Alternative exon usage creates novel transcript variants of tumor suppressor SHREW-1 gene with differential tissue expression profile
Shrew-1, also called AJAP1, is a transmembrane protein associated with E-cadherin-mediated adherence junctions and a putative tumor suppressor. Apart from its interaction with β-catenin and involvement in E-cadherin internalization, little structure or function information exists. Here we explored s...
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| Format: | Article |
| Language: | English |
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The Company of Biologists
2016-11-01
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| Series: | Biology Open |
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| Online Access: | http://bio.biologists.org/content/5/11/1607 |
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| author | Petra A. B. Klemmt Eduard Resch Isabell Smyrek Knut Engels Ernst H. K. Stelzer Anna Starzinski-Powitz |
| author_facet | Petra A. B. Klemmt Eduard Resch Isabell Smyrek Knut Engels Ernst H. K. Stelzer Anna Starzinski-Powitz |
| author_sort | Petra A. B. Klemmt |
| collection | DOAJ |
| description | Shrew-1, also called AJAP1, is a transmembrane protein associated with E-cadherin-mediated adherence junctions and a putative tumor suppressor. Apart from its interaction with β-catenin and involvement in E-cadherin internalization, little structure or function information exists. Here we explored shrew-1 expression during postnatal differentiation of mammary gland as a model system. Immunohistological analyses with antibodies against either the extracellular or the cytoplasmic domains of shrew-1 consistently revealed the expression of full-length shrew-1 in myoepithelial cells, but only part of it in luminal cells. While shrew-1 localization remained unaltered in myoepithelial cells, nuclear localization occurred in luminal cells during lactation. Based on these observations, we identified two unknown shrew-1 transcript variants encoding N-terminally truncated proteins. The smallest shrew-1 protein lacks the extracellular domain and is most likely the only variant present in luminal cells. RNA analyses of human tissues confirmed that the novel transcript variants of shrew-1 exist in vivo and exhibit a differential tissue expression profile. We conclude that our findings are essential for the understanding and interpretation of future functional and interactome analyses of shrew-1 variants. |
| first_indexed | 2024-12-14T18:05:01Z |
| format | Article |
| id | doaj.art-a3cd5917bbd6477ba61ab1818a3e5295 |
| institution | Directory Open Access Journal |
| issn | 2046-6390 |
| language | English |
| last_indexed | 2024-12-14T18:05:01Z |
| publishDate | 2016-11-01 |
| publisher | The Company of Biologists |
| record_format | Article |
| series | Biology Open |
| spelling | doaj.art-a3cd5917bbd6477ba61ab1818a3e52952022-12-21T22:52:23ZengThe Company of BiologistsBiology Open2046-63902016-11-015111607161910.1242/bio.019463019463Alternative exon usage creates novel transcript variants of tumor suppressor SHREW-1 gene with differential tissue expression profilePetra A. B. Klemmt0Eduard Resch1Isabell Smyrek2Knut Engels3Ernst H. K. Stelzer4Anna Starzinski-Powitz5 Institute of Cell Biology and Neuroscience, Department of Molecular Cell Biology and Human Genetics, Goethe Universität Frankfurt am Main, Max-von-Laue-Straße 13, Frankfurt am Main D-60438, Germany Institute of Cell Biology and Neuroscience, Department of Molecular Cell Biology and Human Genetics, Goethe Universität Frankfurt am Main, Max-von-Laue-Straße 13, Frankfurt am Main D-60438, Germany Physical Biology Group, Buchmann Institute for Molecular Life Sciences (BMLS), Goethe Universität Frankfurt am Main, Max-von-Laue-Straße 15, Frankfurt am Main D-60438, Germany Center for Pathology, Cytology and Molecular Pathology, Neuss D-41462, Germany Physical Biology Group, Buchmann Institute for Molecular Life Sciences (BMLS), Goethe Universität Frankfurt am Main, Max-von-Laue-Straße 15, Frankfurt am Main D-60438, Germany Institute of Cell Biology and Neuroscience, Department of Molecular Cell Biology and Human Genetics, Goethe Universität Frankfurt am Main, Max-von-Laue-Straße 13, Frankfurt am Main D-60438, Germany Shrew-1, also called AJAP1, is a transmembrane protein associated with E-cadherin-mediated adherence junctions and a putative tumor suppressor. Apart from its interaction with β-catenin and involvement in E-cadherin internalization, little structure or function information exists. Here we explored shrew-1 expression during postnatal differentiation of mammary gland as a model system. Immunohistological analyses with antibodies against either the extracellular or the cytoplasmic domains of shrew-1 consistently revealed the expression of full-length shrew-1 in myoepithelial cells, but only part of it in luminal cells. While shrew-1 localization remained unaltered in myoepithelial cells, nuclear localization occurred in luminal cells during lactation. Based on these observations, we identified two unknown shrew-1 transcript variants encoding N-terminally truncated proteins. The smallest shrew-1 protein lacks the extracellular domain and is most likely the only variant present in luminal cells. RNA analyses of human tissues confirmed that the novel transcript variants of shrew-1 exist in vivo and exhibit a differential tissue expression profile. We conclude that our findings are essential for the understanding and interpretation of future functional and interactome analyses of shrew-1 variants.http://bio.biologists.org/content/5/11/1607AJAP1Mammary glandLactationSplice variantsAdherence junctionsTumor suppressor protein |
| spellingShingle | Petra A. B. Klemmt Eduard Resch Isabell Smyrek Knut Engels Ernst H. K. Stelzer Anna Starzinski-Powitz Alternative exon usage creates novel transcript variants of tumor suppressor SHREW-1 gene with differential tissue expression profile Biology Open AJAP1 Mammary gland Lactation Splice variants Adherence junctions Tumor suppressor protein |
| title | Alternative exon usage creates novel transcript variants of tumor suppressor SHREW-1 gene with differential tissue expression profile |
| title_full | Alternative exon usage creates novel transcript variants of tumor suppressor SHREW-1 gene with differential tissue expression profile |
| title_fullStr | Alternative exon usage creates novel transcript variants of tumor suppressor SHREW-1 gene with differential tissue expression profile |
| title_full_unstemmed | Alternative exon usage creates novel transcript variants of tumor suppressor SHREW-1 gene with differential tissue expression profile |
| title_short | Alternative exon usage creates novel transcript variants of tumor suppressor SHREW-1 gene with differential tissue expression profile |
| title_sort | alternative exon usage creates novel transcript variants of tumor suppressor shrew 1 gene with differential tissue expression profile |
| topic | AJAP1 Mammary gland Lactation Splice variants Adherence junctions Tumor suppressor protein |
| url | http://bio.biologists.org/content/5/11/1607 |
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