Effect of methotrexate on neurobehavior and cholinesterase in chicks
This reading aimed to determine the therapeutic index of methotrexate (MTX) with a note on the consequence of methotrexate in chicks on neurobehavioral in addition to calculating the activity for cholinesterase in plasma and brain in chicks. Two groups with 10 chicks each were utilized once. The unt...
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| Format: | Article |
| Language: | Arabic |
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University of Mosul, College of Veterinary Medicine
2023-10-01
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| Series: | Iraqi Journal of Veterinary Sciences |
| Subjects: | |
| Online Access: | https://www.vetmedmosul.com/article_180625_a4d47c7eb644e06551b0c7d638c86a0c.pdf |
| _version_ | 1797669189615353856 |
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| author | Maab A. Fadel Khayrea A. Mustafa Imad A. Thanoon |
| author_facet | Maab A. Fadel Khayrea A. Mustafa Imad A. Thanoon |
| author_sort | Maab A. Fadel |
| collection | DOAJ |
| description | This reading aimed to determine the therapeutic index of methotrexate (MTX) with a note on the consequence of methotrexate in chicks on neurobehavioral in addition to calculating the activity for cholinesterase in plasma and brain in chicks. Two groups with 10 chicks each were utilized once. The untreated and methotrexate groups were given saline and MTX at dose 40 mg/kg for 5 days, respectively. The methotrexate group obtained considerable amounts of methotrexate 9th day of the start of the experiment for five days. All chicks were exposed to open-field activity and tonic immobility tests. Brain tissue with blood samples were collected to evaluate cholinesterase levels in chicks. ED50 and LD50 for methotrexate in the chicks were 14.36mg/ kg and102.63 mg/ kg, correspondingly. In addition, Therapeutic index of methotrexate was 7.15. The methotrexate group explained an important enhance in latency to move and tonic immobility evaluated with an untreated group, there is a significant decline in lines crossed, and escape jumps compared with untreated group, and the cholinesterase activity showed a significantly reduced in plasma and brain samples treated with methotrexate and inhibition expressed significantly increased evaluated through the control group. Our findings conclude that methotrexate has a depressant action on the nervous structure and an inhibitory action on the cholinesterase level in the chick’s model. |
| first_indexed | 2024-03-11T20:40:32Z |
| format | Article |
| id | doaj.art-a3b510dab48c40048eea8ee9969a46b1 |
| institution | Directory Open Access Journal |
| issn | 1607-3894 2071-1255 |
| language | Arabic |
| last_indexed | 2024-03-11T20:40:32Z |
| publishDate | 2023-10-01 |
| publisher | University of Mosul, College of Veterinary Medicine |
| record_format | Article |
| series | Iraqi Journal of Veterinary Sciences |
| spelling | doaj.art-a3b510dab48c40048eea8ee9969a46b12023-10-02T03:07:56ZaraUniversity of Mosul, College of Veterinary MedicineIraqi Journal of Veterinary Sciences1607-38942071-12552023-10-0137498598910.33899/ijvs.2023.138804.2844180625Effect of methotrexate on neurobehavior and cholinesterase in chicksMaab A. Fadel0Khayrea A. Mustafa1Imad A. Thanoon2Department of Physiology, Chemistry and Pharmacology, College of Veterinary Medicine, University of Mosul, Mosul, IraqDepartment of Physiology, Biochemistry, and Pharmacology, College of Veterinary Medicine, University of Mosul, Mosul, IraqDepartment of Pharmacology, College of Medicine, University of Mosul, Mosul, IraqThis reading aimed to determine the therapeutic index of methotrexate (MTX) with a note on the consequence of methotrexate in chicks on neurobehavioral in addition to calculating the activity for cholinesterase in plasma and brain in chicks. Two groups with 10 chicks each were utilized once. The untreated and methotrexate groups were given saline and MTX at dose 40 mg/kg for 5 days, respectively. The methotrexate group obtained considerable amounts of methotrexate 9th day of the start of the experiment for five days. All chicks were exposed to open-field activity and tonic immobility tests. Brain tissue with blood samples were collected to evaluate cholinesterase levels in chicks. ED50 and LD50 for methotrexate in the chicks were 14.36mg/ kg and102.63 mg/ kg, correspondingly. In addition, Therapeutic index of methotrexate was 7.15. The methotrexate group explained an important enhance in latency to move and tonic immobility evaluated with an untreated group, there is a significant decline in lines crossed, and escape jumps compared with untreated group, and the cholinesterase activity showed a significantly reduced in plasma and brain samples treated with methotrexate and inhibition expressed significantly increased evaluated through the control group. Our findings conclude that methotrexate has a depressant action on the nervous structure and an inhibitory action on the cholinesterase level in the chick’s model.https://www.vetmedmosul.com/article_180625_a4d47c7eb644e06551b0c7d638c86a0c.pdftherapeutic indexcholinesterasemedian effective dosemethotrexatechicks |
| spellingShingle | Maab A. Fadel Khayrea A. Mustafa Imad A. Thanoon Effect of methotrexate on neurobehavior and cholinesterase in chicks Iraqi Journal of Veterinary Sciences therapeutic index cholinesterase median effective dose methotrexate chicks |
| title | Effect of methotrexate on neurobehavior and cholinesterase in chicks |
| title_full | Effect of methotrexate on neurobehavior and cholinesterase in chicks |
| title_fullStr | Effect of methotrexate on neurobehavior and cholinesterase in chicks |
| title_full_unstemmed | Effect of methotrexate on neurobehavior and cholinesterase in chicks |
| title_short | Effect of methotrexate on neurobehavior and cholinesterase in chicks |
| title_sort | effect of methotrexate on neurobehavior and cholinesterase in chicks |
| topic | therapeutic index cholinesterase median effective dose methotrexate chicks |
| url | https://www.vetmedmosul.com/article_180625_a4d47c7eb644e06551b0c7d638c86a0c.pdf |
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