The Prevalence of Inducible Clindamycin Resistance Among Staphylococci in a Tertiary Care Hospital – A Study from the Garhwal Hills of Uttarakhand, India
ABSTRACT Objective: This study was undertaken to assess the frequency of the phenotypic expression of the inducible resistance to clindamycin which was due to the expression of the erm genes in various clinical isolates of the Staphylococcus species. Materials and Methods: This was a cross sect...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
JCDR Research and Publications Private Limited
2013-01-01
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| Series: | Journal of Clinical and Diagnostic Research |
| Subjects: | |
| Online Access: | https://jcdr.net/articles/PDF/2671/16-%204877_E(C)_PF1(M)_F(P)_PFA(MP)_OLF(P)_PF1(RP)_u.pdf |
| Summary: | ABSTRACT
Objective: This study was undertaken to assess the frequency
of the phenotypic expression of the inducible resistance to
clindamycin which was due to the expression of the erm genes
in various clinical isolates of the Staphylococcus species.
Materials and Methods: This was a cross sectional study
conducted in the Dept. of Microbiology and Immunology,
Veer Chandra Singh Garhwali Govt. Medical Sciences and
Research Institute, Srikot, Uttarakhand, from July 2010 to
December 2011. A total of 373 consecutive, non duplicate
strains of Staphylococci isolated from various clinical samples
like pus, wound swab, blood, urine and other body fluids, were
tested. The isolates which had a discordant resistance pattern
(clindamycin-sensitive and erythromycin-resistant) by Kirby
Bauer Disk Diffusion method were selected and subjected to the
D-test for inducible clindamycin resistance, as per the Clinical
and Laboratory Standards Institutes (CLSI) guidelines.
Results: Among the 373 clinical isolates of Staphylococci which
were studied, 134 isolates showed a discordant resistance pattern. Among these discordant strains, 45 (33.6%) isolates were
D-test positive, which had inducible clindamycin resistance
and belonged to the inducible macrolide lincosamide streptogramin-B phenotype (MLSBi). 89 (66.4%) isolates were D-test
negative and they belonged to the macrolide streptogramin
phenotype (MS). Among the MLSBi phenotypes, 6 (13.3%) isolates were methicillin-resistant Staphylococcus aureus (MRSA),
13 (28.9%) were Methicillin-sensitive S.aureus (MSSA) and 26
(57.8%) were coagulase negative staphylococci (CONS).
Conclusion: The D-test is a simple, effective and an important
method for the phenotypic detection of inducible clindamycin
resistance and it should be used routinely, as it will help in
guiding the empirical therapy. The possible clinical failures can
thus be avoided. |
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| ISSN: | 2249-782X 0973-709X |