Simultaneous Quantification of Steroid Hormones Using hrLC-MS in Endocrine Tissues of Male Rats and Human Samples
Steroid hormones play a vital role in the regulation of cellular processes, and dysregulation of these metabolites can provoke or aggravate pathological issues, such as autoimmune diseases and cancer. Regulation of steroid hormones involves different organs and biological compartments. Therefore, it...
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| Format: | Article |
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MDPI AG
2022-07-01
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| Series: | Metabolites |
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| Online Access: | https://www.mdpi.com/2218-1989/12/8/714 |
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| author | Guillermo Bordanaba-Florit Sebastiaan van Liempd Diana Cabrera Félix Royo Juan Manuel Falcón-Pérez |
| author_facet | Guillermo Bordanaba-Florit Sebastiaan van Liempd Diana Cabrera Félix Royo Juan Manuel Falcón-Pérez |
| author_sort | Guillermo Bordanaba-Florit |
| collection | DOAJ |
| description | Steroid hormones play a vital role in the regulation of cellular processes, and dysregulation of these metabolites can provoke or aggravate pathological issues, such as autoimmune diseases and cancer. Regulation of steroid hormones involves different organs and biological compartments. Therefore, it is important to accurately determine their levels in tissues and biofluids to monitor changes after challenge or during disease. In this work, we have developed and optimized the extraction and quantification of 11 key members of the different steroid classes, including androgens, estrogens, progestogens and corticoids. The assay consists of a liquid/liquid extraction step and subsequent quantification by high-resolution liquid chromatography coupled time-of-flight mass spectrometry. The recoveries range between 74.2 to 126.9% and 54.9 to 110.7%, using a cell culture or urine as matrix, respectively. In general, the signal intensity loss due to matrix effect is no more than 30%. The method has been tested in relevant steroidogenic tissues in rat models and it has also been tested in human urine samples. Overall, this assay measures 11 analytes simultaneously in 6 min runtime and it has been applied in adrenal gland, testis, prostate, brain and serum from rats, and urine and extracellular vesicles from humans. |
| first_indexed | 2024-03-09T04:06:49Z |
| format | Article |
| id | doaj.art-a3bf3ab77fdd4536b1b15e95ebc5411e |
| institution | Directory Open Access Journal |
| issn | 2218-1989 |
| language | English |
| last_indexed | 2024-03-09T04:06:49Z |
| publishDate | 2022-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Metabolites |
| spelling | doaj.art-a3bf3ab77fdd4536b1b15e95ebc5411e2023-12-03T14:06:12ZengMDPI AGMetabolites2218-19892022-07-0112871410.3390/metabo12080714Simultaneous Quantification of Steroid Hormones Using hrLC-MS in Endocrine Tissues of Male Rats and Human SamplesGuillermo Bordanaba-Florit0Sebastiaan van Liempd1Diana Cabrera2Félix Royo3Juan Manuel Falcón-Pérez4Exosomes Laboratory, Center for Cooperative Research in Biosciences (CIC bioGUNE), Basque Research and Technology Alliance (BRTA), 48160 Derio, SpainMetabolomics Platform, Center for Cooperative Research in Biosciences (CIC bioGUNE), Basque Research and Technology Alliance (BRTA), 48160 Derio, SpainMetabolomics Platform, Center for Cooperative Research in Biosciences (CIC bioGUNE), Basque Research and Technology Alliance (BRTA), 48160 Derio, SpainExosomes Laboratory, Center for Cooperative Research in Biosciences (CIC bioGUNE), Basque Research and Technology Alliance (BRTA), 48160 Derio, SpainExosomes Laboratory, Center for Cooperative Research in Biosciences (CIC bioGUNE), Basque Research and Technology Alliance (BRTA), 48160 Derio, SpainSteroid hormones play a vital role in the regulation of cellular processes, and dysregulation of these metabolites can provoke or aggravate pathological issues, such as autoimmune diseases and cancer. Regulation of steroid hormones involves different organs and biological compartments. Therefore, it is important to accurately determine their levels in tissues and biofluids to monitor changes after challenge or during disease. In this work, we have developed and optimized the extraction and quantification of 11 key members of the different steroid classes, including androgens, estrogens, progestogens and corticoids. The assay consists of a liquid/liquid extraction step and subsequent quantification by high-resolution liquid chromatography coupled time-of-flight mass spectrometry. The recoveries range between 74.2 to 126.9% and 54.9 to 110.7%, using a cell culture or urine as matrix, respectively. In general, the signal intensity loss due to matrix effect is no more than 30%. The method has been tested in relevant steroidogenic tissues in rat models and it has also been tested in human urine samples. Overall, this assay measures 11 analytes simultaneously in 6 min runtime and it has been applied in adrenal gland, testis, prostate, brain and serum from rats, and urine and extracellular vesicles from humans.https://www.mdpi.com/2218-1989/12/8/714liquid chromatography–mass spectrometrytime-of-flightsteroid hormonesandrogensurinary extracellular vesicleshormone-dependent disease |
| spellingShingle | Guillermo Bordanaba-Florit Sebastiaan van Liempd Diana Cabrera Félix Royo Juan Manuel Falcón-Pérez Simultaneous Quantification of Steroid Hormones Using hrLC-MS in Endocrine Tissues of Male Rats and Human Samples Metabolites liquid chromatography–mass spectrometry time-of-flight steroid hormones androgens urinary extracellular vesicles hormone-dependent disease |
| title | Simultaneous Quantification of Steroid Hormones Using hrLC-MS in Endocrine Tissues of Male Rats and Human Samples |
| title_full | Simultaneous Quantification of Steroid Hormones Using hrLC-MS in Endocrine Tissues of Male Rats and Human Samples |
| title_fullStr | Simultaneous Quantification of Steroid Hormones Using hrLC-MS in Endocrine Tissues of Male Rats and Human Samples |
| title_full_unstemmed | Simultaneous Quantification of Steroid Hormones Using hrLC-MS in Endocrine Tissues of Male Rats and Human Samples |
| title_short | Simultaneous Quantification of Steroid Hormones Using hrLC-MS in Endocrine Tissues of Male Rats and Human Samples |
| title_sort | simultaneous quantification of steroid hormones using hrlc ms in endocrine tissues of male rats and human samples |
| topic | liquid chromatography–mass spectrometry time-of-flight steroid hormones androgens urinary extracellular vesicles hormone-dependent disease |
| url | https://www.mdpi.com/2218-1989/12/8/714 |
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