A Pilot Study of Whole-Blood Transcriptomic Analysis to Identify Genes Associated with Repetitive Low-Level Blast Exposure in Career Breachers
Repetitive low-level blast exposure is one of the major occupational health concerns among US military service members and law enforcement. This study seeks to identify gene expression using microRNA and RNA sequencing in whole-blood samples from experienced breachers and unexposed controls. We perf...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-03-01
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| Series: | Biomedicines |
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| Online Access: | https://www.mdpi.com/2227-9059/10/3/690 |
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| author | Rany Vorn Katie A. Edwards James Hentig Sijung Yun Hyung-Suk Kim Chen Lai Christina Devoto Angela M. Yarnell Elena Polejaeva Kristine C. Dell Matthew L. LoPresti Peter Walker Walter Carr James R. Stone Stephen T. Ahlers Jessica M. Gill |
| author_facet | Rany Vorn Katie A. Edwards James Hentig Sijung Yun Hyung-Suk Kim Chen Lai Christina Devoto Angela M. Yarnell Elena Polejaeva Kristine C. Dell Matthew L. LoPresti Peter Walker Walter Carr James R. Stone Stephen T. Ahlers Jessica M. Gill |
| author_sort | Rany Vorn |
| collection | DOAJ |
| description | Repetitive low-level blast exposure is one of the major occupational health concerns among US military service members and law enforcement. This study seeks to identify gene expression using microRNA and RNA sequencing in whole-blood samples from experienced breachers and unexposed controls. We performed experimental RNA sequencing using Illumina’s HiSeq 2500 Sequencing System, and microRNA analysis using NanoString Technology nCounter miRNA expression panel in whole-blood total RNA samples from 15 experienced breachers and 14 age-, sex-, and race-matched unexposed controls. We identified 10 significantly dysregulated genes between experienced breachers and unexposed controls, with FDR corrected <0.05: One upregulated gene, <i>LINC00996</i> (<i>long intergenic non-protein coding RNA 996</i>); and nine downregulated genes, <i>IGLV3-16</i> (<i>immunoglobulin lambda variable 3-16</i>), <i>CD200</i> (<i>CD200 molecule</i>), <i>LILRB5</i> (<i>leukocyte immunoglobulin-like receptor B5</i>), <i>ZNF667-AS1</i> (<i>ZNF667 antisense RNA 1</i>), <i>LMOD1</i> (<i>leiomodin 1</i>), <i>CNTNAP2</i> (<i>contactin-associated protein 2</i>), <i>EVPL</i> (<i>envoplakin</i>), <i>DPF3</i> (<i>double PHD fingers 3</i>), and <i>IGHV4-34</i> (<i>immunoglobulin heavy variable 4-34</i>). The dysregulated gene expressions reported here have been associated with chronic inflammation and immune response, suggesting that these pathways may relate to the risk of lasting neurological symptoms following high exposures to blast over a career. |
| first_indexed | 2024-03-09T20:05:02Z |
| format | Article |
| id | doaj.art-a3bbf257171841298c727a9e19dc1d3e |
| institution | Directory Open Access Journal |
| issn | 2227-9059 |
| language | English |
| last_indexed | 2024-03-09T20:05:02Z |
| publishDate | 2022-03-01 |
| publisher | MDPI AG |
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| series | Biomedicines |
| spelling | doaj.art-a3bbf257171841298c727a9e19dc1d3e2023-11-24T00:33:43ZengMDPI AGBiomedicines2227-90592022-03-0110369010.3390/biomedicines10030690A Pilot Study of Whole-Blood Transcriptomic Analysis to Identify Genes Associated with Repetitive Low-Level Blast Exposure in Career BreachersRany Vorn0Katie A. Edwards1James Hentig2Sijung Yun3Hyung-Suk Kim4Chen Lai5Christina Devoto6Angela M. Yarnell7Elena Polejaeva8Kristine C. Dell9Matthew L. LoPresti10Peter Walker11Walter Carr12James R. Stone13Stephen T. Ahlers14Jessica M. Gill15School of Nursing and Medicine, Johns Hopkins University, Baltimore, MD 21205, USANational Institute of Nursing Research, National Institutes of Health, Bethesda, MD 20892, USAWarrior Recovery Center, Evans Army Community Hospital, Fort Carson, CO 80913, USAPredictiv Care, Inc., Mountain View, CA 94086, USANational Institute of Nursing Research, National Institutes of Health, Bethesda, MD 20892, USACenter for Neuroscience and Regenerative Medicine, Uniformed Services University of the Health Sciences, Bethesda, MD 20814, USANational Institute of Nursing Research, National Institutes of Health, Bethesda, MD 20892, USAMilitary Emergency Medicine Department, Uniformed Services University of the Health Sciences, Bethesda, MD 20814, USAVA San Diego Healthcare System, San Diego, CA 92161, USADepartment of Psychology, Pennsylvania State University, State College, PA 16802, USACenter for Military Psychiatry and Neuroscience, Walter Reed Army Institute of Research, Silver Spring, MD 20910, USAProgram Executive Office Unmanned and Small Combatants, NAVSEA, Washington, DC 20003, USACenter for Military Psychiatry and Neuroscience, Walter Reed Army Institute of Research, Silver Spring, MD 20910, USADepartment of Radiology and Medical Imaging, University of Virginia, Charlottesville, VA 22903, USAOperational and Undersea Medicine Directorate, Naval Medical Research Center, Silver Spring, MD 20910, USASchool of Nursing and Medicine, Johns Hopkins University, Baltimore, MD 21205, USARepetitive low-level blast exposure is one of the major occupational health concerns among US military service members and law enforcement. This study seeks to identify gene expression using microRNA and RNA sequencing in whole-blood samples from experienced breachers and unexposed controls. We performed experimental RNA sequencing using Illumina’s HiSeq 2500 Sequencing System, and microRNA analysis using NanoString Technology nCounter miRNA expression panel in whole-blood total RNA samples from 15 experienced breachers and 14 age-, sex-, and race-matched unexposed controls. We identified 10 significantly dysregulated genes between experienced breachers and unexposed controls, with FDR corrected <0.05: One upregulated gene, <i>LINC00996</i> (<i>long intergenic non-protein coding RNA 996</i>); and nine downregulated genes, <i>IGLV3-16</i> (<i>immunoglobulin lambda variable 3-16</i>), <i>CD200</i> (<i>CD200 molecule</i>), <i>LILRB5</i> (<i>leukocyte immunoglobulin-like receptor B5</i>), <i>ZNF667-AS1</i> (<i>ZNF667 antisense RNA 1</i>), <i>LMOD1</i> (<i>leiomodin 1</i>), <i>CNTNAP2</i> (<i>contactin-associated protein 2</i>), <i>EVPL</i> (<i>envoplakin</i>), <i>DPF3</i> (<i>double PHD fingers 3</i>), and <i>IGHV4-34</i> (<i>immunoglobulin heavy variable 4-34</i>). The dysregulated gene expressions reported here have been associated with chronic inflammation and immune response, suggesting that these pathways may relate to the risk of lasting neurological symptoms following high exposures to blast over a career.https://www.mdpi.com/2227-9059/10/3/690repetitive low-level blastexperienced breachertraumatic brain injury |
| spellingShingle | Rany Vorn Katie A. Edwards James Hentig Sijung Yun Hyung-Suk Kim Chen Lai Christina Devoto Angela M. Yarnell Elena Polejaeva Kristine C. Dell Matthew L. LoPresti Peter Walker Walter Carr James R. Stone Stephen T. Ahlers Jessica M. Gill A Pilot Study of Whole-Blood Transcriptomic Analysis to Identify Genes Associated with Repetitive Low-Level Blast Exposure in Career Breachers Biomedicines repetitive low-level blast experienced breacher traumatic brain injury |
| title | A Pilot Study of Whole-Blood Transcriptomic Analysis to Identify Genes Associated with Repetitive Low-Level Blast Exposure in Career Breachers |
| title_full | A Pilot Study of Whole-Blood Transcriptomic Analysis to Identify Genes Associated with Repetitive Low-Level Blast Exposure in Career Breachers |
| title_fullStr | A Pilot Study of Whole-Blood Transcriptomic Analysis to Identify Genes Associated with Repetitive Low-Level Blast Exposure in Career Breachers |
| title_full_unstemmed | A Pilot Study of Whole-Blood Transcriptomic Analysis to Identify Genes Associated with Repetitive Low-Level Blast Exposure in Career Breachers |
| title_short | A Pilot Study of Whole-Blood Transcriptomic Analysis to Identify Genes Associated with Repetitive Low-Level Blast Exposure in Career Breachers |
| title_sort | pilot study of whole blood transcriptomic analysis to identify genes associated with repetitive low level blast exposure in career breachers |
| topic | repetitive low-level blast experienced breacher traumatic brain injury |
| url | https://www.mdpi.com/2227-9059/10/3/690 |
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