Microbiological profile of blood stream infection: Genetic relatedness of carbapenemase producing klebsiella Pneumoniae isolates using ERIC PCR
Background: There has been an expansion in antibiotic resistance among Enterobacteriaceae over the previous ten years raising serious questions about global health. Our objectives were to isolate the carbapenem-resistant Gram-negative bacilli that cause blood stream infections and investigate the ge...
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| Format: | Article |
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Zagazig University, Faculty of Medicine
2023-02-01
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| Series: | Microbes and Infectious Diseases |
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| Online Access: | https://mid.journals.ekb.eg/article_275006_925c8cc6d3065e9695cc4f653b78d25b.pdf |
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| author | Marwa Taha Sarah Shouib Walid ELmonir Shaima Zahra Eman Hegazy |
| author_facet | Marwa Taha Sarah Shouib Walid ELmonir Shaima Zahra Eman Hegazy |
| author_sort | Marwa Taha |
| collection | DOAJ |
| description | Background: There has been an expansion in antibiotic resistance among Enterobacteriaceae over the previous ten years raising serious questions about global health. Our objectives were to isolate the carbapenem-resistant Gram-negative bacilli that cause blood stream infections and investigate the genetic relatedness between carbapenemase producingKlebsiella pneumoniae (K. pneumoniae) isolates. Methods: A total of 255 blood specimens were withdrawn into blood culture bottles and incubated, pathogenic microorganisms were identified, antibiotic susceptibility was done for Gram-negative isolates. Carbapenemase producers were screened for molecular detection of carbapenemase and extended spectrum β-lactamase genes. Results: One hundred and thirty-three (133) pathogens were isolated, of these 77 Gram-negative isolates (including 32 K. pneumoniae,18 E. coli, 12 Acinetobacter, 9 Pseudomonas and 6 Enterobacter cloacae). Out of them, 55 were carbapenem resistant by phenotypic method. 23/55 of them were harboring carbapenemase genes by PCR ERIC, results showed that Anesthesia intensive care unit (ICU) isolates showed >90% genetic similarity while isolates from Chest and Internal medicine ICUs showed <70% similarity. Conclusion: Increased awareness, persistent observation, and strict adherence to the recommendations for antibiotic stewardship should be used to limit spread of carbapenem resistant Gram-negative bacilli. |
| first_indexed | 2024-04-10T18:39:49Z |
| format | Article |
| id | doaj.art-a3b353e47c2a44bc900b78aa3fefc3f6 |
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| issn | 2682-4132 2682-4140 |
| language | English |
| last_indexed | 2024-04-10T18:39:49Z |
| publishDate | 2023-02-01 |
| publisher | Zagazig University, Faculty of Medicine |
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| series | Microbes and Infectious Diseases |
| spelling | doaj.art-a3b353e47c2a44bc900b78aa3fefc3f62023-02-01T17:44:12ZengZagazig University, Faculty of MedicineMicrobes and Infectious Diseases2682-41322682-41402023-02-014116818110.21608/mid.2022.175107.1414275006Microbiological profile of blood stream infection: Genetic relatedness of carbapenemase producing klebsiella Pneumoniae isolates using ERIC PCRMarwa Taha0Sarah Shouib1Walid ELmonir2Shaima Zahra3Eman Hegazy4Department of Medical Microbiology and Immunology, Faculty of Medicine, Tanta University, Egypt.Department of Clinical Pathology, Faculty of Medicine, Tanta University, Egypt.Department of Hygiene and Preventive Medicine (Zoonoses), Faculty of Veterinary Medicine, Kafr Elsheikh University, Egypt.Department of Anesthesiology, Surgical Intensive Care and Pain Medicine, Faculty of Medicine, Tanta University, Egypt.Department of Medical Microbiology and Immunology, Faculty of Medicine, Tanta University, Egypt.Background: There has been an expansion in antibiotic resistance among Enterobacteriaceae over the previous ten years raising serious questions about global health. Our objectives were to isolate the carbapenem-resistant Gram-negative bacilli that cause blood stream infections and investigate the genetic relatedness between carbapenemase producingKlebsiella pneumoniae (K. pneumoniae) isolates. Methods: A total of 255 blood specimens were withdrawn into blood culture bottles and incubated, pathogenic microorganisms were identified, antibiotic susceptibility was done for Gram-negative isolates. Carbapenemase producers were screened for molecular detection of carbapenemase and extended spectrum β-lactamase genes. Results: One hundred and thirty-three (133) pathogens were isolated, of these 77 Gram-negative isolates (including 32 K. pneumoniae,18 E. coli, 12 Acinetobacter, 9 Pseudomonas and 6 Enterobacter cloacae). Out of them, 55 were carbapenem resistant by phenotypic method. 23/55 of them were harboring carbapenemase genes by PCR ERIC, results showed that Anesthesia intensive care unit (ICU) isolates showed >90% genetic similarity while isolates from Chest and Internal medicine ICUs showed <70% similarity. Conclusion: Increased awareness, persistent observation, and strict adherence to the recommendations for antibiotic stewardship should be used to limit spread of carbapenem resistant Gram-negative bacilli.https://mid.journals.ekb.eg/article_275006_925c8cc6d3065e9695cc4f653b78d25b.pdfbsisk. pneumoniaeeric pcrcarbapenem resistant gram-negative bacilli |
| spellingShingle | Marwa Taha Sarah Shouib Walid ELmonir Shaima Zahra Eman Hegazy Microbiological profile of blood stream infection: Genetic relatedness of carbapenemase producing klebsiella Pneumoniae isolates using ERIC PCR Microbes and Infectious Diseases bsis k. pneumoniae eric pcr carbapenem resistant gram-negative bacilli |
| title | Microbiological profile of blood stream infection: Genetic relatedness of carbapenemase producing klebsiella Pneumoniae isolates using ERIC PCR |
| title_full | Microbiological profile of blood stream infection: Genetic relatedness of carbapenemase producing klebsiella Pneumoniae isolates using ERIC PCR |
| title_fullStr | Microbiological profile of blood stream infection: Genetic relatedness of carbapenemase producing klebsiella Pneumoniae isolates using ERIC PCR |
| title_full_unstemmed | Microbiological profile of blood stream infection: Genetic relatedness of carbapenemase producing klebsiella Pneumoniae isolates using ERIC PCR |
| title_short | Microbiological profile of blood stream infection: Genetic relatedness of carbapenemase producing klebsiella Pneumoniae isolates using ERIC PCR |
| title_sort | microbiological profile of blood stream infection genetic relatedness of carbapenemase producing klebsiella pneumoniae isolates using eric pcr |
| topic | bsis k. pneumoniae eric pcr carbapenem resistant gram-negative bacilli |
| url | https://mid.journals.ekb.eg/article_275006_925c8cc6d3065e9695cc4f653b78d25b.pdf |
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