DNA methylation of selected tumor suppressor genes in endometrial hyperplasia
Aims. To investigate DNA methylation of specific gene promoters in endometrial hyperplasia compared to normal endometrial tissue. Materials and Methods. To search for epigenetic events, methylation-specific multiplex ligation-dependent probe amplification was employed to compare the methylation stat...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Palacký University Olomouc, Faculty of Medicine and Dentistry
2024-03-01
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| Series: | Biomedical Papers |
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| Online Access: | https://biomed.papers.upol.cz/artkey/bio-202401-0009_dna-methylation-of-selected-tumor-suppressor-genes-in-endometrial-hyperplasia.php |
| _version_ | 1797265200683941888 |
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| author | Ondrej Dvorak Munachiso Ndukwe Marcela Slavickova Jan Laco Jiri Spacek |
| author_facet | Ondrej Dvorak Munachiso Ndukwe Marcela Slavickova Jan Laco Jiri Spacek |
| author_sort | Ondrej Dvorak |
| collection | DOAJ |
| description | Aims. To investigate DNA methylation of specific gene promoters in endometrial hyperplasia compared to normal endometrial tissue. Materials and Methods. To search for epigenetic events, methylation-specific multiplex ligation-dependent probe amplification was employed to compare the methylation status of 64 tissue samples with atypical endometrial hyperplasia, 60 tissue samples with endometrial hyperplasia without atypia, and 40 control tissue samples with normal endometrium. Results. Differences in DNA methylation among the groups were found in PTEN, CDH13, and MSH6 promoters (PTEN: atypical hyperplasia 32%, benign hyperplasia 6.8%, normal endometrium 10%; P=0.004; CDH13: atypical hyperplasia, 50%; benign hyperplasia, 43%; normal endometrium 8.1%; P=0.003; MSH6 atypical hyperplasia 84%, benign hyperplasia, 62%; normal endometrium, 52%; P=0.008.) Higher rates of CDH13 promoter methylation were identified in the groups with both forms of endometrial hyperplasia when compared to the control group (atypical hyperplasia, P=0.003, benign hyperplasia, P=0.0002). A higher rate of DNA methylation of the PTEN and MSH6 promoters was observed in samples with atypical endometrial hyperplasia than in samples with benign endometrial hyperplasia (PTEN: P=0.02; MSH6: P=0.01) and samples with normal endometrial tissue (PTEN, P=0.04; MSH6, P=0.006). Conclusion. DNA methylation of CDH13, PTEN, and MSH6 appear to be involved in the development of endometrial hyperplasia. |
| first_indexed | 2024-04-25T00:41:01Z |
| format | Article |
| id | doaj.art-a3ae070db5d1468d9e96c6b5bfbb7f49 |
| institution | Directory Open Access Journal |
| issn | 1213-8118 1804-7521 |
| language | English |
| last_indexed | 2024-04-25T00:41:01Z |
| publishDate | 2024-03-01 |
| publisher | Palacký University Olomouc, Faculty of Medicine and Dentistry |
| record_format | Article |
| series | Biomedical Papers |
| spelling | doaj.art-a3ae070db5d1468d9e96c6b5bfbb7f492024-03-12T09:46:45ZengPalacký University Olomouc, Faculty of Medicine and DentistryBiomedical Papers1213-81181804-75212024-03-011681687310.5507/bp.2022.053bio-202401-0009DNA methylation of selected tumor suppressor genes in endometrial hyperplasiaOndrej Dvorak0Munachiso Ndukwe1Marcela Slavickova2Jan Laco3Jiri Spacek4Department of Obstetrics and Gynecology, University Hospital Hradec Kralove and Faculty of Medicine, Charles University, Hradec Kralove, Czech RepublicDepartment of Obstetrics and Gynecology, University Hospital Hradec Kralove and Faculty of Medicine, Charles University, Hradec Kralove, Czech RepublicDepartment of Clinical Biochemistry and Diagnostics and Osteocenter, University Hospital Hradec Kralove and Faculty of Medicine, Charles University, Hradec Kralove, Czech RepublicThe Fingerland Department of Pathology, University Hospital Hradec Kralove and Faculty of Medicine, Charles University, Hradec Kralove, Czech RepublicDepartment of Obstetrics and Gynecology, University Hospital Hradec Kralove and Faculty of Medicine, Charles University, Hradec Kralove, Czech RepublicAims. To investigate DNA methylation of specific gene promoters in endometrial hyperplasia compared to normal endometrial tissue. Materials and Methods. To search for epigenetic events, methylation-specific multiplex ligation-dependent probe amplification was employed to compare the methylation status of 64 tissue samples with atypical endometrial hyperplasia, 60 tissue samples with endometrial hyperplasia without atypia, and 40 control tissue samples with normal endometrium. Results. Differences in DNA methylation among the groups were found in PTEN, CDH13, and MSH6 promoters (PTEN: atypical hyperplasia 32%, benign hyperplasia 6.8%, normal endometrium 10%; P=0.004; CDH13: atypical hyperplasia, 50%; benign hyperplasia, 43%; normal endometrium 8.1%; P=0.003; MSH6 atypical hyperplasia 84%, benign hyperplasia, 62%; normal endometrium, 52%; P=0.008.) Higher rates of CDH13 promoter methylation were identified in the groups with both forms of endometrial hyperplasia when compared to the control group (atypical hyperplasia, P=0.003, benign hyperplasia, P=0.0002). A higher rate of DNA methylation of the PTEN and MSH6 promoters was observed in samples with atypical endometrial hyperplasia than in samples with benign endometrial hyperplasia (PTEN: P=0.02; MSH6: P=0.01) and samples with normal endometrial tissue (PTEN, P=0.04; MSH6, P=0.006). Conclusion. DNA methylation of CDH13, PTEN, and MSH6 appear to be involved in the development of endometrial hyperplasia.https://biomed.papers.upol.cz/artkey/bio-202401-0009_dna-methylation-of-selected-tumor-suppressor-genes-in-endometrial-hyperplasia.phpmethylationcdh13ptenmsh6endometrial hyperplasiaepigenetics |
| spellingShingle | Ondrej Dvorak Munachiso Ndukwe Marcela Slavickova Jan Laco Jiri Spacek DNA methylation of selected tumor suppressor genes in endometrial hyperplasia Biomedical Papers methylation cdh13 pten msh6 endometrial hyperplasia epigenetics |
| title | DNA methylation of selected tumor suppressor genes in endometrial hyperplasia |
| title_full | DNA methylation of selected tumor suppressor genes in endometrial hyperplasia |
| title_fullStr | DNA methylation of selected tumor suppressor genes in endometrial hyperplasia |
| title_full_unstemmed | DNA methylation of selected tumor suppressor genes in endometrial hyperplasia |
| title_short | DNA methylation of selected tumor suppressor genes in endometrial hyperplasia |
| title_sort | dna methylation of selected tumor suppressor genes in endometrial hyperplasia |
| topic | methylation cdh13 pten msh6 endometrial hyperplasia epigenetics |
| url | https://biomed.papers.upol.cz/artkey/bio-202401-0009_dna-methylation-of-selected-tumor-suppressor-genes-in-endometrial-hyperplasia.php |
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