Evaluation of CD4+CD25+ T lymphocyte response time kinetics in patients with chronic Chagas disease after in vitro stimulation with recombinant Trypanosoma cruzi antigens
Introduction CD4+CD25+ T lymphocytes have been implicated in the regulation of host inflammatory response against Trypanosoma cruzi, and may be involved in the clinical course of the disease. Methods Peripheral blood mononuclear cells from patients with chronic Chagas disease were cultured in the...
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| Format: | Article |
| Language: | English |
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Sociedade Brasileira de Medicina Tropical (SBMT)
2013-10-01
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| Series: | Revista da Sociedade Brasileira de Medicina Tropical |
| Subjects: | |
| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0037-86822013005000015 |
| _version_ | 1828338381177749504 |
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| author | Suellen Carvalho de Moura Braz Virginia Maria Barros de Lorena Adriene Siqueira Melo Maria da Glória Aureliano Melo Cavalcanti Yara de Miranda Gomes |
| author_facet | Suellen Carvalho de Moura Braz Virginia Maria Barros de Lorena Adriene Siqueira Melo Maria da Glória Aureliano Melo Cavalcanti Yara de Miranda Gomes |
| author_sort | Suellen Carvalho de Moura Braz |
| collection | DOAJ |
| description | Introduction CD4+CD25+ T lymphocytes have been implicated in the regulation of host inflammatory response against Trypanosoma cruzi, and may be involved in the clinical course of the disease. Methods Peripheral blood mononuclear cells from patients with chronic Chagas disease were cultured in the presence of T. cruzi recombinant antigens and assayed for lymphocytes at distinct time points. Results It was possible to differentiate clinical forms of chronic Chagas disease at days 3 and 5 according to presence of CD4+CD25+ T cells in cell cultures. Conclusions Longer periods of cell culture proved to be potentially valuable for prospective evaluations of CD4+CD25+ T lymphocytes in patients with chronic Chagas disease. |
| first_indexed | 2024-04-13T22:27:03Z |
| format | Article |
| id | doaj.art-a39fa35aec8d4f4aaa914a73fc2eb985 |
| institution | Directory Open Access Journal |
| issn | 0037-8682 1678-9849 |
| language | English |
| last_indexed | 2024-04-13T22:27:03Z |
| publishDate | 2013-10-01 |
| publisher | Sociedade Brasileira de Medicina Tropical (SBMT) |
| record_format | Article |
| series | Revista da Sociedade Brasileira de Medicina Tropical |
| spelling | doaj.art-a39fa35aec8d4f4aaa914a73fc2eb9852022-12-22T02:27:02ZengSociedade Brasileira de Medicina Tropical (SBMT)Revista da Sociedade Brasileira de Medicina Tropical0037-86821678-98492013-10-01ahead0000Evaluation of CD4+CD25+ T lymphocyte response time kinetics in patients with chronic Chagas disease after in vitro stimulation with recombinant Trypanosoma cruzi antigensSuellen Carvalho de Moura BrazVirginia Maria Barros de LorenaAdriene Siqueira MeloMaria da Glória Aureliano Melo CavalcantiYara de Miranda GomesIntroduction CD4+CD25+ T lymphocytes have been implicated in the regulation of host inflammatory response against Trypanosoma cruzi, and may be involved in the clinical course of the disease. Methods Peripheral blood mononuclear cells from patients with chronic Chagas disease were cultured in the presence of T. cruzi recombinant antigens and assayed for lymphocytes at distinct time points. Results It was possible to differentiate clinical forms of chronic Chagas disease at days 3 and 5 according to presence of CD4+CD25+ T cells in cell cultures. Conclusions Longer periods of cell culture proved to be potentially valuable for prospective evaluations of CD4+CD25+ T lymphocytes in patients with chronic Chagas disease.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0037-86822013005000015Chronic Chagas diseaseRecombinant antigensCD4+CD25+ T lymphocytes |
| spellingShingle | Suellen Carvalho de Moura Braz Virginia Maria Barros de Lorena Adriene Siqueira Melo Maria da Glória Aureliano Melo Cavalcanti Yara de Miranda Gomes Evaluation of CD4+CD25+ T lymphocyte response time kinetics in patients with chronic Chagas disease after in vitro stimulation with recombinant Trypanosoma cruzi antigens Revista da Sociedade Brasileira de Medicina Tropical Chronic Chagas disease Recombinant antigens CD4+CD25+ T lymphocytes |
| title | Evaluation of CD4+CD25+ T lymphocyte response time kinetics in patients with chronic Chagas disease after in vitro stimulation with recombinant Trypanosoma cruzi antigens |
| title_full | Evaluation of CD4+CD25+ T lymphocyte response time kinetics in patients with chronic Chagas disease after in vitro stimulation with recombinant Trypanosoma cruzi antigens |
| title_fullStr | Evaluation of CD4+CD25+ T lymphocyte response time kinetics in patients with chronic Chagas disease after in vitro stimulation with recombinant Trypanosoma cruzi antigens |
| title_full_unstemmed | Evaluation of CD4+CD25+ T lymphocyte response time kinetics in patients with chronic Chagas disease after in vitro stimulation with recombinant Trypanosoma cruzi antigens |
| title_short | Evaluation of CD4+CD25+ T lymphocyte response time kinetics in patients with chronic Chagas disease after in vitro stimulation with recombinant Trypanosoma cruzi antigens |
| title_sort | evaluation of cd4 cd25 t lymphocyte response time kinetics in patients with chronic chagas disease after in vitro stimulation with recombinant trypanosoma cruzi antigens |
| topic | Chronic Chagas disease Recombinant antigens CD4+CD25+ T lymphocytes |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0037-86822013005000015 |
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