The Separation, Purification, Structure Identification, and Antioxidant Activity of <i>Elaeagnus umbellata</i> Polysaccharides
In order to investigate the antioxidant activity of <i>Elaeagnus umbellata</i> polysaccharides, the physicochemical characteristics of purified <i>Elaeagnus umbellata</i> polysaccharides (EUP, consisting of two fractions, EUP1 and EUP2) were investigated using UV spectrophoto...
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MDPI AG
2023-09-01
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| Series: | Molecules |
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| Online Access: | https://www.mdpi.com/1420-3049/28/18/6468 |
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| author | Jinhua Zhang Xin Xu Xinyi Liu Min Chen Baoqing Bai Yukun Yang Tao Bo Sanhong Fan |
| author_facet | Jinhua Zhang Xin Xu Xinyi Liu Min Chen Baoqing Bai Yukun Yang Tao Bo Sanhong Fan |
| author_sort | Jinhua Zhang |
| collection | DOAJ |
| description | In order to investigate the antioxidant activity of <i>Elaeagnus umbellata</i> polysaccharides, the physicochemical characteristics of purified <i>Elaeagnus umbellata</i> polysaccharides (EUP, consisting of two fractions, EUP1 and EUP2) were investigated using UV spectrophotometry, high-performance liquid chromatography (HPLC), high-performance gel permeation chromatography (HPGPC), and Fourier transform infrared spectroscopy (FT-IR). This revealed that EUP1 and EUP2 were acidic polysaccharides with an average molecular weight (MW) of 63 and 38 kDa, respectively. EUP1 mainly consisted of L-rhamnose and D-galactose in a molar ratio of 2.05:1, and EUP2 consisted of D-mannose, L-rhamnose, D-galactose, and D-arabinose in a molar ratio of 2.06:1:2.78:1. Furthermore, EUP exhibited considerable antioxidant potential for scavenging hydroxyl, superoxide anion, DPPH, and ABTS radicals. Therefore, EUP can be developed as a potential antioxidant for the functional food or pharmaceutical field. |
| first_indexed | 2024-03-10T22:25:19Z |
| format | Article |
| id | doaj.art-a3a5e53f7c47487ea46bc4127ebebc70 |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-03-10T22:25:19Z |
| publishDate | 2023-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-a3a5e53f7c47487ea46bc4127ebebc702023-11-19T12:07:47ZengMDPI AGMolecules1420-30492023-09-012818646810.3390/molecules28186468The Separation, Purification, Structure Identification, and Antioxidant Activity of <i>Elaeagnus umbellata</i> PolysaccharidesJinhua Zhang0Xin Xu1Xinyi Liu2Min Chen3Baoqing Bai4Yukun Yang5Tao Bo6Sanhong Fan7School of Life Science, Shanxi University, Taiyuan 030006, ChinaSchool of Life Science, Shanxi University, Taiyuan 030006, ChinaSchool of Life Science, Shanxi University, Taiyuan 030006, ChinaShanxi Food Research Institute, Co., Ltd., Taiyuan 030024, ChinaSchool of Life Science, Shanxi University, Taiyuan 030006, ChinaSchool of Life Science, Shanxi University, Taiyuan 030006, ChinaInstitute of Biotechnology, Shanxi University, Taiyuan 030006, ChinaSchool of Life Science, Shanxi University, Taiyuan 030006, ChinaIn order to investigate the antioxidant activity of <i>Elaeagnus umbellata</i> polysaccharides, the physicochemical characteristics of purified <i>Elaeagnus umbellata</i> polysaccharides (EUP, consisting of two fractions, EUP1 and EUP2) were investigated using UV spectrophotometry, high-performance liquid chromatography (HPLC), high-performance gel permeation chromatography (HPGPC), and Fourier transform infrared spectroscopy (FT-IR). This revealed that EUP1 and EUP2 were acidic polysaccharides with an average molecular weight (MW) of 63 and 38 kDa, respectively. EUP1 mainly consisted of L-rhamnose and D-galactose in a molar ratio of 2.05:1, and EUP2 consisted of D-mannose, L-rhamnose, D-galactose, and D-arabinose in a molar ratio of 2.06:1:2.78:1. Furthermore, EUP exhibited considerable antioxidant potential for scavenging hydroxyl, superoxide anion, DPPH, and ABTS radicals. Therefore, EUP can be developed as a potential antioxidant for the functional food or pharmaceutical field.https://www.mdpi.com/1420-3049/28/18/6468<i>Elaeagnus umbellata</i>polysaccharidesantioxidant activityfunctional food |
| spellingShingle | Jinhua Zhang Xin Xu Xinyi Liu Min Chen Baoqing Bai Yukun Yang Tao Bo Sanhong Fan The Separation, Purification, Structure Identification, and Antioxidant Activity of <i>Elaeagnus umbellata</i> Polysaccharides Molecules <i>Elaeagnus umbellata</i> polysaccharides antioxidant activity functional food |
| title | The Separation, Purification, Structure Identification, and Antioxidant Activity of <i>Elaeagnus umbellata</i> Polysaccharides |
| title_full | The Separation, Purification, Structure Identification, and Antioxidant Activity of <i>Elaeagnus umbellata</i> Polysaccharides |
| title_fullStr | The Separation, Purification, Structure Identification, and Antioxidant Activity of <i>Elaeagnus umbellata</i> Polysaccharides |
| title_full_unstemmed | The Separation, Purification, Structure Identification, and Antioxidant Activity of <i>Elaeagnus umbellata</i> Polysaccharides |
| title_short | The Separation, Purification, Structure Identification, and Antioxidant Activity of <i>Elaeagnus umbellata</i> Polysaccharides |
| title_sort | separation purification structure identification and antioxidant activity of i elaeagnus umbellata i polysaccharides |
| topic | <i>Elaeagnus umbellata</i> polysaccharides antioxidant activity functional food |
| url | https://www.mdpi.com/1420-3049/28/18/6468 |
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