Circular RNA circ_0006168 enhances Taxol resistance in esophageal squamous cell carcinoma by regulating miR-194-5p/JMJD1C axis
Abstract Background Chemoresistance is one of the major obstacles for cancer therapy in the clinic. Circular RNAs (circRNAs) are involved in the pathogenesis of esophageal squamous cell carcinoma (ESCC) and chemoresistance. This study aimed to explore the role and molecular mechanism of circ_0006168...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
BMC
2021-05-01
|
| Series: | Cancer Cell International |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s12935-021-01984-y |
| _version_ | 1818455041204289536 |
|---|---|
| author | Fanyong Qu Lina Wang Caiyan Wang Lingxia Yu Kaikai Zhao Hao Zhong |
| author_facet | Fanyong Qu Lina Wang Caiyan Wang Lingxia Yu Kaikai Zhao Hao Zhong |
| author_sort | Fanyong Qu |
| collection | DOAJ |
| description | Abstract Background Chemoresistance is one of the major obstacles for cancer therapy in the clinic. Circular RNAs (circRNAs) are involved in the pathogenesis of esophageal squamous cell carcinoma (ESCC) and chemoresistance. This study aimed to explore the role and molecular mechanism of circ_0006168 in Taxol resistance of ESCC. Methods The expression levels of circ_0006168, microRNA-194-5p (miR-194-5p) and jumonji domain containing 1C (JMJD1C) were measured by quantitative real-time polymerase chain reaction (qRT-PCR) or western blot. The half-inhibition concentration (IC50) value of Taxol was evaluated by Cell Counting Kit-8 (CCK-8) assay. Cell proliferation was evaluated by CCK-8 and colony formation assays. Cell migration and invasion were detected by transwell assay. Cell apoptosis was determined by flow cytometry. The interaction between miR-194-5p and circ_0006168 or JMJD1C was predicted by bioinformatics analysis (Circinteractome and TargetScan) and verified by dual-luciferase reporter and RNA Immunoprecipitation (RIP) and RNA pull-down assays. The mice xenograft model was established to investigate the roles of circ_0006168 in vivo. Results Circ_0006168 and JMJD1C were upregulated and miR-194-5p was downregulated in ESCC tissues, ESCC cells, and Taxol-resistant cells. Functionally, knockdown of circ_0006168 or JMJD1C increased Taxol sensitivity of ESCC in vitro via inhibiting cell proliferation, migration and invasion, and promoting apoptosis. Moreover, circ_0006168 could directly bind to miR-194-5p and JMJD1C was verified as a direct target of miR-194-5p. Mechanically, circ_0006168 was a sponge of miR-194-5p to regulate JMJD1C expression in ESCC cells. Furthermore, JMJD1C overexpression reversed the promotive effect of circ_0006168 knockdown on Taxol sensitivity. Besides, circ_0006168 silence suppressed tumor growth in vivo. Conclusion Circ_0006168 facilitated Taxol resistance in ESCC by regulating miR-194-5p/JMJD1C axis, providing a promising therapeutic target for ESCC chemotherapy. |
| first_indexed | 2024-12-14T22:04:28Z |
| format | Article |
| id | doaj.art-a39bec907919427fb94227561fa30afc |
| institution | Directory Open Access Journal |
| issn | 1475-2867 |
| language | English |
| last_indexed | 2024-12-14T22:04:28Z |
| publishDate | 2021-05-01 |
| publisher | BMC |
| record_format | Article |
| series | Cancer Cell International |
| spelling | doaj.art-a39bec907919427fb94227561fa30afc2022-12-21T22:45:54ZengBMCCancer Cell International1475-28672021-05-0121111210.1186/s12935-021-01984-yCircular RNA circ_0006168 enhances Taxol resistance in esophageal squamous cell carcinoma by regulating miR-194-5p/JMJD1C axisFanyong Qu0Lina Wang1Caiyan Wang2Lingxia Yu3Kaikai Zhao4Hao Zhong5Department of Radiation Oncology, Yantai Affiliated Hospital of Binzhou Medical UniversityDepartment of Oncology, Yantai Affiliated Hospital of Binzhou Medical UniversityDepartment of Gastroenterology, Yantai Affiliated Hospital of Binzhou Medical UniversityDepartment of Oncology, Yantai Affiliated Hospital of Binzhou Medical UniversityDepartment of Radiation Oncology, Yantai Affiliated Hospital of Binzhou Medical UniversityDepartment of Radiation Oncology, Yantai Affiliated Hospital of Binzhou Medical UniversityAbstract Background Chemoresistance is one of the major obstacles for cancer therapy in the clinic. Circular RNAs (circRNAs) are involved in the pathogenesis of esophageal squamous cell carcinoma (ESCC) and chemoresistance. This study aimed to explore the role and molecular mechanism of circ_0006168 in Taxol resistance of ESCC. Methods The expression levels of circ_0006168, microRNA-194-5p (miR-194-5p) and jumonji domain containing 1C (JMJD1C) were measured by quantitative real-time polymerase chain reaction (qRT-PCR) or western blot. The half-inhibition concentration (IC50) value of Taxol was evaluated by Cell Counting Kit-8 (CCK-8) assay. Cell proliferation was evaluated by CCK-8 and colony formation assays. Cell migration and invasion were detected by transwell assay. Cell apoptosis was determined by flow cytometry. The interaction between miR-194-5p and circ_0006168 or JMJD1C was predicted by bioinformatics analysis (Circinteractome and TargetScan) and verified by dual-luciferase reporter and RNA Immunoprecipitation (RIP) and RNA pull-down assays. The mice xenograft model was established to investigate the roles of circ_0006168 in vivo. Results Circ_0006168 and JMJD1C were upregulated and miR-194-5p was downregulated in ESCC tissues, ESCC cells, and Taxol-resistant cells. Functionally, knockdown of circ_0006168 or JMJD1C increased Taxol sensitivity of ESCC in vitro via inhibiting cell proliferation, migration and invasion, and promoting apoptosis. Moreover, circ_0006168 could directly bind to miR-194-5p and JMJD1C was verified as a direct target of miR-194-5p. Mechanically, circ_0006168 was a sponge of miR-194-5p to regulate JMJD1C expression in ESCC cells. Furthermore, JMJD1C overexpression reversed the promotive effect of circ_0006168 knockdown on Taxol sensitivity. Besides, circ_0006168 silence suppressed tumor growth in vivo. Conclusion Circ_0006168 facilitated Taxol resistance in ESCC by regulating miR-194-5p/JMJD1C axis, providing a promising therapeutic target for ESCC chemotherapy.https://doi.org/10.1186/s12935-021-01984-yEsophageal squamous cell carcinomaTaxol resistancecirc_0006168miR-194-5pJMJD1C |
| spellingShingle | Fanyong Qu Lina Wang Caiyan Wang Lingxia Yu Kaikai Zhao Hao Zhong Circular RNA circ_0006168 enhances Taxol resistance in esophageal squamous cell carcinoma by regulating miR-194-5p/JMJD1C axis Cancer Cell International Esophageal squamous cell carcinoma Taxol resistance circ_0006168 miR-194-5p JMJD1C |
| title | Circular RNA circ_0006168 enhances Taxol resistance in esophageal squamous cell carcinoma by regulating miR-194-5p/JMJD1C axis |
| title_full | Circular RNA circ_0006168 enhances Taxol resistance in esophageal squamous cell carcinoma by regulating miR-194-5p/JMJD1C axis |
| title_fullStr | Circular RNA circ_0006168 enhances Taxol resistance in esophageal squamous cell carcinoma by regulating miR-194-5p/JMJD1C axis |
| title_full_unstemmed | Circular RNA circ_0006168 enhances Taxol resistance in esophageal squamous cell carcinoma by regulating miR-194-5p/JMJD1C axis |
| title_short | Circular RNA circ_0006168 enhances Taxol resistance in esophageal squamous cell carcinoma by regulating miR-194-5p/JMJD1C axis |
| title_sort | circular rna circ 0006168 enhances taxol resistance in esophageal squamous cell carcinoma by regulating mir 194 5p jmjd1c axis |
| topic | Esophageal squamous cell carcinoma Taxol resistance circ_0006168 miR-194-5p JMJD1C |
| url | https://doi.org/10.1186/s12935-021-01984-y |
| work_keys_str_mv | AT fanyongqu circularrnacirc0006168enhancestaxolresistanceinesophagealsquamouscellcarcinomabyregulatingmir1945pjmjd1caxis AT linawang circularrnacirc0006168enhancestaxolresistanceinesophagealsquamouscellcarcinomabyregulatingmir1945pjmjd1caxis AT caiyanwang circularrnacirc0006168enhancestaxolresistanceinesophagealsquamouscellcarcinomabyregulatingmir1945pjmjd1caxis AT lingxiayu circularrnacirc0006168enhancestaxolresistanceinesophagealsquamouscellcarcinomabyregulatingmir1945pjmjd1caxis AT kaikaizhao circularrnacirc0006168enhancestaxolresistanceinesophagealsquamouscellcarcinomabyregulatingmir1945pjmjd1caxis AT haozhong circularrnacirc0006168enhancestaxolresistanceinesophagealsquamouscellcarcinomabyregulatingmir1945pjmjd1caxis |