammation.http://www.mdpi.com/1420-3049/21/1/15Chinese herbScutellariae radixflavoneanti-inflammationPropionibacterium acnes |
| spellingShingle | Po-Jung Tsai Wen-Cheng Huang Ming-Chi Hsieh Ping-Jyun Sung Yueh-Hsiung Kuo Wen-Huey Wu Flavones Isolated from Scutellariae radix Suppress Propionibacterium Acnes-Induced Cytokine Production In Vitro and In Vivo Molecules Chinese herb Scutellariae radix flavone anti-inflammation Propionibacterium acnes |
| title | Flavones Isolated from Scutellariae radix Suppress Propionibacterium Acnes-Induced Cytokine Production In Vitro and In Vivo |
| title_full | Flavones Isolated from Scutellariae radix Suppress Propionibacterium Acnes-Induced Cytokine Production In Vitro and In Vivo |
| title_fullStr | Flavones Isolated from Scutellariae radix Suppress Propionibacterium Acnes-Induced Cytokine Production In Vitro and In Vivo |
| title_full_unstemmed | Flavones Isolated from Scutellariae radix Suppress Propionibacterium Acnes-Induced Cytokine Production In Vitro and In Vivo |
| title_short | Flavones Isolated from Scutellariae radix Suppress Propionibacterium Acnes-Induced Cytokine Production In Vitro and In Vivo |
| title_sort | flavones isolated from scutellariae radix suppress propionibacterium acnes induced cytokine production in vitro and in vivo |
| topic | Chinese herb Scutellariae radix flavone anti-inflammation Propionibacterium acnes |
| url | http://www.mdpi.com/1420-3049/21/1/15 |
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