The kinase LYK5 is a major chitin receptor in Arabidopsis and forms a chitin-induced complex with related kinase CERK1
Chitin is a fungal microbe-associated molecular pattern recognized in Arabidopsis by a lysin motif receptor kinase (LYK), AtCERK1. Previous research suggested that AtCERK1 is the major chitin receptor and mediates chitin-induced signaling through homodimerization and phosphorylation. However, the re...
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| Format: | Article |
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eLife Sciences Publications Ltd
2014-10-01
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| Series: | eLife |
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| Online Access: | https://elifesciences.org/articles/03766 |
| _version_ | 1818028333773881344 |
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| author | Yangrong Cao Yan Liang Kiwamu Tanaka Cuong T Nguyen Robert P Jedrzejczak Andrzej Joachimiak Gary Stacey |
| author_facet | Yangrong Cao Yan Liang Kiwamu Tanaka Cuong T Nguyen Robert P Jedrzejczak Andrzej Joachimiak Gary Stacey |
| author_sort | Yangrong Cao |
| collection | DOAJ |
| description | Chitin is a fungal microbe-associated molecular pattern recognized in Arabidopsis by a lysin motif receptor kinase (LYK), AtCERK1. Previous research suggested that AtCERK1 is the major chitin receptor and mediates chitin-induced signaling through homodimerization and phosphorylation. However, the reported chitin binding affinity of AtCERK1 is quite low, suggesting another receptor with high chitin binding affinity might be present. Here, we propose that AtLYK5 is the primary chitin receptor in Arabidopsis. Mutations in AtLYK5 resulted in a significant reduction in chitin response. However, AtLYK5 shares overlapping function with AtLYK4 and, therefore, Atlyk4/Atlyk5-2 double mutants show a complete loss of chitin response. AtLYK5 interacts with AtCERK1 in a chitin-dependent manner. Chitin binding to AtLYK5 is indispensable for chitin-induced AtCERK1 phosphorylation. AtLYK5 binds chitin at a much higher affinity than AtCERK1. The data suggest that AtLYK5 is the primary receptor for chitin, forming a chitin inducible complex with AtCERK1 to induce plant immunity. |
| first_indexed | 2024-12-10T05:02:08Z |
| format | Article |
| id | doaj.art-a3b078a8ec344818b1a7ae62a358525d |
| institution | Directory Open Access Journal |
| issn | 2050-084X |
| language | English |
| last_indexed | 2024-12-10T05:02:08Z |
| publishDate | 2014-10-01 |
| publisher | eLife Sciences Publications Ltd |
| record_format | Article |
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| spelling | doaj.art-a3b078a8ec344818b1a7ae62a358525d2022-12-22T02:01:22ZengeLife Sciences Publications LtdeLife2050-084X2014-10-01310.7554/eLife.03766The kinase LYK5 is a major chitin receptor in Arabidopsis and forms a chitin-induced complex with related kinase CERK1Yangrong Cao0Yan Liang1Kiwamu Tanaka2https://orcid.org/0000-0001-5045-560XCuong T Nguyen3Robert P Jedrzejczak4Andrzej Joachimiak5Gary Stacey6Division of Plant Sciences, National Center for Soybean Biotechnology, University of Missouri, Columbia, United States; Department of Biochemistry, University of Missouri, Columbia, United StatesDivision of Plant Sciences, National Center for Soybean Biotechnology, University of Missouri, Columbia, United States; Department of Biochemistry, University of Missouri, Columbia, United StatesDivision of Plant Sciences, National Center for Soybean Biotechnology, University of Missouri, Columbia, United States; Department of Biochemistry, University of Missouri, Columbia, United StatesDivision of Plant Sciences, National Center for Soybean Biotechnology, University of Missouri, Columbia, United States; Department of Biochemistry, University of Missouri, Columbia, United StatesMidwest Center for Structural Genomics, Argonne National Laboratory, Argonne, United StatesMidwest Center for Structural Genomics, Argonne National Laboratory, Argonne, United StatesDivision of Plant Sciences, National Center for Soybean Biotechnology, University of Missouri, Columbia, United States; Department of Biochemistry, University of Missouri, Columbia, United StatesChitin is a fungal microbe-associated molecular pattern recognized in Arabidopsis by a lysin motif receptor kinase (LYK), AtCERK1. Previous research suggested that AtCERK1 is the major chitin receptor and mediates chitin-induced signaling through homodimerization and phosphorylation. However, the reported chitin binding affinity of AtCERK1 is quite low, suggesting another receptor with high chitin binding affinity might be present. Here, we propose that AtLYK5 is the primary chitin receptor in Arabidopsis. Mutations in AtLYK5 resulted in a significant reduction in chitin response. However, AtLYK5 shares overlapping function with AtLYK4 and, therefore, Atlyk4/Atlyk5-2 double mutants show a complete loss of chitin response. AtLYK5 interacts with AtCERK1 in a chitin-dependent manner. Chitin binding to AtLYK5 is indispensable for chitin-induced AtCERK1 phosphorylation. AtLYK5 binds chitin at a much higher affinity than AtCERK1. The data suggest that AtLYK5 is the primary receptor for chitin, forming a chitin inducible complex with AtCERK1 to induce plant immunity.https://elifesciences.org/articles/03766Arabidoposisplant innate immunitychitin receptorCERK1LYK5 |
| spellingShingle | Yangrong Cao Yan Liang Kiwamu Tanaka Cuong T Nguyen Robert P Jedrzejczak Andrzej Joachimiak Gary Stacey The kinase LYK5 is a major chitin receptor in Arabidopsis and forms a chitin-induced complex with related kinase CERK1 eLife Arabidoposis plant innate immunity chitin receptor CERK1 LYK5 |
| title | The kinase LYK5 is a major chitin receptor in Arabidopsis and forms a chitin-induced complex with related kinase CERK1 |
| title_full | The kinase LYK5 is a major chitin receptor in Arabidopsis and forms a chitin-induced complex with related kinase CERK1 |
| title_fullStr | The kinase LYK5 is a major chitin receptor in Arabidopsis and forms a chitin-induced complex with related kinase CERK1 |
| title_full_unstemmed | The kinase LYK5 is a major chitin receptor in Arabidopsis and forms a chitin-induced complex with related kinase CERK1 |
| title_short | The kinase LYK5 is a major chitin receptor in Arabidopsis and forms a chitin-induced complex with related kinase CERK1 |
| title_sort | kinase lyk5 is a major chitin receptor in arabidopsis and forms a chitin induced complex with related kinase cerk1 |
| topic | Arabidoposis plant innate immunity chitin receptor CERK1 LYK5 |
| url | https://elifesciences.org/articles/03766 |
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