Iron accumulation deteriorated bone loss in estrogen-deficient rats
Abstract Background Postmenopausal osteoporosis is characterized by an imbalance of bone resorption exceeding bone formation, resulting in a net loss of bone mass. Whether a menopause-related excess of iron contributes to the development of postmenopausal osteoporosis has remained unresolved due to...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2021-08-01
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| Series: | Journal of Orthopaedic Surgery and Research |
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| Online Access: | https://doi.org/10.1186/s13018-021-02663-4 |
| _version_ | 1798025237299724288 |
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| author | Lu-lin Liu Gong-wen Liu Hui Liu Kai Zhao You-jia Xu |
| author_facet | Lu-lin Liu Gong-wen Liu Hui Liu Kai Zhao You-jia Xu |
| author_sort | Lu-lin Liu |
| collection | DOAJ |
| description | Abstract Background Postmenopausal osteoporosis is characterized by an imbalance of bone resorption exceeding bone formation, resulting in a net loss of bone mass. Whether a menopause-related excess of iron contributes to the development of postmenopausal osteoporosis has remained unresolved due to a lack of an appropriate animal model. This study aimed to explore the effects of iron accumulation in bone mass in estrogen-deficient rats. Methods In the present study, ovariectomy (OVX) was performed in female rats and the changes of iron metabolism and some related modulated genes were detected. Ferric ammonium citrate (FAC) was used as a donor of iron for OVX rats. Moreover, micro-CT was performed to assess the bone microarchitecture in sham group, OVX, and FAC groups. Histological detection of iron in liver was assessed by Perl’s staining. The expressions of β-CTX and osteocalcin were assessed by ELISA. Results It was found that serum iron decreased after OVX. It was found that the expressions of Hepcidin in liver and Fpn, DMT-1 in duodenum significantly decreased at transcriptional level in OVX group than sham group. However, no difference existed in the expression of DMT-1. Then, ferric ammonium citrate (FAC) was used as a donor of iron for OVX rats. The FAC group manifested significant iron accumulation by increased serum iron and hepatic iron content. In addition, FAC treatment accelerated bone loss and decreased BMD and biomechanics in OVX rats. Moreover, bone biomarker β-CTX rather than osteocalcin increased significantly in FAC groups than OVX group. Conclusions In conclusion, no iron accumulation occurred in OVX rats. Furthermore, iron accumulation could further deteriorate osteopenia through enhanced bone resorption. |
| first_indexed | 2024-04-11T18:15:31Z |
| format | Article |
| id | doaj.art-a3cd4422f8014485be00446158a874b0 |
| institution | Directory Open Access Journal |
| issn | 1749-799X |
| language | English |
| last_indexed | 2024-04-11T18:15:31Z |
| publishDate | 2021-08-01 |
| publisher | BMC |
| record_format | Article |
| series | Journal of Orthopaedic Surgery and Research |
| spelling | doaj.art-a3cd4422f8014485be00446158a874b02022-12-22T04:09:55ZengBMCJournal of Orthopaedic Surgery and Research1749-799X2021-08-011611810.1186/s13018-021-02663-4Iron accumulation deteriorated bone loss in estrogen-deficient ratsLu-lin Liu0Gong-wen Liu1Hui Liu2Kai Zhao3You-jia Xu4Department of Orthopedics, The First Affiliated Hospital of Gannan Medical UniversitySuzhou TCM Hospital Affiliated to Nanjing University of Chinese MedicineDepartment of Orthopedics, Ganxian District Traditional Chinese Medicine Hospital of Ganzhou CityDepartment of Orthopedics, The First Affiliated Hospital of Gannan Medical UniversityDepartment of Orthopedics, Second Affiliated Hospital of Soochow UniversityAbstract Background Postmenopausal osteoporosis is characterized by an imbalance of bone resorption exceeding bone formation, resulting in a net loss of bone mass. Whether a menopause-related excess of iron contributes to the development of postmenopausal osteoporosis has remained unresolved due to a lack of an appropriate animal model. This study aimed to explore the effects of iron accumulation in bone mass in estrogen-deficient rats. Methods In the present study, ovariectomy (OVX) was performed in female rats and the changes of iron metabolism and some related modulated genes were detected. Ferric ammonium citrate (FAC) was used as a donor of iron for OVX rats. Moreover, micro-CT was performed to assess the bone microarchitecture in sham group, OVX, and FAC groups. Histological detection of iron in liver was assessed by Perl’s staining. The expressions of β-CTX and osteocalcin were assessed by ELISA. Results It was found that serum iron decreased after OVX. It was found that the expressions of Hepcidin in liver and Fpn, DMT-1 in duodenum significantly decreased at transcriptional level in OVX group than sham group. However, no difference existed in the expression of DMT-1. Then, ferric ammonium citrate (FAC) was used as a donor of iron for OVX rats. The FAC group manifested significant iron accumulation by increased serum iron and hepatic iron content. In addition, FAC treatment accelerated bone loss and decreased BMD and biomechanics in OVX rats. Moreover, bone biomarker β-CTX rather than osteocalcin increased significantly in FAC groups than OVX group. Conclusions In conclusion, no iron accumulation occurred in OVX rats. Furthermore, iron accumulation could further deteriorate osteopenia through enhanced bone resorption.https://doi.org/10.1186/s13018-021-02663-4Iron overloadOvariectomized ratOsteoporosisMicro-CTIron metabolismBiomechanical testing |
| spellingShingle | Lu-lin Liu Gong-wen Liu Hui Liu Kai Zhao You-jia Xu Iron accumulation deteriorated bone loss in estrogen-deficient rats Journal of Orthopaedic Surgery and Research Iron overload Ovariectomized rat Osteoporosis Micro-CT Iron metabolism Biomechanical testing |
| title | Iron accumulation deteriorated bone loss in estrogen-deficient rats |
| title_full | Iron accumulation deteriorated bone loss in estrogen-deficient rats |
| title_fullStr | Iron accumulation deteriorated bone loss in estrogen-deficient rats |
| title_full_unstemmed | Iron accumulation deteriorated bone loss in estrogen-deficient rats |
| title_short | Iron accumulation deteriorated bone loss in estrogen-deficient rats |
| title_sort | iron accumulation deteriorated bone loss in estrogen deficient rats |
| topic | Iron overload Ovariectomized rat Osteoporosis Micro-CT Iron metabolism Biomechanical testing |
| url | https://doi.org/10.1186/s13018-021-02663-4 |
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