The Seed Development Factors <i>TT2</i> and <i>MYB5</i> Regulate Heat Stress Response in <i>Arabidopsis</i>
<i>HEAT SHOCK FACTOR A2</i> (<i>HSFA2</i>) is a regulator of multiple environmental stress responses required for stress acclimation. We analyzed <i>HSFA2</i> co-regulated genes and identified 43 genes strongly co-regulated with <i>HSFA2</i> during mul...
| Main Authors: | , , , , , , , , , , , , , |
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MDPI AG
2021-05-01
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| Series: | Genes |
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| Online Access: | https://www.mdpi.com/2073-4425/12/5/746 |
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| author | Pierre Jacob Gwilherm Brisou Marion Dalmais Johanne Thévenin Froukje van der Wal David Latrasse Ravi Suresh Devani Moussa Benhamed Bertrand Dubreucq Adnane Boualem Loic Lepiniec Richard G. H. Immink Heribert Hirt Abdelhafid Bendahmane |
| author_facet | Pierre Jacob Gwilherm Brisou Marion Dalmais Johanne Thévenin Froukje van der Wal David Latrasse Ravi Suresh Devani Moussa Benhamed Bertrand Dubreucq Adnane Boualem Loic Lepiniec Richard G. H. Immink Heribert Hirt Abdelhafid Bendahmane |
| author_sort | Pierre Jacob |
| collection | DOAJ |
| description | <i>HEAT SHOCK FACTOR A2</i> (<i>HSFA2</i>) is a regulator of multiple environmental stress responses required for stress acclimation. We analyzed <i>HSFA2</i> co-regulated genes and identified 43 genes strongly co-regulated with <i>HSFA2</i> during multiple stresses. Motif enrichment analysis revealed an over-representation of the site II element (SIIE) in the promoters of these genes. In a yeast 1-hybrid screen with the SIIE, we identified the closely related R2R3-MYB transcription factors TT2 and MYB5. We found overexpression of <i>MYB5</i> or <i>TT2</i> rendered plants heat stress tolerant. In contrast, <i>tt2</i>, <i>myb5</i>, and <i>tt2/myb5</i> loss of function mutants showed heat stress hypersensitivity. Transient expression assays confirmed that MYB5 and TT2 can regulate the <i>HSFA2</i> promoter together with the other members of the MBW complex, TT8 and TRANSPARENT TESTA GLABRA 1 (TTG1) and that the SIIE was involved in this regulation. Transcriptomic analysis revealed that <i>TT2/MYB5</i> target promoters were enriched in SIIE. Overall, we report a new function of TT2 and MYB5 in stress resistance and a role in SIIE-mediated <i>HSFA2</i> regulation. |
| first_indexed | 2024-03-10T11:23:10Z |
| format | Article |
| id | doaj.art-a3a44203673e4cc9bbb1b28f0da0ca15 |
| institution | Directory Open Access Journal |
| issn | 2073-4425 |
| language | English |
| last_indexed | 2024-03-10T11:23:10Z |
| publishDate | 2021-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Genes |
| spelling | doaj.art-a3a44203673e4cc9bbb1b28f0da0ca152023-11-21T19:52:29ZengMDPI AGGenes2073-44252021-05-0112574610.3390/genes12050746The Seed Development Factors <i>TT2</i> and <i>MYB5</i> Regulate Heat Stress Response in <i>Arabidopsis</i>Pierre Jacob0Gwilherm Brisou1Marion Dalmais2Johanne Thévenin3Froukje van der Wal4David Latrasse5Ravi Suresh Devani6Moussa Benhamed7Bertrand Dubreucq8Adnane Boualem9Loic Lepiniec10Richard G. H. Immink11Heribert Hirt12Abdelhafid Bendahmane13Institute of Plant Sciences Paris-Saclay, Université Paris-Saclay, Univ. Evry, INRAE, CNRS, 91405 Orsay, FranceInstitute of Plant Sciences Paris-Saclay, Université Paris-Saclay, Univ. Evry, INRAE, CNRS, 91405 Orsay, FranceInstitute of Plant Sciences Paris-Saclay, Université Paris-Saclay, Univ. Evry, INRAE, CNRS, 91405 Orsay, FranceInstitut Jean-Pierre Bourgin, INRAE, AgroParisTech, Université Paris-Saclay, 78000 Versailles, FranceBioscience and Laboratory of Molecular Biology, Wageningen University and Research, 6708PB Wageningen, The NetherlandsInstitute of Plant Sciences Paris-Saclay, Université Paris-Saclay, Univ. Evry, INRAE, CNRS, 91405 Orsay, FranceInstitute of Plant Sciences Paris-Saclay, Université Paris-Saclay, Univ. Evry, INRAE, CNRS, 91405 Orsay, FranceInstitute of Plant Sciences Paris-Saclay, Université Paris-Saclay, Univ. Evry, INRAE, CNRS, 91405 Orsay, FranceInstitut Jean-Pierre Bourgin, INRAE, AgroParisTech, Université Paris-Saclay, 78000 Versailles, FranceInstitute of Plant Sciences Paris-Saclay, Université Paris-Saclay, Univ. Evry, INRAE, CNRS, 91405 Orsay, FranceInstitut Jean-Pierre Bourgin, INRAE, AgroParisTech, Université Paris-Saclay, 78000 Versailles, FranceBioscience and Laboratory of Molecular Biology, Wageningen University and Research, 6708PB Wageningen, The NetherlandsDarwin21, Biological and Environmental Science and Engineering Division (BESE), King Abdullah University of Science and Technology, Thuwal 23955-6900, Saudi ArabiaInstitute of Plant Sciences Paris-Saclay, Université Paris-Saclay, Univ. Evry, INRAE, CNRS, 91405 Orsay, France<i>HEAT SHOCK FACTOR A2</i> (<i>HSFA2</i>) is a regulator of multiple environmental stress responses required for stress acclimation. We analyzed <i>HSFA2</i> co-regulated genes and identified 43 genes strongly co-regulated with <i>HSFA2</i> during multiple stresses. Motif enrichment analysis revealed an over-representation of the site II element (SIIE) in the promoters of these genes. In a yeast 1-hybrid screen with the SIIE, we identified the closely related R2R3-MYB transcription factors TT2 and MYB5. We found overexpression of <i>MYB5</i> or <i>TT2</i> rendered plants heat stress tolerant. In contrast, <i>tt2</i>, <i>myb5</i>, and <i>tt2/myb5</i> loss of function mutants showed heat stress hypersensitivity. Transient expression assays confirmed that MYB5 and TT2 can regulate the <i>HSFA2</i> promoter together with the other members of the MBW complex, TT8 and TRANSPARENT TESTA GLABRA 1 (TTG1) and that the SIIE was involved in this regulation. Transcriptomic analysis revealed that <i>TT2/MYB5</i> target promoters were enriched in SIIE. Overall, we report a new function of TT2 and MYB5 in stress resistance and a role in SIIE-mediated <i>HSFA2</i> regulation.https://www.mdpi.com/2073-4425/12/5/746environmental stressseed developmentsite II elementHSFA2TT2/ MYB5-MBW complex |
| spellingShingle | Pierre Jacob Gwilherm Brisou Marion Dalmais Johanne Thévenin Froukje van der Wal David Latrasse Ravi Suresh Devani Moussa Benhamed Bertrand Dubreucq Adnane Boualem Loic Lepiniec Richard G. H. Immink Heribert Hirt Abdelhafid Bendahmane The Seed Development Factors <i>TT2</i> and <i>MYB5</i> Regulate Heat Stress Response in <i>Arabidopsis</i> Genes environmental stress seed development site II element HSFA2 TT2/ MYB5-MBW complex |
| title | The Seed Development Factors <i>TT2</i> and <i>MYB5</i> Regulate Heat Stress Response in <i>Arabidopsis</i> |
| title_full | The Seed Development Factors <i>TT2</i> and <i>MYB5</i> Regulate Heat Stress Response in <i>Arabidopsis</i> |
| title_fullStr | The Seed Development Factors <i>TT2</i> and <i>MYB5</i> Regulate Heat Stress Response in <i>Arabidopsis</i> |
| title_full_unstemmed | The Seed Development Factors <i>TT2</i> and <i>MYB5</i> Regulate Heat Stress Response in <i>Arabidopsis</i> |
| title_short | The Seed Development Factors <i>TT2</i> and <i>MYB5</i> Regulate Heat Stress Response in <i>Arabidopsis</i> |
| title_sort | seed development factors i tt2 i and i myb5 i regulate heat stress response in i arabidopsis i |
| topic | environmental stress seed development site II element HSFA2 TT2/ MYB5-MBW complex |
| url | https://www.mdpi.com/2073-4425/12/5/746 |
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