Male C57BL6/N and C57BL6/J Mice Respond Differently to Constant Light and Running-Wheel Access
Previous studies have shown that exposure to circadian disruption produces negative effects on overall health and behavior. More recent studies illustrate that strain differences in the behavioral and physiological responses to circadian disruption exist, even if the strains have similar genetic bac...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2019-12-01
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| Series: | Frontiers in Behavioral Neuroscience |
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| Online Access: | https://www.frontiersin.org/article/10.3389/fnbeh.2019.00268/full |
| _version_ | 1811197780708818944 |
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| author | Kimberly M. Capri Kimberly M. Capri Marissa J. Maroni Marissa J. Maroni Hannah V. Deane Holly A. Concepcion Holly DeCourcey Ryan W. Logan Ryan W. Logan Joseph A. Seggio |
| author_facet | Kimberly M. Capri Kimberly M. Capri Marissa J. Maroni Marissa J. Maroni Hannah V. Deane Holly A. Concepcion Holly DeCourcey Ryan W. Logan Ryan W. Logan Joseph A. Seggio |
| author_sort | Kimberly M. Capri |
| collection | DOAJ |
| description | Previous studies have shown that exposure to circadian disruption produces negative effects on overall health and behavior. More recent studies illustrate that strain differences in the behavioral and physiological responses to circadian disruption exist, even if the strains have similar genetic backgrounds. As such, we investigated the effects of constant room-level light (LL) with running-wheel access on the behavior and physiology of male C57BL6/J from Jackson Laboratories and C57BL6/N from Charles River Laboratories mice. Mice were exposed to either a 12:12 light-dark (LD) cycle or LL and given either a standard home cage or a cage with a running-wheel. Following 6 weeks of LD or LL, their response to behavioral assays (open-field, light-dark box, novel object) and measures of metabolism were observed. Under standard LD, C57BL6/J mice exhibited increased locomotor activity and reduced exploratory behavior compared to C57BL6/N mice. In LL, C57BL6/J mice had greater period lengthening and increased anxiety, while C57BL6/N mice exhibited increased weight gain and no change in exploratory behavior. C57BL6/J mice also decreased exploration with running-wheel access while C57BL6/N mice did not. These results further demonstrate that C57BL/6 substrains exhibit different behavioral and physiological responses to circadian disruption and wheel-running access. |
| first_indexed | 2024-04-12T01:19:55Z |
| format | Article |
| id | doaj.art-a3afb2f5061842b995480b52c8bd129a |
| institution | Directory Open Access Journal |
| issn | 1662-5153 |
| language | English |
| last_indexed | 2024-04-12T01:19:55Z |
| publishDate | 2019-12-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Behavioral Neuroscience |
| spelling | doaj.art-a3afb2f5061842b995480b52c8bd129a2022-12-22T03:53:50ZengFrontiers Media S.A.Frontiers in Behavioral Neuroscience1662-51532019-12-011310.3389/fnbeh.2019.00268497935Male C57BL6/N and C57BL6/J Mice Respond Differently to Constant Light and Running-Wheel AccessKimberly M. Capri0Kimberly M. Capri1Marissa J. Maroni2Marissa J. Maroni3Hannah V. Deane4Holly A. Concepcion5Holly DeCourcey6Ryan W. Logan7Ryan W. Logan8Joseph A. Seggio9Department of Biological Sciences, Bridgewater State University, Bridgewater, MA, United StatesDepartment of Mathematics and Statistics, Boston University, Boston, MA, United StatesDepartment of Biological Sciences, Bridgewater State University, Bridgewater, MA, United StatesPerelman School of Medicine, University of Pennsylvania, Philadelphia, PA, United StatesDepartment of Biological Sciences, Bridgewater State University, Bridgewater, MA, United StatesDepartment of Biological Sciences, Bridgewater State University, Bridgewater, MA, United StatesDepartment of Biological Sciences, Bridgewater State University, Bridgewater, MA, United StatesTranslational Neuroscience Program, Department of Psychiatry, University of Pittsburgh School of Medicine, Pittsburgh, PA, United StatesCenter for Systems Neurogenetics of Addiction, The Jackson Laboratory, Bar Harbor, ME, United StatesDepartment of Biological Sciences, Bridgewater State University, Bridgewater, MA, United StatesPrevious studies have shown that exposure to circadian disruption produces negative effects on overall health and behavior. More recent studies illustrate that strain differences in the behavioral and physiological responses to circadian disruption exist, even if the strains have similar genetic backgrounds. As such, we investigated the effects of constant room-level light (LL) with running-wheel access on the behavior and physiology of male C57BL6/J from Jackson Laboratories and C57BL6/N from Charles River Laboratories mice. Mice were exposed to either a 12:12 light-dark (LD) cycle or LL and given either a standard home cage or a cage with a running-wheel. Following 6 weeks of LD or LL, their response to behavioral assays (open-field, light-dark box, novel object) and measures of metabolism were observed. Under standard LD, C57BL6/J mice exhibited increased locomotor activity and reduced exploratory behavior compared to C57BL6/N mice. In LL, C57BL6/J mice had greater period lengthening and increased anxiety, while C57BL6/N mice exhibited increased weight gain and no change in exploratory behavior. C57BL6/J mice also decreased exploration with running-wheel access while C57BL6/N mice did not. These results further demonstrate that C57BL/6 substrains exhibit different behavioral and physiological responses to circadian disruption and wheel-running access.https://www.frontiersin.org/article/10.3389/fnbeh.2019.00268/fullmouse modelcircadian rhythmstrain differencemiceconstant lightrunning wheel |
| spellingShingle | Kimberly M. Capri Kimberly M. Capri Marissa J. Maroni Marissa J. Maroni Hannah V. Deane Holly A. Concepcion Holly DeCourcey Ryan W. Logan Ryan W. Logan Joseph A. Seggio Male C57BL6/N and C57BL6/J Mice Respond Differently to Constant Light and Running-Wheel Access Frontiers in Behavioral Neuroscience mouse model circadian rhythm strain difference mice constant light running wheel |
| title | Male C57BL6/N and C57BL6/J Mice Respond Differently to Constant Light and Running-Wheel Access |
| title_full | Male C57BL6/N and C57BL6/J Mice Respond Differently to Constant Light and Running-Wheel Access |
| title_fullStr | Male C57BL6/N and C57BL6/J Mice Respond Differently to Constant Light and Running-Wheel Access |
| title_full_unstemmed | Male C57BL6/N and C57BL6/J Mice Respond Differently to Constant Light and Running-Wheel Access |
| title_short | Male C57BL6/N and C57BL6/J Mice Respond Differently to Constant Light and Running-Wheel Access |
| title_sort | male c57bl6 n and c57bl6 j mice respond differently to constant light and running wheel access |
| topic | mouse model circadian rhythm strain difference mice constant light running wheel |
| url | https://www.frontiersin.org/article/10.3389/fnbeh.2019.00268/full |
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