High-Resolution 3D Reconstruction of Human Oocytes Using Focused Ion Beam Scanning Electron Microscopy

High-Resolution 3D Reconstruction of Human Oocytes Using Focused Ion Beam Scanning Electron Microscopy

The egg plays a pivotal role in the reproduction of our species. Nevertheless, its fundamental biology remains elusive. Transmission electron microscopy is traditionally used to inspect the ultrastructure of female gametes. However, two-dimensional micrographs contain only fragmentary information ab...

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Bibliographic Details
Main Authors: Zuzana Trebichalská, Jakub Javůrek, Martina Tatíčková, Drahomíra Kyjovská, Soňa Kloudová, Pavel Otevřel, Aleš Hampl, Zuzana Holubcová
Format: Article
Language:English
Published: Frontiers Media S.A. 2021-11-01
Series:Frontiers in Cell and Developmental Biology
Subjects:
electron microscopy
FIB-SEM
volume microscopy
3D ultrastructure
human oocyte
oocyte maturation
Online Access:https://www.frontiersin.org/articles/10.3389/fcell.2021.755740/full

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