Evx2-Hoxd13 intergenic region restricts enhancer association to Hoxd13 promoter.
Expression of Hox genes is tightly regulated in spatial and temporal domains. Evx2 is located next to Hoxd13 within 8 kb on the opposite DNA strand. Early in development, the pattern of Hoxd13 expression resembles that of Evx2 in limb and genital buds. After 10 dpc, however, Evx2 begins to be expres...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2007-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC1766471?pdf=render |
| _version_ | 1818506826094739456 |
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| author | Takumi Yamagishi Michiru Ozawa Chiho Ohtsuka Ritsuko Ohyama-Goto Takashi Kondo |
| author_facet | Takumi Yamagishi Michiru Ozawa Chiho Ohtsuka Ritsuko Ohyama-Goto Takashi Kondo |
| author_sort | Takumi Yamagishi |
| collection | DOAJ |
| description | Expression of Hox genes is tightly regulated in spatial and temporal domains. Evx2 is located next to Hoxd13 within 8 kb on the opposite DNA strand. Early in development, the pattern of Hoxd13 expression resembles that of Evx2 in limb and genital buds. After 10 dpc, however, Evx2 begins to be expressed in CNS as well. We analyzed the region responsible for these differences using ES cell techniques, and found that the intergenic region between Evx2 and Hoxd13 behaves as a boundary element that functions differentially in space and time, specifically in the development of limbs, genital bud, and brain. This boundary element comprises a large sequence spanning several kilobases that can be divided into at least two units: a constitutive boundary element, which blocks transcription regulatory influences from the chromosomal environment, and a regulatory element, which controls the function of the constitutive boundary element in time and space. |
| first_indexed | 2024-12-10T22:09:52Z |
| format | Article |
| id | doaj.art-a3b9b364090b4e60a62462c6b08362fd |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-10T22:09:52Z |
| publishDate | 2007-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-a3b9b364090b4e60a62462c6b08362fd2022-12-22T01:31:38ZengPublic Library of Science (PLoS)PLoS ONE1932-62032007-01-0121e17510.1371/journal.pone.0000175Evx2-Hoxd13 intergenic region restricts enhancer association to Hoxd13 promoter.Takumi YamagishiMichiru OzawaChiho OhtsukaRitsuko Ohyama-GotoTakashi KondoExpression of Hox genes is tightly regulated in spatial and temporal domains. Evx2 is located next to Hoxd13 within 8 kb on the opposite DNA strand. Early in development, the pattern of Hoxd13 expression resembles that of Evx2 in limb and genital buds. After 10 dpc, however, Evx2 begins to be expressed in CNS as well. We analyzed the region responsible for these differences using ES cell techniques, and found that the intergenic region between Evx2 and Hoxd13 behaves as a boundary element that functions differentially in space and time, specifically in the development of limbs, genital bud, and brain. This boundary element comprises a large sequence spanning several kilobases that can be divided into at least two units: a constitutive boundary element, which blocks transcription regulatory influences from the chromosomal environment, and a regulatory element, which controls the function of the constitutive boundary element in time and space.http://europepmc.org/articles/PMC1766471?pdf=render |
| spellingShingle | Takumi Yamagishi Michiru Ozawa Chiho Ohtsuka Ritsuko Ohyama-Goto Takashi Kondo Evx2-Hoxd13 intergenic region restricts enhancer association to Hoxd13 promoter. PLoS ONE |
| title | Evx2-Hoxd13 intergenic region restricts enhancer association to Hoxd13 promoter. |
| title_full | Evx2-Hoxd13 intergenic region restricts enhancer association to Hoxd13 promoter. |
| title_fullStr | Evx2-Hoxd13 intergenic region restricts enhancer association to Hoxd13 promoter. |
| title_full_unstemmed | Evx2-Hoxd13 intergenic region restricts enhancer association to Hoxd13 promoter. |
| title_short | Evx2-Hoxd13 intergenic region restricts enhancer association to Hoxd13 promoter. |
| title_sort | evx2 hoxd13 intergenic region restricts enhancer association to hoxd13 promoter |
| url | http://europepmc.org/articles/PMC1766471?pdf=render |
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