Experimental Infection of Different Tomato Genotypes with Tomato mosaic virus Led to a Low Viral Population Heterogeneity in the Capsid Protein Encoding Region
The complete genome sequence of a Slovak SL-1 isolate of Tomato mosaic virus (ToMV) was determined from the next generation sequencing (NGS) data, further confirming a limited sequence divergence in this tobamovirus species. Tomato genotypes Monalbo, Mobaci and Moperou, respectively carrying the sus...
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| Format: | Article |
| Language: | English |
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Hanrimwon Publishing Company
2017-10-01
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| Series: | The Plant Pathology Journal |
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| Online Access: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5624493 |
| _version_ | 1819211219813269504 |
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| author | Nina Sihelská Zuzana Vozárová Lukáš Predajňa Katarína Šoltys Martina Hudcovicová Daniel Mihálik Ján Kraic Michaela Mrkvová Otakar Kúdela Miroslav Glasa |
| author_facet | Nina Sihelská Zuzana Vozárová Lukáš Predajňa Katarína Šoltys Martina Hudcovicová Daniel Mihálik Ján Kraic Michaela Mrkvová Otakar Kúdela Miroslav Glasa |
| author_sort | Nina Sihelská |
| collection | DOAJ |
| description | The complete genome sequence of a Slovak SL-1 isolate of Tomato mosaic virus (ToMV) was determined from the next generation sequencing (NGS) data, further confirming a limited sequence divergence in this tobamovirus species. Tomato genotypes Monalbo, Mobaci and Moperou, respectively carrying the susceptible tm-2 allele or the Tm-1 and Tm-2 resistant alleles, were tested for their susceptibility to ToMV SL-1. Although the three tomato genotypes accumulated ToMV SL-1 to similar amounts as judged by semi-quantitative DAS-ELISA, they showed variations in the rate of infection and symptomatology. Possible differences in the intra-isolate variability and polymorphism between viral populations propagating in these tomato genotypes were evaluated by analysis of the capsid protein (CP) encoding region. Irrespective of genotype infected, the intra-isolate haplotype structure showed the presence of the same highly dominant CP sequence and the low level of population diversity (0.08–0.19%). Our results suggest that ToMV CP encoding sequence is relatively stable in the viral population during its replication in vivo and provides further demonstration that RNA viruses may show high sequence stability, probably as a result of purifying selection. |
| first_indexed | 2024-12-23T06:23:36Z |
| format | Article |
| id | doaj.art-a3b5900f746f42fd8fc7672c3df1aa56 |
| institution | Directory Open Access Journal |
| issn | 1598-2254 |
| language | English |
| last_indexed | 2024-12-23T06:23:36Z |
| publishDate | 2017-10-01 |
| publisher | Hanrimwon Publishing Company |
| record_format | Article |
| series | The Plant Pathology Journal |
| spelling | doaj.art-a3b5900f746f42fd8fc7672c3df1aa562022-12-21T17:57:07ZengHanrimwon Publishing CompanyThe Plant Pathology Journal1598-22542017-10-0133550851310.5423/PPJ.NT.04.2017.0082PPJ.NT.04.2017.0082Experimental Infection of Different Tomato Genotypes with Tomato mosaic virus Led to a Low Viral Population Heterogeneity in the Capsid Protein Encoding RegionNina Sihelská0Zuzana Vozárová1Lukáš Predajňa2Katarína Šoltys3Martina Hudcovicová4Daniel Mihálik5Ján Kraic6Michaela Mrkvová7Otakar Kúdela8Miroslav Glasa9Institute of Virology, Biomedical Research Centre, Slovak Academy of Sciences, Dúbravská cesta 9, 84505 Bratislava, Slovak RepublicInstitute of Virology, Biomedical Research Centre, Slovak Academy of Sciences, Dúbravská cesta 9, 84505 Bratislava, Slovak RepublicInstitute of Virology, Biomedical Research Centre, Slovak Academy of Sciences, Dúbravská cesta 9, 84505 Bratislava, Slovak RepublicComenius University Science Park, Comenius University in Bratislava, Ilkovičova 8, 841 04, Bratislava, Slovak RepublicNational Agriculture and Food Centre-Research Institute of Plant Production, Bratislavská cesta 122, 921 68 Piešťany, Slovak RepublicNational Agriculture and Food Centre-Research Institute of Plant Production, Bratislavská cesta 122, 921 68 Piešťany, Slovak RepublicNational Agriculture and Food Centre-Research Institute of Plant Production, Bratislavská cesta 122, 921 68 Piešťany, Slovak RepublicDepartment of Biotechnologies, Faculty of Natural Sciences, University of SS. Cyril and Methodius, J. Herdu 2, 917 01 Trnava, Slovak RepublicZelseed Ltd., 930 36 Horná Potôň, Slovak RepublicInstitute of Virology, Biomedical Research Centre, Slovak Academy of Sciences, Dúbravská cesta 9, 84505 Bratislava, Slovak RepublicThe complete genome sequence of a Slovak SL-1 isolate of Tomato mosaic virus (ToMV) was determined from the next generation sequencing (NGS) data, further confirming a limited sequence divergence in this tobamovirus species. Tomato genotypes Monalbo, Mobaci and Moperou, respectively carrying the susceptible tm-2 allele or the Tm-1 and Tm-2 resistant alleles, were tested for their susceptibility to ToMV SL-1. Although the three tomato genotypes accumulated ToMV SL-1 to similar amounts as judged by semi-quantitative DAS-ELISA, they showed variations in the rate of infection and symptomatology. Possible differences in the intra-isolate variability and polymorphism between viral populations propagating in these tomato genotypes were evaluated by analysis of the capsid protein (CP) encoding region. Irrespective of genotype infected, the intra-isolate haplotype structure showed the presence of the same highly dominant CP sequence and the low level of population diversity (0.08–0.19%). Our results suggest that ToMV CP encoding sequence is relatively stable in the viral population during its replication in vivo and provides further demonstration that RNA viruses may show high sequence stability, probably as a result of purifying selection.https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5624493tobamovirusdiversityhaplotyperesistance gene |
| spellingShingle | Nina Sihelská Zuzana Vozárová Lukáš Predajňa Katarína Šoltys Martina Hudcovicová Daniel Mihálik Ján Kraic Michaela Mrkvová Otakar Kúdela Miroslav Glasa Experimental Infection of Different Tomato Genotypes with Tomato mosaic virus Led to a Low Viral Population Heterogeneity in the Capsid Protein Encoding Region The Plant Pathology Journal tobamovirus diversity haplotype resistance gene |
| title | Experimental Infection of Different Tomato Genotypes with Tomato mosaic virus Led to a Low Viral Population Heterogeneity in the Capsid Protein Encoding Region |
| title_full | Experimental Infection of Different Tomato Genotypes with Tomato mosaic virus Led to a Low Viral Population Heterogeneity in the Capsid Protein Encoding Region |
| title_fullStr | Experimental Infection of Different Tomato Genotypes with Tomato mosaic virus Led to a Low Viral Population Heterogeneity in the Capsid Protein Encoding Region |
| title_full_unstemmed | Experimental Infection of Different Tomato Genotypes with Tomato mosaic virus Led to a Low Viral Population Heterogeneity in the Capsid Protein Encoding Region |
| title_short | Experimental Infection of Different Tomato Genotypes with Tomato mosaic virus Led to a Low Viral Population Heterogeneity in the Capsid Protein Encoding Region |
| title_sort | experimental infection of different tomato genotypes with tomato mosaic virus led to a low viral population heterogeneity in the capsid protein encoding region |
| topic | tobamovirus diversity haplotype resistance gene |
| url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5624493 |
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