Detection and Morphological Analysis of Micro-Ruptured Cortical Arteries in Subdural Hematoma: Three-Dimensional Visualization Using the Tissue-Clearing Clear, Unobstructed, Brain/Body Imaging Cocktails and Computational Analysis Method
One of the causes of bleeding in subdural hematoma is cortical artery rupture, which is difficult to detect at autopsy. Therefore, reports of autopsy cases with this condition are limited and hence, the pathogenesis of subdural hematoma remains unclear. Herein, for the detection and morphological an...
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MDPI AG
2022-11-01
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| Series: | Diagnostics |
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| Online Access: | https://www.mdpi.com/2075-4418/12/11/2875 |
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| author | Kazuhisa Funayama Kazuki Tainaka Akihide Koyama Rieka Katsuragi-Go Natsumi Nishikawa-Harada Ryoko Higuchi Takashi Aoyama Hiraku Watanabe Naoya Takahashi Hisakazu Takatsuka |
| author_facet | Kazuhisa Funayama Kazuki Tainaka Akihide Koyama Rieka Katsuragi-Go Natsumi Nishikawa-Harada Ryoko Higuchi Takashi Aoyama Hiraku Watanabe Naoya Takahashi Hisakazu Takatsuka |
| author_sort | Kazuhisa Funayama |
| collection | DOAJ |
| description | One of the causes of bleeding in subdural hematoma is cortical artery rupture, which is difficult to detect at autopsy. Therefore, reports of autopsy cases with this condition are limited and hence, the pathogenesis of subdural hematoma remains unclear. Herein, for the detection and morphological analysis of cortical artery ruptures as the bleeding sources of subdural hematoma, we used the tissue-clearing CUBIC (clear, unobstructed, brain/body imaging cocktails and computational analysis) method with light-sheet fluorescence microscopy and reconstructed the two-dimensional and three-dimensional images. Using the CUBIC method, we could clearly visualize and detect cortical artery ruptures that were missed by conventional methods. Indeed, the CUBIC method enables three-dimensional morphological analysis of cortical arteries including the ruptured area, and the creation of cross-sectional two-dimensional images in any direction, which are similar to histopathological images. This highlights the effectiveness of the CUBIC method for subdural hematoma analysis. |
| first_indexed | 2024-03-09T18:23:39Z |
| format | Article |
| id | doaj.art-a3b9d168e1ea45798eec6a417c990154 |
| institution | Directory Open Access Journal |
| issn | 2075-4418 |
| language | English |
| last_indexed | 2024-03-09T18:23:39Z |
| publishDate | 2022-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Diagnostics |
| spelling | doaj.art-a3b9d168e1ea45798eec6a417c9901542023-11-24T08:05:41ZengMDPI AGDiagnostics2075-44182022-11-011211287510.3390/diagnostics12112875Detection and Morphological Analysis of Micro-Ruptured Cortical Arteries in Subdural Hematoma: Three-Dimensional Visualization Using the Tissue-Clearing Clear, Unobstructed, Brain/Body Imaging Cocktails and Computational Analysis MethodKazuhisa Funayama0Kazuki Tainaka1Akihide Koyama2Rieka Katsuragi-Go3Natsumi Nishikawa-Harada4Ryoko Higuchi5Takashi Aoyama6Hiraku Watanabe7Naoya Takahashi8Hisakazu Takatsuka9Division of Legal Medicine, Department of Community Preventive Medicine, Graduate School of Medicine and Dental Sciences, Niigata University, Niigata 951-8510, JapanDepartment of System Pathology for Neurological Disorders, Basic Neuroscience Branch, Brain Research Institute, Niigata University, Niigata 951-8510, JapanDivision of Legal Medicine, Department of Community Preventive Medicine, Graduate School of Medicine and Dental Sciences, Niigata University, Niigata 951-8510, JapanDivision of Legal Medicine, Department of Community Preventive Medicine, Graduate School of Medicine and Dental Sciences, Niigata University, Niigata 951-8510, JapanDivision of Legal Medicine, Department of Community Preventive Medicine, Graduate School of Medicine and Dental Sciences, Niigata University, Niigata 951-8510, JapanDivision of Legal Medicine, Department of Community Preventive Medicine, Graduate School of Medicine and Dental Sciences, Niigata University, Niigata 951-8510, JapanDivision of Legal Medicine, Department of Community Preventive Medicine, Graduate School of Medicine and Dental Sciences, Niigata University, Niigata 951-8510, JapanDivision of Legal Medicine, Department of Community Preventive Medicine, Graduate School of Medicine and Dental Sciences, Niigata University, Niigata 951-8510, JapanCenter of Cause of Death Investigation, Graduate School of Medical and Dental Sciences, Niigata University, Niigata 951-8510, JapanDivision of Legal Medicine, Department of Community Preventive Medicine, Graduate School of Medicine and Dental Sciences, Niigata University, Niigata 951-8510, JapanOne of the causes of bleeding in subdural hematoma is cortical artery rupture, which is difficult to detect at autopsy. Therefore, reports of autopsy cases with this condition are limited and hence, the pathogenesis of subdural hematoma remains unclear. Herein, for the detection and morphological analysis of cortical artery ruptures as the bleeding sources of subdural hematoma, we used the tissue-clearing CUBIC (clear, unobstructed, brain/body imaging cocktails and computational analysis) method with light-sheet fluorescence microscopy and reconstructed the two-dimensional and three-dimensional images. Using the CUBIC method, we could clearly visualize and detect cortical artery ruptures that were missed by conventional methods. Indeed, the CUBIC method enables three-dimensional morphological analysis of cortical arteries including the ruptured area, and the creation of cross-sectional two-dimensional images in any direction, which are similar to histopathological images. This highlights the effectiveness of the CUBIC method for subdural hematoma analysis.https://www.mdpi.com/2075-4418/12/11/2875subdural hematomacortical arteryarterial rupture3D imagingCUBIC |
| spellingShingle | Kazuhisa Funayama Kazuki Tainaka Akihide Koyama Rieka Katsuragi-Go Natsumi Nishikawa-Harada Ryoko Higuchi Takashi Aoyama Hiraku Watanabe Naoya Takahashi Hisakazu Takatsuka Detection and Morphological Analysis of Micro-Ruptured Cortical Arteries in Subdural Hematoma: Three-Dimensional Visualization Using the Tissue-Clearing Clear, Unobstructed, Brain/Body Imaging Cocktails and Computational Analysis Method Diagnostics subdural hematoma cortical artery arterial rupture 3D imaging CUBIC |
| title | Detection and Morphological Analysis of Micro-Ruptured Cortical Arteries in Subdural Hematoma: Three-Dimensional Visualization Using the Tissue-Clearing Clear, Unobstructed, Brain/Body Imaging Cocktails and Computational Analysis Method |
| title_full | Detection and Morphological Analysis of Micro-Ruptured Cortical Arteries in Subdural Hematoma: Three-Dimensional Visualization Using the Tissue-Clearing Clear, Unobstructed, Brain/Body Imaging Cocktails and Computational Analysis Method |
| title_fullStr | Detection and Morphological Analysis of Micro-Ruptured Cortical Arteries in Subdural Hematoma: Three-Dimensional Visualization Using the Tissue-Clearing Clear, Unobstructed, Brain/Body Imaging Cocktails and Computational Analysis Method |
| title_full_unstemmed | Detection and Morphological Analysis of Micro-Ruptured Cortical Arteries in Subdural Hematoma: Three-Dimensional Visualization Using the Tissue-Clearing Clear, Unobstructed, Brain/Body Imaging Cocktails and Computational Analysis Method |
| title_short | Detection and Morphological Analysis of Micro-Ruptured Cortical Arteries in Subdural Hematoma: Three-Dimensional Visualization Using the Tissue-Clearing Clear, Unobstructed, Brain/Body Imaging Cocktails and Computational Analysis Method |
| title_sort | detection and morphological analysis of micro ruptured cortical arteries in subdural hematoma three dimensional visualization using the tissue clearing clear unobstructed brain body imaging cocktails and computational analysis method |
| topic | subdural hematoma cortical artery arterial rupture 3D imaging CUBIC |
| url | https://www.mdpi.com/2075-4418/12/11/2875 |
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