Antimicrobial Activity of Metabolites Secreted by the Endophytic Bacterium <i>Frateuria defendens</i>
<i>Candidatus</i> Phytoplasma, the causative agent of yellows disease, inflicts substantial damage on several hundred plant species including perennials and annual plants. The endophytic bacterium <i>Frateuria defendens</i> reduces the symptoms of yellows disease in a number...
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MDPI AG
2020-01-01
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| Ráidu: | Plants |
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| Liŋkkat: | https://www.mdpi.com/2223-7747/9/1/72 |
| _version_ | 1831579463313260544 |
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| author | Alaa Naama-Amar Shani Gitman Nofar Shoshana Ofir Bahar Vered Naor Einat Zchori-Fein Lilach Iasur-Kruh |
| author_facet | Alaa Naama-Amar Shani Gitman Nofar Shoshana Ofir Bahar Vered Naor Einat Zchori-Fein Lilach Iasur-Kruh |
| author_sort | Alaa Naama-Amar |
| collection | DOAJ |
| description | <i>Candidatus</i> Phytoplasma, the causative agent of yellows disease, inflicts substantial damage on several hundred plant species including perennials and annual plants. The endophytic bacterium <i>Frateuria defendens</i> reduces the symptoms of yellows disease in a number of agricultural crops. One possible mode of action is that the bacterium secretes antimicrobial metabolites. To test this hypothesis, the substances secreted by the endophyte during 10 days of growth in an artificial medium were identified by GC-MS (gas chromatography−mass spectrometry). Synthetic analogues to these substances were then used on periwinkle, a nurse culture plant infected by phytoplasma. Phytoplasma quantities were evaluated by quantitative PCR, and disease symptoms were monitored and recorded. It was found that specific compounds identified by the biochemical analysis caused a significant reduction in both the titer of phytoplasma and the disease symptoms in periwinkle when compared to untreated infected plants. Further research is required to examine the potential of these compounds as an effective treatment against yellows disease. |
| first_indexed | 2024-12-17T16:29:30Z |
| format | Article |
| id | doaj.art-a3a2f5ee5a6e4380bab48c64d5c75ff1 |
| institution | Directory Open Access Journal |
| issn | 2223-7747 |
| language | English |
| last_indexed | 2024-12-17T16:29:30Z |
| publishDate | 2020-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Plants |
| spelling | doaj.art-a3a2f5ee5a6e4380bab48c64d5c75ff12022-12-21T21:41:18ZengMDPI AGPlants2223-77472020-01-01917210.3390/plants9010072plants9010072Antimicrobial Activity of Metabolites Secreted by the Endophytic Bacterium <i>Frateuria defendens</i>Alaa Naama-Amar0Shani Gitman1Nofar Shoshana2Ofir Bahar3Vered Naor4Einat Zchori-Fein5Lilach Iasur-Kruh6Department of Biotechnology Engineering, ORT Braude College of Engineering, Karmiel 2161002, IsraelDepartment of Entomology, Agricultural Research Organization, Newe Ya’ar Research Center, Bet Shearim 3657800, IsraelDepartment of Plant Pathology and Weed Research, Agricultural Research Organization, Volcani Center, Rishon LeZion 7534509, IsraelDepartment of Plant Pathology and Weed Research, Agricultural Research Organization, Volcani Center, Rishon LeZion 7534509, IsraelShamir Research Institute, Qatsrin 1290000, IsraelDepartment of Entomology, Agricultural Research Organization, Newe Ya’ar Research Center, Bet Shearim 3657800, IsraelDepartment of Biotechnology Engineering, ORT Braude College of Engineering, Karmiel 2161002, Israel<i>Candidatus</i> Phytoplasma, the causative agent of yellows disease, inflicts substantial damage on several hundred plant species including perennials and annual plants. The endophytic bacterium <i>Frateuria defendens</i> reduces the symptoms of yellows disease in a number of agricultural crops. One possible mode of action is that the bacterium secretes antimicrobial metabolites. To test this hypothesis, the substances secreted by the endophyte during 10 days of growth in an artificial medium were identified by GC-MS (gas chromatography−mass spectrometry). Synthetic analogues to these substances were then used on periwinkle, a nurse culture plant infected by phytoplasma. Phytoplasma quantities were evaluated by quantitative PCR, and disease symptoms were monitored and recorded. It was found that specific compounds identified by the biochemical analysis caused a significant reduction in both the titer of phytoplasma and the disease symptoms in periwinkle when compared to untreated infected plants. Further research is required to examine the potential of these compounds as an effective treatment against yellows disease.https://www.mdpi.com/2223-7747/9/1/72<i>frateuria defendens</i>yellows diseaseendophytic bacteriaphytoplasma4-quinolinecarboxaldehyde5-hydroxymethyl-2-furaldehydemetabolites |
| spellingShingle | Alaa Naama-Amar Shani Gitman Nofar Shoshana Ofir Bahar Vered Naor Einat Zchori-Fein Lilach Iasur-Kruh Antimicrobial Activity of Metabolites Secreted by the Endophytic Bacterium <i>Frateuria defendens</i> Plants <i>frateuria defendens</i> yellows disease endophytic bacteria phytoplasma 4-quinolinecarboxaldehyde 5-hydroxymethyl-2-furaldehyde metabolites |
| title | Antimicrobial Activity of Metabolites Secreted by the Endophytic Bacterium <i>Frateuria defendens</i> |
| title_full | Antimicrobial Activity of Metabolites Secreted by the Endophytic Bacterium <i>Frateuria defendens</i> |
| title_fullStr | Antimicrobial Activity of Metabolites Secreted by the Endophytic Bacterium <i>Frateuria defendens</i> |
| title_full_unstemmed | Antimicrobial Activity of Metabolites Secreted by the Endophytic Bacterium <i>Frateuria defendens</i> |
| title_short | Antimicrobial Activity of Metabolites Secreted by the Endophytic Bacterium <i>Frateuria defendens</i> |
| title_sort | antimicrobial activity of metabolites secreted by the endophytic bacterium i frateuria defendens i |
| topic | <i>frateuria defendens</i> yellows disease endophytic bacteria phytoplasma 4-quinolinecarboxaldehyde 5-hydroxymethyl-2-furaldehyde metabolites |
| url | https://www.mdpi.com/2223-7747/9/1/72 |
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