Antioxidant and cytotoxic activity of Tecoma stans against lung cancer cell line (A549)
ABSTRACT Human have been constantly using plants and plant products to overcome many diseases. The antioxidant property of the plant sources is studied to obtain an efficacious drug against cancer. The objectives of the present study is to evaluate the antioxidant and cytotoxic activity of the Tecom...
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Universidade de São Paulo
2017-07-01
|
| Series: | Brazilian Journal of Pharmaceutical Sciences |
| Subjects: | |
| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502017000300605&lng=en&tlng=en |
| _version_ | 1819022826613506048 |
|---|---|
| author | Jayachandran Philip Robinson Kumaresan Suriya Ramasamy Subbaiya Ponnusamy Ponmurugan |
| author_facet | Jayachandran Philip Robinson Kumaresan Suriya Ramasamy Subbaiya Ponnusamy Ponmurugan |
| author_sort | Jayachandran Philip Robinson |
| collection | DOAJ |
| description | ABSTRACT Human have been constantly using plants and plant products to overcome many diseases. The antioxidant property of the plant sources is studied to obtain an efficacious drug against cancer. The objectives of the present study is to evaluate the antioxidant and cytotoxic activity of the Tecoma stans extracts against lung cancer cell line in comparison with vincristine drug. The antioxidant activity was studied using the standard DPPH assay and the cytotoxic activity using MTT assay. DPPH assay results show that methanolic extract of T. stans in higher concentration show better antioxidant potential than the standard L-ascorbic acid. They exhibited strong antioxidant potential at 20 µg/mL concentration. The absorbance at 517 nm showed that in the range of 0.201-0.0203 compared to that of absorbance of ascorbic acid at 0.023.Cytotoxic activity was studied using MTT assay which showed that the increase in concentration of extract increases the cell death. At 100µg/mL concentration there is an increased cytotoxic activity, i.e., 99% of cell inhibition. The results of antioxidant and anticancerous activity may be positively correlated. |
| first_indexed | 2024-12-21T04:29:10Z |
| format | Article |
| id | doaj.art-a3ba8c39503b44c9aa9146638a6b6731 |
| institution | Directory Open Access Journal |
| issn | 2175-9790 |
| language | English |
| last_indexed | 2024-12-21T04:29:10Z |
| publishDate | 2017-07-01 |
| publisher | Universidade de São Paulo |
| record_format | Article |
| series | Brazilian Journal of Pharmaceutical Sciences |
| spelling | doaj.art-a3ba8c39503b44c9aa9146638a6b67312022-12-21T19:16:00ZengUniversidade de São PauloBrazilian Journal of Pharmaceutical Sciences2175-97902017-07-0153310.1590/s2175-97902017000300204S1984-82502017000300605Antioxidant and cytotoxic activity of Tecoma stans against lung cancer cell line (A549)Jayachandran Philip RobinsonKumaresan SuriyaRamasamy SubbaiyaPonnusamy PonmuruganABSTRACT Human have been constantly using plants and plant products to overcome many diseases. The antioxidant property of the plant sources is studied to obtain an efficacious drug against cancer. The objectives of the present study is to evaluate the antioxidant and cytotoxic activity of the Tecoma stans extracts against lung cancer cell line in comparison with vincristine drug. The antioxidant activity was studied using the standard DPPH assay and the cytotoxic activity using MTT assay. DPPH assay results show that methanolic extract of T. stans in higher concentration show better antioxidant potential than the standard L-ascorbic acid. They exhibited strong antioxidant potential at 20 µg/mL concentration. The absorbance at 517 nm showed that in the range of 0.201-0.0203 compared to that of absorbance of ascorbic acid at 0.023.Cytotoxic activity was studied using MTT assay which showed that the increase in concentration of extract increases the cell death. At 100µg/mL concentration there is an increased cytotoxic activity, i.e., 99% of cell inhibition. The results of antioxidant and anticancerous activity may be positively correlated.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502017000300605&lng=en&tlng=enTecoma stans/extract/cytotoxicityTecoma stans/extract/antioxidant activityFree radical scavengingPlant extractsLung cancer cell line/study/drugs |
| spellingShingle | Jayachandran Philip Robinson Kumaresan Suriya Ramasamy Subbaiya Ponnusamy Ponmurugan Antioxidant and cytotoxic activity of Tecoma stans against lung cancer cell line (A549) Brazilian Journal of Pharmaceutical Sciences Tecoma stans/extract/cytotoxicity Tecoma stans/extract/antioxidant activity Free radical scavenging Plant extracts Lung cancer cell line/study/drugs |
| title | Antioxidant and cytotoxic activity of Tecoma stans against lung cancer cell line (A549) |
| title_full | Antioxidant and cytotoxic activity of Tecoma stans against lung cancer cell line (A549) |
| title_fullStr | Antioxidant and cytotoxic activity of Tecoma stans against lung cancer cell line (A549) |
| title_full_unstemmed | Antioxidant and cytotoxic activity of Tecoma stans against lung cancer cell line (A549) |
| title_short | Antioxidant and cytotoxic activity of Tecoma stans against lung cancer cell line (A549) |
| title_sort | antioxidant and cytotoxic activity of tecoma stans against lung cancer cell line a549 |
| topic | Tecoma stans/extract/cytotoxicity Tecoma stans/extract/antioxidant activity Free radical scavenging Plant extracts Lung cancer cell line/study/drugs |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502017000300605&lng=en&tlng=en |
| work_keys_str_mv | AT jayachandranphiliprobinson antioxidantandcytotoxicactivityoftecomastansagainstlungcancercelllinea549 AT kumaresansuriya antioxidantandcytotoxicactivityoftecomastansagainstlungcancercelllinea549 AT ramasamysubbaiya antioxidantandcytotoxicactivityoftecomastansagainstlungcancercelllinea549 AT ponnusamyponmurugan antioxidantandcytotoxicactivityoftecomastansagainstlungcancercelllinea549 |