Salicylic Acid Improves the Constitutive Freezing Tolerance of Potato as Revealed by Transcriptomics and Metabolomics Analyses
Freezing severely impacts potato production. Deciphering the pathways and metabolites that regulate the freezing tolerance of potato is useful in cultivation and breeding for hardiness. In the present study, <i>Solanum acaule</i> was identified to be more freezing tolerant than <i>...
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MDPI AG
2022-12-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/24/1/609 |
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| author | Lin Chen Feiyan Zhou Ye Chen Yongqi Fan Kangkang Zhang Qing Liu Wei Tu Fujing Jiang Guangcun Li Hongbo Zhao Botao Song |
| author_facet | Lin Chen Feiyan Zhou Ye Chen Yongqi Fan Kangkang Zhang Qing Liu Wei Tu Fujing Jiang Guangcun Li Hongbo Zhao Botao Song |
| author_sort | Lin Chen |
| collection | DOAJ |
| description | Freezing severely impacts potato production. Deciphering the pathways and metabolites that regulate the freezing tolerance of potato is useful in cultivation and breeding for hardiness. In the present study, <i>Solanum acaule</i> was identified to be more freezing tolerant than <i>S. tuberosum</i>. Furthermore, the two genotypes before/after exposure to 4 °C for 7 d with additional −1 °C for 12 h were analysed by RNA-seq and metabolomics, and the results were compared with the previous −1 °C for 12 h. The results showed that <i>S. acaule</i> activated numerous genes that differed from those of <i>S. tuberosum</i>. Among the genes, five pathways, such as the hormone signalling pathway, which includes salicylic acid, were enriched. Further metabolomics analysis showed that the content of salicylic acid was improved in <i>S. acaule</i> in response to −1 °C for 12 h. Moreover, exogenous application of 0.1 mM salicylic acid to potato was shown to improve constitutive freezing tolerance and increase the expression of <i>HSFC1</i>. Following transcriptome and metabolome analyses, it was documented that the content of SA that increased in freezing-tolerant <i>S. acaule</i> after exposure to cold condition, associated with the SA signalling pathway, enhanced potato freezing tolerance, probably through <i>HSFC1</i>. |
| first_indexed | 2024-03-11T09:59:07Z |
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| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-11T09:59:07Z |
| publishDate | 2022-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-a3c6b94f23064ea9b7f27d765abe5f002023-11-16T15:35:52ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672022-12-0124160910.3390/ijms24010609Salicylic Acid Improves the Constitutive Freezing Tolerance of Potato as Revealed by Transcriptomics and Metabolomics AnalysesLin Chen0Feiyan Zhou1Ye Chen2Yongqi Fan3Kangkang Zhang4Qing Liu5Wei Tu6Fujing Jiang7Guangcun Li8Hongbo Zhao9Botao Song10Key Laboratory of Biology and Genetic Improvement of Tuber and Root Crop, Ministry of Agriculture and Rural Affairs, South China Agricultural University, Guangzhou 510642, ChinaKey Laboratory of Biology and Genetic Improvement of Tuber and Root Crop, Ministry of Agriculture and Rural Affairs, South China Agricultural University, Guangzhou 510642, ChinaKey Laboratory of Horticultural Plant Biology, Ministry of Education, Huazhong Agricultural University, Wuhan 430070, ChinaKey Laboratory of Biology and Genetic Improvement of Tuber and Root Crop, Ministry of Agriculture and Rural Affairs, South China Agricultural University, Guangzhou 510642, ChinaKey Laboratory of Biology and Genetic Improvement of Tuber and Root Crop, Ministry of Agriculture and Rural Affairs, South China Agricultural University, Guangzhou 510642, ChinaKey Laboratory of Biology and Genetic Improvement of Tuber and Root Crop, Ministry of Agriculture and Rural Affairs, South China Agricultural University, Guangzhou 510642, ChinaKey Laboratory of Horticultural Plant Biology, Ministry of Education, Huazhong Agricultural University, Wuhan 430070, ChinaKey Laboratory of Biology and Genetic Improvement of Tuber and Root Crop, Ministry of Agriculture and Rural Affairs, South China Agricultural University, Guangzhou 510642, ChinaKey Laboratory of Biology and Genetic Improvement of Tuber and Root Crop, Ministry of Agriculture and Rural Affairs, South China Agricultural University, Guangzhou 510642, ChinaKey Laboratory of Biology and Genetic Improvement of Tuber and Root Crop, Ministry of Agriculture and Rural Affairs, South China Agricultural University, Guangzhou 510642, ChinaKey Laboratory of Horticultural Plant Biology, Ministry of Education, Huazhong Agricultural University, Wuhan 430070, ChinaFreezing severely impacts potato production. Deciphering the pathways and metabolites that regulate the freezing tolerance of potato is useful in cultivation and breeding for hardiness. In the present study, <i>Solanum acaule</i> was identified to be more freezing tolerant than <i>S. tuberosum</i>. Furthermore, the two genotypes before/after exposure to 4 °C for 7 d with additional −1 °C for 12 h were analysed by RNA-seq and metabolomics, and the results were compared with the previous −1 °C for 12 h. The results showed that <i>S. acaule</i> activated numerous genes that differed from those of <i>S. tuberosum</i>. Among the genes, five pathways, such as the hormone signalling pathway, which includes salicylic acid, were enriched. Further metabolomics analysis showed that the content of salicylic acid was improved in <i>S. acaule</i> in response to −1 °C for 12 h. Moreover, exogenous application of 0.1 mM salicylic acid to potato was shown to improve constitutive freezing tolerance and increase the expression of <i>HSFC1</i>. Following transcriptome and metabolome analyses, it was documented that the content of SA that increased in freezing-tolerant <i>S. acaule</i> after exposure to cold condition, associated with the SA signalling pathway, enhanced potato freezing tolerance, probably through <i>HSFC1</i>.https://www.mdpi.com/1422-0067/24/1/609<i>Solanum acaule</i><i>Solanum tuberosum</i>acclimated freezing toleranceCBF<i>HSFC1</i> |
| spellingShingle | Lin Chen Feiyan Zhou Ye Chen Yongqi Fan Kangkang Zhang Qing Liu Wei Tu Fujing Jiang Guangcun Li Hongbo Zhao Botao Song Salicylic Acid Improves the Constitutive Freezing Tolerance of Potato as Revealed by Transcriptomics and Metabolomics Analyses International Journal of Molecular Sciences <i>Solanum acaule</i> <i>Solanum tuberosum</i> acclimated freezing tolerance CBF <i>HSFC1</i> |
| title | Salicylic Acid Improves the Constitutive Freezing Tolerance of Potato as Revealed by Transcriptomics and Metabolomics Analyses |
| title_full | Salicylic Acid Improves the Constitutive Freezing Tolerance of Potato as Revealed by Transcriptomics and Metabolomics Analyses |
| title_fullStr | Salicylic Acid Improves the Constitutive Freezing Tolerance of Potato as Revealed by Transcriptomics and Metabolomics Analyses |
| title_full_unstemmed | Salicylic Acid Improves the Constitutive Freezing Tolerance of Potato as Revealed by Transcriptomics and Metabolomics Analyses |
| title_short | Salicylic Acid Improves the Constitutive Freezing Tolerance of Potato as Revealed by Transcriptomics and Metabolomics Analyses |
| title_sort | salicylic acid improves the constitutive freezing tolerance of potato as revealed by transcriptomics and metabolomics analyses |
| topic | <i>Solanum acaule</i> <i>Solanum tuberosum</i> acclimated freezing tolerance CBF <i>HSFC1</i> |
| url | https://www.mdpi.com/1422-0067/24/1/609 |
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