1,3,6-Trigalloylglucose: A Novel Potent Anti-<i>Helicobacter pylori</i> Adhesion Agent Derived from Aqueous Extracts of <i>Terminalia chebula</i> Retz
1,3,6-Trigalloylglucose is a natural compound that can be extracted from the aqueous extracts of ripe fruit of <i>Terminalia chebula</i> Retz, commonly known as “<i>Haritaki</i>”. The potential anti-<i>Helicobacter pylori</i> (HP) activity of this compound has not...
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| Format: | Article |
| Language: | English |
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MDPI AG
2024-03-01
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| Series: | Molecules |
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| Online Access: | https://www.mdpi.com/1420-3049/29/5/1161 |
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| author | Ling Ou Zhixiang Zhu Yajie Hao Qingwei Li Hengrui Liu Qingchang Chen Chang Peng Chuqiu Zhang Yuanjing Zou Junwei Jia Hui Li Yanhua Wang Bingmei Su Yuqian Lai Meiyun Chen Haobo Chen Zhong Feng Guimin Zhang Meicun Yao |
| author_facet | Ling Ou Zhixiang Zhu Yajie Hao Qingwei Li Hengrui Liu Qingchang Chen Chang Peng Chuqiu Zhang Yuanjing Zou Junwei Jia Hui Li Yanhua Wang Bingmei Su Yuqian Lai Meiyun Chen Haobo Chen Zhong Feng Guimin Zhang Meicun Yao |
| author_sort | Ling Ou |
| collection | DOAJ |
| description | 1,3,6-Trigalloylglucose is a natural compound that can be extracted from the aqueous extracts of ripe fruit of <i>Terminalia chebula</i> Retz, commonly known as “<i>Haritaki</i>”. The potential anti-<i>Helicobacter pylori</i> (HP) activity of this compound has not been extensively studied or confirmed in scientific research. This compound was isolated using a semi-preparative liquid chromatography (LC) system and identified through Ultra-high-performance liquid chromatography–MS/MS (UPLC-MS/MS) and Nuclear Magnetic Resonance (NMR). Its role was evaluated using Minimum inhibitory concentration (MIC) assay and minimum bactericidal concentration (MBC) assay, scanning electron microscope (SEM), inhibiting kinetics curves, urea fast test, Cell Counting Kit-8 (CCK-8) assay, Western blot, and Griess Reagent System. Results showed that this compound effectively inhibits the growth of HP strain ATCC 700392, damages the HP structure, and suppresses the Cytotoxin-associated gene A (Cag A) protein, a crucial factor in HP infection. Importantly, it exhibits selective antimicrobial activity without impacting normal epithelial cells GES-1. In vitro studies have revealed that 1,3,6-Trigalloylglucose acts as an anti-adhesive agent, disrupting the adhesion of HP to host cells, a critical step in HP infection. These findings underscore the potential of 1,3,6-Trigalloylglucose as a targeted therapeutic agent against HP infections. |
| first_indexed | 2024-04-25T00:23:36Z |
| format | Article |
| id | doaj.art-a3bc8eafe84744128af6d119502f0463 |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-04-25T00:23:36Z |
| publishDate | 2024-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-a3bc8eafe84744128af6d119502f04632024-03-12T16:51:20ZengMDPI AGMolecules1420-30492024-03-01295116110.3390/molecules290511611,3,6-Trigalloylglucose: A Novel Potent Anti-<i>Helicobacter pylori</i> Adhesion Agent Derived from Aqueous Extracts of <i>Terminalia chebula</i> RetzLing Ou0Zhixiang Zhu1Yajie Hao2Qingwei Li3Hengrui Liu4Qingchang Chen5Chang Peng6Chuqiu Zhang7Yuanjing Zou8Junwei Jia9Hui Li10Yanhua Wang11Bingmei Su12Yuqian Lai13Meiyun Chen14Haobo Chen15Zhong Feng16Guimin Zhang17Meicun Yao18School of Pharmaceutical Sciences (Shenzhen), Sun Yat-sen University, Shenzhen 518107, ChinaSchool of Medicine and Pharmacy (Qingdao), Ocean University of China, Qingdao 266003, ChinaInternational Pharmaceutical Engineering Lab of Shandong Province, Linyi 273400, ChinaInternational Pharmaceutical Engineering Lab of Shandong Province, Linyi 273400, ChinaRegenerative Medicine Research Center, Future Homo Sapiens Institute of Regenerative Medicine Co., Ltd., Guangzhou 510535, ChinaDepartment of Biomedical Engineering, College of Design and Engineering, National University of Singapore, Singapore 117575, SingaporeSchool of Pharmaceutical Sciences (Shenzhen), Sun Yat-sen University, Shenzhen 518107, ChinaSchool of Pharmaceutical Sciences (Shenzhen), Sun Yat-sen University, Shenzhen 518107, ChinaSchool of Pharmaceutical Sciences (Shenzhen), Sun Yat-sen University, Shenzhen 518107, ChinaInternational Pharmaceutical Engineering Lab of Shandong Province, Linyi 273400, ChinaInternational Pharmaceutical Engineering Lab of Shandong Province, Linyi 273400, ChinaInternational Pharmaceutical Engineering Lab of Shandong Province, Linyi 273400, ChinaSchool of Pharmaceutical Sciences (Shenzhen), Sun Yat-sen University, Shenzhen 518107, ChinaSchool of Pharmaceutical Sciences (Shenzhen), Sun Yat-sen University, Shenzhen 518107, ChinaSchool of Pharmaceutical Sciences (Shenzhen), Sun Yat-sen University, Shenzhen 518107, ChinaSchool of Pharmaceutical Sciences (Shenzhen), Sun Yat-sen University, Shenzhen 518107, ChinaSchool of Pharmaceutical Sciences (Shenzhen), Sun Yat-sen University, Shenzhen 518107, ChinaLunan Pharmaceutical Group Co., Ltd., Linyi 276006, ChinaSchool of Pharmaceutical Sciences (Shenzhen), Sun Yat-sen University, Shenzhen 518107, China1,3,6-Trigalloylglucose is a natural compound that can be extracted from the aqueous extracts of ripe fruit of <i>Terminalia chebula</i> Retz, commonly known as “<i>Haritaki</i>”. The potential anti-<i>Helicobacter pylori</i> (HP) activity of this compound has not been extensively studied or confirmed in scientific research. This compound was isolated using a semi-preparative liquid chromatography (LC) system and identified through Ultra-high-performance liquid chromatography–MS/MS (UPLC-MS/MS) and Nuclear Magnetic Resonance (NMR). Its role was evaluated using Minimum inhibitory concentration (MIC) assay and minimum bactericidal concentration (MBC) assay, scanning electron microscope (SEM), inhibiting kinetics curves, urea fast test, Cell Counting Kit-8 (CCK-8) assay, Western blot, and Griess Reagent System. Results showed that this compound effectively inhibits the growth of HP strain ATCC 700392, damages the HP structure, and suppresses the Cytotoxin-associated gene A (Cag A) protein, a crucial factor in HP infection. Importantly, it exhibits selective antimicrobial activity without impacting normal epithelial cells GES-1. In vitro studies have revealed that 1,3,6-Trigalloylglucose acts as an anti-adhesive agent, disrupting the adhesion of HP to host cells, a critical step in HP infection. These findings underscore the potential of 1,3,6-Trigalloylglucose as a targeted therapeutic agent against HP infections.https://www.mdpi.com/1420-3049/29/5/11611,3,6-Trigalloylglucose<i>Terminalia chebula</i> Retz<i>Helicobacter pylori</i>adhesionminimum inhibitory concentration |
| spellingShingle | Ling Ou Zhixiang Zhu Yajie Hao Qingwei Li Hengrui Liu Qingchang Chen Chang Peng Chuqiu Zhang Yuanjing Zou Junwei Jia Hui Li Yanhua Wang Bingmei Su Yuqian Lai Meiyun Chen Haobo Chen Zhong Feng Guimin Zhang Meicun Yao 1,3,6-Trigalloylglucose: A Novel Potent Anti-<i>Helicobacter pylori</i> Adhesion Agent Derived from Aqueous Extracts of <i>Terminalia chebula</i> Retz Molecules 1,3,6-Trigalloylglucose <i>Terminalia chebula</i> Retz <i>Helicobacter pylori</i> adhesion minimum inhibitory concentration |
| title | 1,3,6-Trigalloylglucose: A Novel Potent Anti-<i>Helicobacter pylori</i> Adhesion Agent Derived from Aqueous Extracts of <i>Terminalia chebula</i> Retz |
| title_full | 1,3,6-Trigalloylglucose: A Novel Potent Anti-<i>Helicobacter pylori</i> Adhesion Agent Derived from Aqueous Extracts of <i>Terminalia chebula</i> Retz |
| title_fullStr | 1,3,6-Trigalloylglucose: A Novel Potent Anti-<i>Helicobacter pylori</i> Adhesion Agent Derived from Aqueous Extracts of <i>Terminalia chebula</i> Retz |
| title_full_unstemmed | 1,3,6-Trigalloylglucose: A Novel Potent Anti-<i>Helicobacter pylori</i> Adhesion Agent Derived from Aqueous Extracts of <i>Terminalia chebula</i> Retz |
| title_short | 1,3,6-Trigalloylglucose: A Novel Potent Anti-<i>Helicobacter pylori</i> Adhesion Agent Derived from Aqueous Extracts of <i>Terminalia chebula</i> Retz |
| title_sort | 1 3 6 trigalloylglucose a novel potent anti i helicobacter pylori i adhesion agent derived from aqueous extracts of i terminalia chebula i retz |
| topic | 1,3,6-Trigalloylglucose <i>Terminalia chebula</i> Retz <i>Helicobacter pylori</i> adhesion minimum inhibitory concentration |
| url | https://www.mdpi.com/1420-3049/29/5/1161 |
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