Protective effects of melatonin in cisplatin-induced cardiac toxicity: possible role of BDNF-TNF-α signaling pathway
ABSTRACT Purpose: The present study explored the role of melatonin in cisplatin-induced cardiac injury along with the possible role of brain-derived neurotrophic factor (BDNF) in melatonin-mediated effects. Methods: Wistar rats were administered cisplatin (10 mg/kg), and cardiac injury was assesse...
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| Format: | Article |
| Language: | English |
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Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia
2022-05-01
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| Series: | Acta Cirúrgica Brasileira |
| Subjects: | |
| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-86502022000200207&lng=en&tlng=en |
| _version_ | 1818186875023654912 |
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| author | Xiaoqing Zhuo Honglei Jiang |
| author_facet | Xiaoqing Zhuo Honglei Jiang |
| author_sort | Xiaoqing Zhuo |
| collection | DOAJ |
| description | ABSTRACT Purpose: The present study explored the role of melatonin in cisplatin-induced cardiac injury along with the possible role of brain-derived neurotrophic factor (BDNF) in melatonin-mediated effects. Methods: Wistar rats were administered cisplatin (10 mg/kg), and cardiac injury was assessed by measuring the levels of cardiac troponin (cTnT) and lactate dehydrogenase (LDH-1).The extent of apoptosis was measured by measuring caspase-3 (pro-apoptotic) and Bcl-2 (anti-apoptotic) in hearts. The levels of BDNF, tumour necrosis factor α (TNF-α) and reduced glutathione were measured in heart. Melatonin (5 and 10 mg/kg) was administered for 15 days, and the role of BDNF was identified by co-administering BDNF inhibitor, ANA-12 (0.25 and 0.5 mg/kg). Results: Melatonin attenuated cTnT and LDH-1 levels along with reduction in caspase-3 and increase in Bcl-2. It also increased cisplatin-induced decrease in BDNF, increase in TNF-α and decrease in reduced glutathione levels. Moreover, ANA-12 abolished the cardioprotective effects, anti-inflammatory and antioxidant effects of melatonin suggesting the role of BDNF in melatonin-mediated effects in cisplatin-induced cardiac injury. Conclusions: Melatonin is useful in cisplatin-induced cardiac injury, which may be due to an increase in BDNF, decrease in inflammation and increase in antioxidant activities. |
| first_indexed | 2024-12-11T23:02:05Z |
| format | Article |
| id | doaj.art-a3b4a731d21f4ef38e7637c286b61a4d |
| institution | Directory Open Access Journal |
| issn | 1678-2674 |
| language | English |
| last_indexed | 2024-12-11T23:02:05Z |
| publishDate | 2022-05-01 |
| publisher | Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia |
| record_format | Article |
| series | Acta Cirúrgica Brasileira |
| spelling | doaj.art-a3b4a731d21f4ef38e7637c286b61a4d2022-12-22T00:47:02ZengSociedade Brasileira para o Desenvolvimento da Pesquisa em CirurgiaActa Cirúrgica Brasileira1678-26742022-05-0137210.1590/acb370208Protective effects of melatonin in cisplatin-induced cardiac toxicity: possible role of BDNF-TNF-α signaling pathwayXiaoqing Zhuohttps://orcid.org/0000-0003-4784-9661Honglei Jianghttps://orcid.org/0000-0003-3791-5688ABSTRACT Purpose: The present study explored the role of melatonin in cisplatin-induced cardiac injury along with the possible role of brain-derived neurotrophic factor (BDNF) in melatonin-mediated effects. Methods: Wistar rats were administered cisplatin (10 mg/kg), and cardiac injury was assessed by measuring the levels of cardiac troponin (cTnT) and lactate dehydrogenase (LDH-1).The extent of apoptosis was measured by measuring caspase-3 (pro-apoptotic) and Bcl-2 (anti-apoptotic) in hearts. The levels of BDNF, tumour necrosis factor α (TNF-α) and reduced glutathione were measured in heart. Melatonin (5 and 10 mg/kg) was administered for 15 days, and the role of BDNF was identified by co-administering BDNF inhibitor, ANA-12 (0.25 and 0.5 mg/kg). Results: Melatonin attenuated cTnT and LDH-1 levels along with reduction in caspase-3 and increase in Bcl-2. It also increased cisplatin-induced decrease in BDNF, increase in TNF-α and decrease in reduced glutathione levels. Moreover, ANA-12 abolished the cardioprotective effects, anti-inflammatory and antioxidant effects of melatonin suggesting the role of BDNF in melatonin-mediated effects in cisplatin-induced cardiac injury. Conclusions: Melatonin is useful in cisplatin-induced cardiac injury, which may be due to an increase in BDNF, decrease in inflammation and increase in antioxidant activities.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-86502022000200207&lng=en&tlng=enMelatoninCisplatinGlutathioneBrain-Derived Neurotrophic FactorRats |
| spellingShingle | Xiaoqing Zhuo Honglei Jiang Protective effects of melatonin in cisplatin-induced cardiac toxicity: possible role of BDNF-TNF-α signaling pathway Acta Cirúrgica Brasileira Melatonin Cisplatin Glutathione Brain-Derived Neurotrophic Factor Rats |
| title | Protective effects of melatonin in cisplatin-induced cardiac toxicity: possible role of BDNF-TNF-α signaling pathway |
| title_full | Protective effects of melatonin in cisplatin-induced cardiac toxicity: possible role of BDNF-TNF-α signaling pathway |
| title_fullStr | Protective effects of melatonin in cisplatin-induced cardiac toxicity: possible role of BDNF-TNF-α signaling pathway |
| title_full_unstemmed | Protective effects of melatonin in cisplatin-induced cardiac toxicity: possible role of BDNF-TNF-α signaling pathway |
| title_short | Protective effects of melatonin in cisplatin-induced cardiac toxicity: possible role of BDNF-TNF-α signaling pathway |
| title_sort | protective effects of melatonin in cisplatin induced cardiac toxicity possible role of bdnf tnf α signaling pathway |
| topic | Melatonin Cisplatin Glutathione Brain-Derived Neurotrophic Factor Rats |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-86502022000200207&lng=en&tlng=en |
| work_keys_str_mv | AT xiaoqingzhuo protectiveeffectsofmelatoninincisplatininducedcardiactoxicitypossibleroleofbdnftnfasignalingpathway AT hongleijiang protectiveeffectsofmelatoninincisplatininducedcardiactoxicitypossibleroleofbdnftnfasignalingpathway |