Microbiological and molecular characterization of Vibrio cholera and Vibrio parahaemolyticus isolated from Tilapia fish (Oreochromis niloticus
Vibrio species are zoonotic pathogens that can affect humans by different routes such as ingestion or contact causing gastrointestinal diseases and wound infection. This study aimed to detect V. cholera and V. parahaemolyticus in Nile Tilapia fish collected from Aswan Governorate, Egypt. A total of...
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| Format: | Article |
| Language: | English |
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South Valley University
2023-07-01
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| Series: | SVU-International Journal of Veterinary Sciences |
| Subjects: | |
| Online Access: | https://svu.journals.ekb.eg/article_307069.html |
| _version_ | 1797733492640972800 |
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| author | Ekram Abd elaziz Ali Mohamed W. Abd Al-azeem Waleed Younis |
| author_facet | Ekram Abd elaziz Ali Mohamed W. Abd Al-azeem Waleed Younis |
| author_sort | Ekram Abd elaziz Ali |
| collection | DOAJ |
| description | Vibrio species are zoonotic pathogens that can affect humans by different routes such as ingestion or contact causing gastrointestinal diseases and wound infection. This study aimed to detect V. cholera and V. parahaemolyticus in Nile Tilapia fish collected from Aswan Governorate, Egypt. A total of 52 muscle samples were collected from Tilapia fish and were subjected to microbiological and molecular characterization. Alkaline peptone water media was used for the enrichment of the samples then followed by inoculation onto thiosulfate citrate bile salt sucrose (TCBS) agar media for the isolation of Vibrio species, biochemical tests were performed to identify V. cholera and V. parahaemolyticus, and then they were confirmed by Vibrio genus-specific gene and virulence genes by PCR . Out of 52 fish muscle samples (Nile Tilapia) 32 appeared as yellow colonies, 2 samples showed green colonies and 18 samples showed mixed yellow and green on TCBS agar. Only 40 samples show biochemically positive for Vibrio species. 15 random samples were amplified to 16srRNA gene by PCR technique for more accurate identification resulting in 14 isolates being positive to 16srRNA, 8 isolates positive for sodB gene-specific of V. cholera, and 6 isolates positive for toxR gene-specific of V. parahaemolyticus. Out of 8 V. cholera isolates were found high resistance rate to amoxiclav (87.5%), gentamicin (75%), chloramphenicol (50 %) and ampicillin (50 %). Conversely, (50 %) sensitive to tetracycline, ciprofloxacin and, trimethoprimsulphamethoxazole, and the average MRA of V. cholera was 0.48. On the other hand, all 6 V. parahaemolyticus isolates were resistant to amoxiclav (100%), tetracycline (100%), gentamicin (100%) and chloramphenicol (100%) However, all isolates sensitive to trimethoprimsulphamethazole (100%) but half of them sensitive to ciprofloxacin (50%) and ampicillin (50%), and the average MRA of V. parahaemolyticus was 0.59. In conclusion, this study showed that V. cholera is the most dominant pathogenic one then followed by V. parahaemolyticus in Nile tilapia fish resulting in economic losses and causing public health problems. |
| first_indexed | 2024-03-12T12:28:54Z |
| format | Article |
| id | doaj.art-a3bb9a915c554863af5825371f47c3dd |
| institution | Directory Open Access Journal |
| issn | 2535-1826 2535-1877 |
| language | English |
| last_indexed | 2024-03-12T12:28:54Z |
| publishDate | 2023-07-01 |
| publisher | South Valley University |
| record_format | Article |
| series | SVU-International Journal of Veterinary Sciences |
| spelling | doaj.art-a3bb9a915c554863af5825371f47c3dd2023-08-29T17:26:31ZengSouth Valley UniversitySVU-International Journal of Veterinary Sciences2535-18262535-18772023-07-016311610.21608/SVU.2023.203762.1266Microbiological and molecular characterization of Vibrio cholera and Vibrio parahaemolyticus isolated from Tilapia fish (Oreochromis niloticusEkram Abd elaziz Ali0 Mohamed W. Abd Al-azeem1 Waleed Younis2microbiology department , faculty of veterinary medicine, south valley universitymicrobiology department , faculty of veterinary medicine, south valley universitymicrobiology department , faculty of veterinary medicine, south valley universityVibrio species are zoonotic pathogens that can affect humans by different routes such as ingestion or contact causing gastrointestinal diseases and wound infection. This study aimed to detect V. cholera and V. parahaemolyticus in Nile Tilapia fish collected from Aswan Governorate, Egypt. A total of 52 muscle samples were collected from Tilapia fish and were subjected to microbiological and molecular characterization. Alkaline peptone water media was used for the enrichment of the samples then followed by inoculation onto thiosulfate citrate bile salt sucrose (TCBS) agar media for the isolation of Vibrio species, biochemical tests were performed to identify V. cholera and V. parahaemolyticus, and then they were confirmed by Vibrio genus-specific gene and virulence genes by PCR . Out of 52 fish muscle samples (Nile Tilapia) 32 appeared as yellow colonies, 2 samples showed green colonies and 18 samples showed mixed yellow and green on TCBS agar. Only 40 samples show biochemically positive for Vibrio species. 15 random samples were amplified to 16srRNA gene by PCR technique for more accurate identification resulting in 14 isolates being positive to 16srRNA, 8 isolates positive for sodB gene-specific of V. cholera, and 6 isolates positive for toxR gene-specific of V. parahaemolyticus. Out of 8 V. cholera isolates were found high resistance rate to amoxiclav (87.5%), gentamicin (75%), chloramphenicol (50 %) and ampicillin (50 %). Conversely, (50 %) sensitive to tetracycline, ciprofloxacin and, trimethoprimsulphamethoxazole, and the average MRA of V. cholera was 0.48. On the other hand, all 6 V. parahaemolyticus isolates were resistant to amoxiclav (100%), tetracycline (100%), gentamicin (100%) and chloramphenicol (100%) However, all isolates sensitive to trimethoprimsulphamethazole (100%) but half of them sensitive to ciprofloxacin (50%) and ampicillin (50%), and the average MRA of V. parahaemolyticus was 0.59. In conclusion, this study showed that V. cholera is the most dominant pathogenic one then followed by V. parahaemolyticus in Nile tilapia fish resulting in economic losses and causing public health problems. https://svu.journals.ekb.eg/article_307069.htmlvibriotilapia fishpcrresistant |
| spellingShingle | Ekram Abd elaziz Ali Mohamed W. Abd Al-azeem Waleed Younis Microbiological and molecular characterization of Vibrio cholera and Vibrio parahaemolyticus isolated from Tilapia fish (Oreochromis niloticus SVU-International Journal of Veterinary Sciences vibrio tilapia fish pcr resistant |
| title | Microbiological and molecular characterization of Vibrio cholera and Vibrio parahaemolyticus isolated from Tilapia fish (Oreochromis niloticus |
| title_full | Microbiological and molecular characterization of Vibrio cholera and Vibrio parahaemolyticus isolated from Tilapia fish (Oreochromis niloticus |
| title_fullStr | Microbiological and molecular characterization of Vibrio cholera and Vibrio parahaemolyticus isolated from Tilapia fish (Oreochromis niloticus |
| title_full_unstemmed | Microbiological and molecular characterization of Vibrio cholera and Vibrio parahaemolyticus isolated from Tilapia fish (Oreochromis niloticus |
| title_short | Microbiological and molecular characterization of Vibrio cholera and Vibrio parahaemolyticus isolated from Tilapia fish (Oreochromis niloticus |
| title_sort | microbiological and molecular characterization of vibrio cholera and vibrio parahaemolyticus isolated from tilapia fish oreochromis niloticus |
| topic | vibrio tilapia fish pcr resistant |
| url | https://svu.journals.ekb.eg/article_307069.html |
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