Establishment of SIAISi021-A, an induced pluripotent stem cell (iPSC) line from a 71-year-old Chinese Han male with Alzheimer’s Disease (AD) having two copies of APOE4/4 allele
A 71-year-old Han male from China contributed peripheral blood mononuclear cells (PBMCs). Non-integrative Sendai viral vectors containing reprogramming factors OCT4, KLF4, SOX2 and C-MYC were used to reprogram PBMCs. Pluripotency makers confirmed the pluripotency of transgene-free induced pluripoten...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2022-08-01
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| Series: | Stem Cell Research |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S187350612200215X |
| _version_ | 1828518113410285568 |
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| author | Yanfei Ding Haijuan Chen Jian Zhao Ying Wang Yulei Deng |
| author_facet | Yanfei Ding Haijuan Chen Jian Zhao Ying Wang Yulei Deng |
| author_sort | Yanfei Ding |
| collection | DOAJ |
| description | A 71-year-old Han male from China contributed peripheral blood mononuclear cells (PBMCs). Non-integrative Sendai viral vectors containing reprogramming factors OCT4, KLF4, SOX2 and C-MYC were used to reprogram PBMCs. Pluripotency makers confirmed the pluripotency of transgene-free induced pluripotent stem cell (iPSC). The ability of iPSC to spontaneously differentiate three germ layers in vitro confirmed the pluripotency of iPSC. The iPSC line displayed a normal karyotype. The newly generated human iPSC SIAISi021-A can be used for studying further disease mechanisms of Alzheimer’s Disease (AD). |
| first_indexed | 2024-12-11T18:50:44Z |
| format | Article |
| id | doaj.art-a3bb6e12c31e43749a80fffbd4d9d634 |
| institution | Directory Open Access Journal |
| issn | 1873-5061 |
| language | English |
| last_indexed | 2024-12-11T18:50:44Z |
| publishDate | 2022-08-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Stem Cell Research |
| spelling | doaj.art-a3bb6e12c31e43749a80fffbd4d9d6342022-12-22T00:54:18ZengElsevierStem Cell Research1873-50612022-08-0163102866Establishment of SIAISi021-A, an induced pluripotent stem cell (iPSC) line from a 71-year-old Chinese Han male with Alzheimer’s Disease (AD) having two copies of APOE4/4 alleleYanfei Ding0Haijuan Chen1Jian Zhao2Ying Wang3Yulei Deng4Department of Neurology & Institute of Neurology, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China; Department of Neurology, Ruijin Hospital Luwan Branch, Shanghai Jiao Tong University School of Medicine, Shanghai, ChinaDepartment of Neurology & Institute of Neurology, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China; Department of Neurology, Ruijin Hospital Luwan Branch, Shanghai Jiao Tong University School of Medicine, Shanghai, ChinaLab of Regenerative Medicine and Neural Repair, Shanghai Institute for Advanced Immunochemical Studies, and School of Life Science and Technology, ShanghaiTech, ChinaLab of Regenerative Medicine and Neural Repair, Shanghai Institute for Advanced Immunochemical Studies, and School of Life Science and Technology, ShanghaiTech, China; Corresponding authors at: Department of Neurology & Institute of Neurology, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China (Y. Deng); Lab of Regenerative Medicine and Neural Repair, Shanghai Institute for Advanced Immunochemical Studies, and School of Life Science and technology, ShanghaiTech (Y. Wang).Department of Neurology & Institute of Neurology, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China; Department of Neurology, Ruijin Hospital Luwan Branch, Shanghai Jiao Tong University School of Medicine, Shanghai, China; Corresponding authors at: Department of Neurology & Institute of Neurology, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China (Y. Deng); Lab of Regenerative Medicine and Neural Repair, Shanghai Institute for Advanced Immunochemical Studies, and School of Life Science and technology, ShanghaiTech (Y. Wang).A 71-year-old Han male from China contributed peripheral blood mononuclear cells (PBMCs). Non-integrative Sendai viral vectors containing reprogramming factors OCT4, KLF4, SOX2 and C-MYC were used to reprogram PBMCs. Pluripotency makers confirmed the pluripotency of transgene-free induced pluripotent stem cell (iPSC). The ability of iPSC to spontaneously differentiate three germ layers in vitro confirmed the pluripotency of iPSC. The iPSC line displayed a normal karyotype. The newly generated human iPSC SIAISi021-A can be used for studying further disease mechanisms of Alzheimer’s Disease (AD).http://www.sciencedirect.com/science/article/pii/S187350612200215X |
| spellingShingle | Yanfei Ding Haijuan Chen Jian Zhao Ying Wang Yulei Deng Establishment of SIAISi021-A, an induced pluripotent stem cell (iPSC) line from a 71-year-old Chinese Han male with Alzheimer’s Disease (AD) having two copies of APOE4/4 allele Stem Cell Research |
| title | Establishment of SIAISi021-A, an induced pluripotent stem cell (iPSC) line from a 71-year-old Chinese Han male with Alzheimer’s Disease (AD) having two copies of APOE4/4 allele |
| title_full | Establishment of SIAISi021-A, an induced pluripotent stem cell (iPSC) line from a 71-year-old Chinese Han male with Alzheimer’s Disease (AD) having two copies of APOE4/4 allele |
| title_fullStr | Establishment of SIAISi021-A, an induced pluripotent stem cell (iPSC) line from a 71-year-old Chinese Han male with Alzheimer’s Disease (AD) having two copies of APOE4/4 allele |
| title_full_unstemmed | Establishment of SIAISi021-A, an induced pluripotent stem cell (iPSC) line from a 71-year-old Chinese Han male with Alzheimer’s Disease (AD) having two copies of APOE4/4 allele |
| title_short | Establishment of SIAISi021-A, an induced pluripotent stem cell (iPSC) line from a 71-year-old Chinese Han male with Alzheimer’s Disease (AD) having two copies of APOE4/4 allele |
| title_sort | establishment of siaisi021 a an induced pluripotent stem cell ipsc line from a 71 year old chinese han male with alzheimer s disease ad having two copies of apoe4 4 allele |
| url | http://www.sciencedirect.com/science/article/pii/S187350612200215X |
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