A Simplified Model of Adenine-Induced Chronic Kidney Disease Using SKH1 Mice
Commonly used adenine-induced chronic kidney disease (CKD) murine models often employ C57BL/6 mice; however, this strain has inherent limitations due to its natural resistance to developing key pathological features of CKD, such as tubulointerstitial fibrosis and inflammation. There have been attemp...
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| Format: | Article |
| Jezik: | English |
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MDPI AG
2024-12-01
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| Serija: | Cells |
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| Online dostop: | https://www.mdpi.com/2073-4409/13/24/2117 |
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| author | Benjamin W. French Joshua D. Breidenbach Shereen G. Yassine Bella Z. Khatib-Shahidi Sara Kazmi Caitlin M. Murphy Humza S. Bashir Evan M. Benson Bivek Timalsina Upasana Shrestha Dhilhani Faleel Satkeerth Boyapalli Prabhatchandra Dube Apurva Lad Irum Syed Deepak Malhotra Amira Gohara David J. Kennedy Steven T. Haller |
| author_facet | Benjamin W. French Joshua D. Breidenbach Shereen G. Yassine Bella Z. Khatib-Shahidi Sara Kazmi Caitlin M. Murphy Humza S. Bashir Evan M. Benson Bivek Timalsina Upasana Shrestha Dhilhani Faleel Satkeerth Boyapalli Prabhatchandra Dube Apurva Lad Irum Syed Deepak Malhotra Amira Gohara David J. Kennedy Steven T. Haller |
| author_sort | Benjamin W. French |
| collection | DOAJ |
| description | Commonly used adenine-induced chronic kidney disease (CKD) murine models often employ C57BL/6 mice; however, this strain has inherent limitations due to its natural resistance to developing key pathological features of CKD, such as tubulointerstitial fibrosis and inflammation. There have been attempts to overcome these barriers by using multiple concentrations of adenine-supplemented diets or by performing prolonged experiments up to 20 weeks. Here, we demonstrate that SKH1 Elite mice develop clinically relevant CKD phenotypes (e.g., polyuria, proteinuria, inflammation, and renal fibrosis) over the course of only 6 weeks of low-dose (0.15%) adenine supplementation. As a docile, immunocompetent, and hairless strain, SKH1 Elite mice offer several logistical advantages over C57BL/6 mice, including ease of handling and the ability to study dermal conditions, which are often secondary to CKD. |
| first_indexed | 2025-02-17T12:36:51Z |
| format | Article |
| id | doaj.art-a3a3c28f09f9446c8a4e6279a60b15b3 |
| institution | Directory Open Access Journal |
| issn | 2073-4409 |
| language | English |
| last_indexed | 2025-02-17T12:36:51Z |
| publishDate | 2024-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Cells |
| spelling | doaj.art-a3a3c28f09f9446c8a4e6279a60b15b32024-12-27T14:16:39ZengMDPI AGCells2073-44092024-12-011324211710.3390/cells13242117A Simplified Model of Adenine-Induced Chronic Kidney Disease Using SKH1 MiceBenjamin W. French0Joshua D. Breidenbach1Shereen G. Yassine2Bella Z. Khatib-Shahidi3Sara Kazmi4Caitlin M. Murphy5Humza S. Bashir6Evan M. Benson7Bivek Timalsina8Upasana Shrestha9Dhilhani Faleel10Satkeerth Boyapalli11Prabhatchandra Dube12Apurva Lad13Irum Syed14Deepak Malhotra15Amira Gohara16David J. Kennedy17Steven T. Haller18Department of Medicine, College of Medicine and Life Sciences, University of Toledo, Toledo, OH 43614, USADepartment of Medicine, College of Medicine and Life Sciences, University of Toledo, Toledo, OH 43614, USADepartment of Medicine, College of Medicine and Life Sciences, University of Toledo, Toledo, OH 43614, USADepartment of Medicine, College of Medicine and Life Sciences, University of Toledo, Toledo, OH 43614, USADepartment of Medicine, College of Medicine and Life Sciences, University of Toledo, Toledo, OH 43614, USADepartment of Medicine, College of Medicine and Life Sciences, University of Toledo, Toledo, OH 43614, USADepartment of Medicine, College of Medicine and Life Sciences, University of Toledo, Toledo, OH 43614, USADepartment of Medicine, College of Medicine and Life Sciences, University of Toledo, Toledo, OH 43614, USADepartment of Medicine, College of Medicine and Life Sciences, University of Toledo, Toledo, OH 43614, USADepartment of Medicine, College of Medicine and Life Sciences, University of Toledo, Toledo, OH 43614, USADepartment of Medicine, College of Medicine and Life Sciences, University of Toledo, Toledo, OH 43614, USADepartment of Medicine, College of Medicine and Life Sciences, University of Toledo, Toledo, OH 43614, USADepartment of Medicine, College of Medicine and Life Sciences, University of Toledo, Toledo, OH 43614, USADepartment of Medicine, College of Medicine and Life Sciences, University of Toledo, Toledo, OH 43614, USADepartment of Medical Microbiology and Immunology, College of Medicine and Life Sciences, University of Toledo, Toledo, OH 43614, USADepartment of Medicine, College of Medicine and Life Sciences, University of Toledo, Toledo, OH 43614, USADepartment of Pathology, College of Medicine and Life Sciences, University of Toledo, Toledo, OH 43614, USADepartment of Medicine, College of Medicine and Life Sciences, University of Toledo, Toledo, OH 43614, USADepartment of Medicine, College of Medicine and Life Sciences, University of Toledo, Toledo, OH 43614, USACommonly used adenine-induced chronic kidney disease (CKD) murine models often employ C57BL/6 mice; however, this strain has inherent limitations due to its natural resistance to developing key pathological features of CKD, such as tubulointerstitial fibrosis and inflammation. There have been attempts to overcome these barriers by using multiple concentrations of adenine-supplemented diets or by performing prolonged experiments up to 20 weeks. Here, we demonstrate that SKH1 Elite mice develop clinically relevant CKD phenotypes (e.g., polyuria, proteinuria, inflammation, and renal fibrosis) over the course of only 6 weeks of low-dose (0.15%) adenine supplementation. As a docile, immunocompetent, and hairless strain, SKH1 Elite mice offer several logistical advantages over C57BL/6 mice, including ease of handling and the ability to study dermal conditions, which are often secondary to CKD.https://www.mdpi.com/2073-4409/13/24/2117chronic kidney disease (CKD)adenine diet modelSKH1 elite micerenal pathologydermatological comorbidities |
| spellingShingle | Benjamin W. French Joshua D. Breidenbach Shereen G. Yassine Bella Z. Khatib-Shahidi Sara Kazmi Caitlin M. Murphy Humza S. Bashir Evan M. Benson Bivek Timalsina Upasana Shrestha Dhilhani Faleel Satkeerth Boyapalli Prabhatchandra Dube Apurva Lad Irum Syed Deepak Malhotra Amira Gohara David J. Kennedy Steven T. Haller A Simplified Model of Adenine-Induced Chronic Kidney Disease Using SKH1 Mice Cells chronic kidney disease (CKD) adenine diet model SKH1 elite mice renal pathology dermatological comorbidities |
| title | A Simplified Model of Adenine-Induced Chronic Kidney Disease Using SKH1 Mice |
| title_full | A Simplified Model of Adenine-Induced Chronic Kidney Disease Using SKH1 Mice |
| title_fullStr | A Simplified Model of Adenine-Induced Chronic Kidney Disease Using SKH1 Mice |
| title_full_unstemmed | A Simplified Model of Adenine-Induced Chronic Kidney Disease Using SKH1 Mice |
| title_short | A Simplified Model of Adenine-Induced Chronic Kidney Disease Using SKH1 Mice |
| title_sort | simplified model of adenine induced chronic kidney disease using skh1 mice |
| topic | chronic kidney disease (CKD) adenine diet model SKH1 elite mice renal pathology dermatological comorbidities |
| url | https://www.mdpi.com/2073-4409/13/24/2117 |
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