Transient transformation meets gene function discovery: the strawberry fruit case
Beside the well known nutritional and health benefits, strawberry (Fragaria X ananassa) crop draws increasing attention as plant model system for the Rosaceae family, due to the short generation time, the rapid in vitro regeneration, and to the availability of the genome sequence of F. X ananassa an...
| Main Authors: | , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2015-06-01
|
| Series: | Frontiers in Plant Science |
| Subjects: | |
| Online Access: | http://journal.frontiersin.org/Journal/10.3389/fpls.2015.00444/full |
| _version_ | 1811256285231841280 |
|---|---|
| author | Michela eGuidarelli Elena eBaraldi |
| author_facet | Michela eGuidarelli Elena eBaraldi |
| author_sort | Michela eGuidarelli |
| collection | DOAJ |
| description | Beside the well known nutritional and health benefits, strawberry (Fragaria X ananassa) crop draws increasing attention as plant model system for the Rosaceae family, due to the short generation time, the rapid in vitro regeneration, and to the availability of the genome sequence of F. X ananassa and of the closely related F. vesca species. In the last years, the use of high-throughput sequence technologies provided large amounts of molecular information on the genes possibly related to several biological processes of this crop. Nevertheless, the function of most genes or gene products is still poorly understood and needs investigation. Transient transformation technology provides a powerful tool to study gene function in vivo, avoiding difficult drawbacks that typically affect the stable transformation protocols, such as transformation efficiency, transformants selection and regeneration. In this review we provide an overview of the use of transient expression in the investigation of the function of genes important for strawberry fruit development, defence and nutritional properties. The technical aspects related to an efficient use of this technique are described, and the possible impact and application in strawberry crop improvement are discussed. |
| first_indexed | 2024-04-12T17:37:46Z |
| format | Article |
| id | doaj.art-a3d11f1d172c4d0e9f91f1103da9956f |
| institution | Directory Open Access Journal |
| issn | 1664-462X |
| language | English |
| last_indexed | 2024-04-12T17:37:46Z |
| publishDate | 2015-06-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Plant Science |
| spelling | doaj.art-a3d11f1d172c4d0e9f91f1103da9956f2022-12-22T03:22:54ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2015-06-01610.3389/fpls.2015.00444139872Transient transformation meets gene function discovery: the strawberry fruit caseMichela eGuidarelli0Elena eBaraldi1University of BolognaUniversity of BolognaBeside the well known nutritional and health benefits, strawberry (Fragaria X ananassa) crop draws increasing attention as plant model system for the Rosaceae family, due to the short generation time, the rapid in vitro regeneration, and to the availability of the genome sequence of F. X ananassa and of the closely related F. vesca species. In the last years, the use of high-throughput sequence technologies provided large amounts of molecular information on the genes possibly related to several biological processes of this crop. Nevertheless, the function of most genes or gene products is still poorly understood and needs investigation. Transient transformation technology provides a powerful tool to study gene function in vivo, avoiding difficult drawbacks that typically affect the stable transformation protocols, such as transformation efficiency, transformants selection and regeneration. In this review we provide an overview of the use of transient expression in the investigation of the function of genes important for strawberry fruit development, defence and nutritional properties. The technical aspects related to an efficient use of this technique are described, and the possible impact and application in strawberry crop improvement are discussed.http://journal.frontiersin.org/Journal/10.3389/fpls.2015.00444/fulloverexpressionstrawberryAgrobacterium-mediated transient transformationRNA-interferencegene function discovery |
| spellingShingle | Michela eGuidarelli Elena eBaraldi Transient transformation meets gene function discovery: the strawberry fruit case Frontiers in Plant Science overexpression strawberry Agrobacterium-mediated transient transformation RNA-interference gene function discovery |
| title | Transient transformation meets gene function discovery: the strawberry fruit case |
| title_full | Transient transformation meets gene function discovery: the strawberry fruit case |
| title_fullStr | Transient transformation meets gene function discovery: the strawberry fruit case |
| title_full_unstemmed | Transient transformation meets gene function discovery: the strawberry fruit case |
| title_short | Transient transformation meets gene function discovery: the strawberry fruit case |
| title_sort | transient transformation meets gene function discovery the strawberry fruit case |
| topic | overexpression strawberry Agrobacterium-mediated transient transformation RNA-interference gene function discovery |
| url | http://journal.frontiersin.org/Journal/10.3389/fpls.2015.00444/full |
| work_keys_str_mv | AT michelaeguidarelli transienttransformationmeetsgenefunctiondiscoverythestrawberryfruitcase AT elenaebaraldi transienttransformationmeetsgenefunctiondiscoverythestrawberryfruitcase |