Chronic equine hepacivirus infection in an adult gelding with severe hepatopathy
Abstract Background: Equine hepacivirus (EqHV) in equids represents the closest homologue to hepatitis C virus (HCV) infecting humans. A majority of HCV infected patients develop a chronic course of infection leading to liver fibrosis, cirrhosis and liver failure. However, in horses mostly transient...
| Main Authors: | , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2019-08-01
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| Series: | Veterinary Medicine and Science |
| Subjects: | |
| Online Access: | https://doi.org/10.1002/vms3.181 |
| _version_ | 1818155346628182016 |
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| author | Birthe Tegtmeyer Julia Echelmeyer Vanessa M. Pfankuche Christina Puff Daniel Todt Nicole Fischer Andy Durham Karsten Feige Wolfgang Baumgärtner Eike Steinmann Jessika‐M. V. Cavalleri |
| author_facet | Birthe Tegtmeyer Julia Echelmeyer Vanessa M. Pfankuche Christina Puff Daniel Todt Nicole Fischer Andy Durham Karsten Feige Wolfgang Baumgärtner Eike Steinmann Jessika‐M. V. Cavalleri |
| author_sort | Birthe Tegtmeyer |
| collection | DOAJ |
| description | Abstract Background: Equine hepacivirus (EqHV) in equids represents the closest homologue to hepatitis C virus (HCV) infecting humans. A majority of HCV infected patients develop a chronic course of infection leading to liver fibrosis, cirrhosis and liver failure. However, in horses mostly transient mild subclinical infections are reported for EqHV to date. Objectives: EqHV can be involved in chronic liver diseases of horses. Methods: Biochemical parameters in serum samples were measured. Viral load was determined using qPCR. Next generation sequencing (NGS) of serum was performed. Liver tissue was stained with haematoxylin and eosin and analysed for viral RNA with fluorescent in situ‐hybridization. Results: The horse showed symptoms of severe hepatopathy and was chronically infected with EqHV. Viral RNA was detectable in the liver during disease. To rule out other infectious agents NGS was performed and showed the highest abundance for EqHV. The identified virus sequence was similar to other circulating equine hepaciviruses. Conclusions: EqHV can be associated with liver disease in horses. Whether it causes the disease or contributes in a multifactorial manner needs further investigation. |
| first_indexed | 2024-12-11T14:40:57Z |
| format | Article |
| id | doaj.art-a3b96c10591c43a6a160ad58d608c2f4 |
| institution | Directory Open Access Journal |
| issn | 2053-1095 |
| language | English |
| last_indexed | 2024-12-11T14:40:57Z |
| publishDate | 2019-08-01 |
| publisher | Wiley |
| record_format | Article |
| series | Veterinary Medicine and Science |
| spelling | doaj.art-a3b96c10591c43a6a160ad58d608c2f42022-12-22T01:01:56ZengWileyVeterinary Medicine and Science2053-10952019-08-015337237810.1002/vms3.181Chronic equine hepacivirus infection in an adult gelding with severe hepatopathyBirthe Tegtmeyer0Julia Echelmeyer1Vanessa M. Pfankuche2Christina Puff3Daniel Todt4Nicole Fischer5Andy Durham6Karsten Feige7Wolfgang Baumgärtner8Eike Steinmann9Jessika‐M. V. Cavalleri10Institute for Experimental Virology TWINCORE Centre for Experimental and Clinical Infection Research Medical School Hannover (MHH) Helmholtz Centre for Infection Research (HZI) Hannover GermanyClinic for Horses University of Veterinary Medicine Hannover, Foundation Hannover GermanyDepartment of Pathology University of Veterinary Medicine Hannover GermanyDepartment of Pathology University of Veterinary Medicine Hannover GermanyInstitute for Experimental Virology TWINCORE Centre for Experimental and Clinical Infection Research Medical School Hannover (MHH) Helmholtz Centre for Infection Research (HZI) Hannover GermanyInstitute of Medical Microbiology, Virology and Hygiene University Medical Centre Hamburg‐Eppendorf Hamburg GermanyLiphook Equine Hospital Liphook United KingdomClinic for Horses University of Veterinary Medicine Hannover, Foundation Hannover GermanyDepartment of Pathology University of Veterinary Medicine Hannover GermanyInstitute for Experimental Virology TWINCORE Centre for Experimental and Clinical Infection Research Medical School Hannover (MHH) Helmholtz Centre for Infection Research (HZI) Hannover GermanyClinic for Horses University of Veterinary Medicine Hannover, Foundation Hannover GermanyAbstract Background: Equine hepacivirus (EqHV) in equids represents the closest homologue to hepatitis C virus (HCV) infecting humans. A majority of HCV infected patients develop a chronic course of infection leading to liver fibrosis, cirrhosis and liver failure. However, in horses mostly transient mild subclinical infections are reported for EqHV to date. Objectives: EqHV can be involved in chronic liver diseases of horses. Methods: Biochemical parameters in serum samples were measured. Viral load was determined using qPCR. Next generation sequencing (NGS) of serum was performed. Liver tissue was stained with haematoxylin and eosin and analysed for viral RNA with fluorescent in situ‐hybridization. Results: The horse showed symptoms of severe hepatopathy and was chronically infected with EqHV. Viral RNA was detectable in the liver during disease. To rule out other infectious agents NGS was performed and showed the highest abundance for EqHV. The identified virus sequence was similar to other circulating equine hepaciviruses. Conclusions: EqHV can be associated with liver disease in horses. Whether it causes the disease or contributes in a multifactorial manner needs further investigation.https://doi.org/10.1002/vms3.181equine hepacivirushepatitis C virushepatopathyliverhepacivirus A |
| spellingShingle | Birthe Tegtmeyer Julia Echelmeyer Vanessa M. Pfankuche Christina Puff Daniel Todt Nicole Fischer Andy Durham Karsten Feige Wolfgang Baumgärtner Eike Steinmann Jessika‐M. V. Cavalleri Chronic equine hepacivirus infection in an adult gelding with severe hepatopathy Veterinary Medicine and Science equine hepacivirus hepatitis C virus hepatopathy liver hepacivirus A |
| title | Chronic equine hepacivirus infection in an adult gelding with severe hepatopathy |
| title_full | Chronic equine hepacivirus infection in an adult gelding with severe hepatopathy |
| title_fullStr | Chronic equine hepacivirus infection in an adult gelding with severe hepatopathy |
| title_full_unstemmed | Chronic equine hepacivirus infection in an adult gelding with severe hepatopathy |
| title_short | Chronic equine hepacivirus infection in an adult gelding with severe hepatopathy |
| title_sort | chronic equine hepacivirus infection in an adult gelding with severe hepatopathy |
| topic | equine hepacivirus hepatitis C virus hepatopathy liver hepacivirus A |
| url | https://doi.org/10.1002/vms3.181 |
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