Ultrasensitive detection of praziquantel in sea bass (Lateolabrax japonicus) using a lateral flow immunochromatographic assay
Praziquantel (PZQ) is a compound that usually used as insecticide, drug residue could be harm to human health. In this study, we have developed a simple, sensitive, and rapid lateral flow immunochromatographic assay (LFIA) for the detection of PZQ in fish (Lateolabrax Japonicus). We produced a sensi...
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2020-01-01
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| Series: | Food and Agricultural Immunology |
| Subjects: | |
| Online Access: | http://dx.doi.org/10.1080/09540105.2020.1776685 |
| _version_ | 1818665500507373568 |
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| author | Kai Hao Steven Suryoprabowo Hua Kuang Shanshan Song |
| author_facet | Kai Hao Steven Suryoprabowo Hua Kuang Shanshan Song |
| author_sort | Kai Hao |
| collection | DOAJ |
| description | Praziquantel (PZQ) is a compound that usually used as insecticide, drug residue could be harm to human health. In this study, we have developed a simple, sensitive, and rapid lateral flow immunochromatographic assay (LFIA) for the detection of PZQ in fish (Lateolabrax Japonicus). We produced a sensitive mAb using enzyme-linked immunosorbent assay with half-maximal inhibitory concentration (2A11, IC50) of 2.06 ng/mL. The coating antigen (PZQ-HS-GA-OVA) and goat anti-mouse IgG antibody were sprayed onto a nitrocellulose membrane as the test and control lines, respectively. Under optimal conditions, the cut-off limits for PZQ detection on the test strips were determined to be 5 ng/mL in both 0.01 M phosphate buffered saline and fish samples, and results were obtained within 5 min. Our data suggests that this LFIA can be used for the sensitive, rapid, and specific on-site screening for PZQ in fish. |
| first_indexed | 2024-12-17T05:49:37Z |
| format | Article |
| id | doaj.art-a3ca5f596d0a49babdf87752723e9cf9 |
| institution | Directory Open Access Journal |
| issn | 0954-0105 1465-3443 |
| language | English |
| last_indexed | 2024-12-17T05:49:37Z |
| publishDate | 2020-01-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Food and Agricultural Immunology |
| spelling | doaj.art-a3ca5f596d0a49babdf87752723e9cf92022-12-21T22:01:12ZengTaylor & Francis GroupFood and Agricultural Immunology0954-01051465-34432020-01-0131177878810.1080/09540105.2020.17766851776685Ultrasensitive detection of praziquantel in sea bass (Lateolabrax japonicus) using a lateral flow immunochromatographic assayKai Hao0Steven Suryoprabowo1Hua Kuang2Shanshan Song3School of Agricultural Engineering and Food Science, Shandong University of TechnologyState Key Laboratory of Food Science and Technology, Jiangnan UniversityState Key Laboratory of Food Science and Technology, Jiangnan UniversityState Key Laboratory of Food Science and Technology, Jiangnan UniversityPraziquantel (PZQ) is a compound that usually used as insecticide, drug residue could be harm to human health. In this study, we have developed a simple, sensitive, and rapid lateral flow immunochromatographic assay (LFIA) for the detection of PZQ in fish (Lateolabrax Japonicus). We produced a sensitive mAb using enzyme-linked immunosorbent assay with half-maximal inhibitory concentration (2A11, IC50) of 2.06 ng/mL. The coating antigen (PZQ-HS-GA-OVA) and goat anti-mouse IgG antibody were sprayed onto a nitrocellulose membrane as the test and control lines, respectively. Under optimal conditions, the cut-off limits for PZQ detection on the test strips were determined to be 5 ng/mL in both 0.01 M phosphate buffered saline and fish samples, and results were obtained within 5 min. Our data suggests that this LFIA can be used for the sensitive, rapid, and specific on-site screening for PZQ in fish.http://dx.doi.org/10.1080/09540105.2020.1776685lateral flow immunochromatography assaymonoclonal antibodypraziquantelstrip test (lateolabrax japonicus) |
| spellingShingle | Kai Hao Steven Suryoprabowo Hua Kuang Shanshan Song Ultrasensitive detection of praziquantel in sea bass (Lateolabrax japonicus) using a lateral flow immunochromatographic assay Food and Agricultural Immunology lateral flow immunochromatography assay monoclonal antibody praziquantel strip test (lateolabrax japonicus) |
| title | Ultrasensitive detection of praziquantel in sea bass (Lateolabrax japonicus) using a lateral flow immunochromatographic assay |
| title_full | Ultrasensitive detection of praziquantel in sea bass (Lateolabrax japonicus) using a lateral flow immunochromatographic assay |
| title_fullStr | Ultrasensitive detection of praziquantel in sea bass (Lateolabrax japonicus) using a lateral flow immunochromatographic assay |
| title_full_unstemmed | Ultrasensitive detection of praziquantel in sea bass (Lateolabrax japonicus) using a lateral flow immunochromatographic assay |
| title_short | Ultrasensitive detection of praziquantel in sea bass (Lateolabrax japonicus) using a lateral flow immunochromatographic assay |
| title_sort | ultrasensitive detection of praziquantel in sea bass lateolabrax japonicus using a lateral flow immunochromatographic assay |
| topic | lateral flow immunochromatography assay monoclonal antibody praziquantel strip test (lateolabrax japonicus) |
| url | http://dx.doi.org/10.1080/09540105.2020.1776685 |
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