Assessment of Choroidal Vasculature and Innate Immune Cells in the Eyes of Albino and Pigmented Mice
The visualization of choroidal vasculature and innate immune cells in the eyes of pigmented mice has been challenging due to the presence of a retinal pigment epithelium (RPE) layer separating the choroid and retina. Here, we established methods for visualizing the choroidal macrophages, mast cells,...
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MDPI AG
2022-10-01
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| Series: | Cells |
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| Online Access: | https://www.mdpi.com/2073-4409/11/20/3329 |
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| author | Ismail S. Zaitoun Yong-Seok Song Hammam B. Zaitoun Christine M. Sorenson Nader Sheibani |
| author_facet | Ismail S. Zaitoun Yong-Seok Song Hammam B. Zaitoun Christine M. Sorenson Nader Sheibani |
| author_sort | Ismail S. Zaitoun |
| collection | DOAJ |
| description | The visualization of choroidal vasculature and innate immune cells in the eyes of pigmented mice has been challenging due to the presence of a retinal pigment epithelium (RPE) layer separating the choroid and retina. Here, we established methods for visualizing the choroidal macrophages, mast cells, and vasculature in eyes of albino and pigmented mice using cell type-specific staining. We were able to visualize the choroidal arterial and venous systems. An arterial circle around the optic nerve was found in mice similar to the Zinn–Haller arterial circle that exists in humans and primates. Three different structural patterns of choriocapillaris were observed throughout the whole choroid: honeycomb-like, maze-like, and finger-like patterns. Choroidal mast cells were relatively few but dense around the optic nerve. Mast cell distribution in the middle and periphery was different among strains. Macrophages were found in all layers of the choroid. Thus, utilizing the simple and reliable methods described herein will allow the evaluation of transgenic and preclinical mouse models of ocular diseases that affect the choroid, including age-related macular degeneration (AMD), diabetic choroidopathy, and retinopathy of prematurity. These studies will advance our understanding of the pathophysiology, and molecular and cellular mechanisms that can be targeted therapeutically, in these diseases. |
| first_indexed | 2024-03-09T20:28:22Z |
| format | Article |
| id | doaj.art-a3d27e259e6b496d96a45a9540c124de |
| institution | Directory Open Access Journal |
| issn | 2073-4409 |
| language | English |
| last_indexed | 2024-03-09T20:28:22Z |
| publishDate | 2022-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Cells |
| spelling | doaj.art-a3d27e259e6b496d96a45a9540c124de2023-11-23T23:29:20ZengMDPI AGCells2073-44092022-10-011120332910.3390/cells11203329Assessment of Choroidal Vasculature and Innate Immune Cells in the Eyes of Albino and Pigmented MiceIsmail S. Zaitoun0Yong-Seok Song1Hammam B. Zaitoun2Christine M. Sorenson3Nader Sheibani4Department of Ophthalmology and Visual Sciences, School of Medicine and Public Health, University of Wisconsin, Madison, WI 53705, USADepartment of Ophthalmology and Visual Sciences, School of Medicine and Public Health, University of Wisconsin, Madison, WI 53705, USAFaculty of Medicine, Yarmouk University in Irbid, Irbid 21163, JordanMcPherson Eye Research Institute, School of Medicine and Public Health, University of Wisconsin, Madison, WI 53705, USADepartment of Ophthalmology and Visual Sciences, School of Medicine and Public Health, University of Wisconsin, Madison, WI 53705, USAThe visualization of choroidal vasculature and innate immune cells in the eyes of pigmented mice has been challenging due to the presence of a retinal pigment epithelium (RPE) layer separating the choroid and retina. Here, we established methods for visualizing the choroidal macrophages, mast cells, and vasculature in eyes of albino and pigmented mice using cell type-specific staining. We were able to visualize the choroidal arterial and venous systems. An arterial circle around the optic nerve was found in mice similar to the Zinn–Haller arterial circle that exists in humans and primates. Three different structural patterns of choriocapillaris were observed throughout the whole choroid: honeycomb-like, maze-like, and finger-like patterns. Choroidal mast cells were relatively few but dense around the optic nerve. Mast cell distribution in the middle and periphery was different among strains. Macrophages were found in all layers of the choroid. Thus, utilizing the simple and reliable methods described herein will allow the evaluation of transgenic and preclinical mouse models of ocular diseases that affect the choroid, including age-related macular degeneration (AMD), diabetic choroidopathy, and retinopathy of prematurity. These studies will advance our understanding of the pathophysiology, and molecular and cellular mechanisms that can be targeted therapeutically, in these diseases.https://www.mdpi.com/2073-4409/11/20/3329choriocapillarismast cellsmacrophagessmooth muscle cellsuveal diseasesage-related macular degeneration |
| spellingShingle | Ismail S. Zaitoun Yong-Seok Song Hammam B. Zaitoun Christine M. Sorenson Nader Sheibani Assessment of Choroidal Vasculature and Innate Immune Cells in the Eyes of Albino and Pigmented Mice Cells choriocapillaris mast cells macrophages smooth muscle cells uveal diseases age-related macular degeneration |
| title | Assessment of Choroidal Vasculature and Innate Immune Cells in the Eyes of Albino and Pigmented Mice |
| title_full | Assessment of Choroidal Vasculature and Innate Immune Cells in the Eyes of Albino and Pigmented Mice |
| title_fullStr | Assessment of Choroidal Vasculature and Innate Immune Cells in the Eyes of Albino and Pigmented Mice |
| title_full_unstemmed | Assessment of Choroidal Vasculature and Innate Immune Cells in the Eyes of Albino and Pigmented Mice |
| title_short | Assessment of Choroidal Vasculature and Innate Immune Cells in the Eyes of Albino and Pigmented Mice |
| title_sort | assessment of choroidal vasculature and innate immune cells in the eyes of albino and pigmented mice |
| topic | choriocapillaris mast cells macrophages smooth muscle cells uveal diseases age-related macular degeneration |
| url | https://www.mdpi.com/2073-4409/11/20/3329 |
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