Simultaneous RP-HPLC and UV Spectroscopic Method Development and Validation for Estimation of Ibandronate Sodium in Bulk and Pharmaceutical Dosage Form
The present study describes a simple, accurate, precise and cost effective UV-Vis Spectroscopic and RP-HPLC method for the estimation of Ibandronate sodium (IBN). The determination of Ibandronate sodium (IBN) was performed by both UV and RP-HPLC method using 215 nm as the determination wavelength. T...
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| Format: | Article |
| Language: | English |
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Iranian Chemical Society
2015-12-01
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| Series: | Analytical and Bioanalytical Chemistry Research |
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| Online Access: | http://www.analchemres.org/article_10758_6e7865b53685ad91f446371261f18ae7.pdf |
| _version_ | 1831756291623616512 |
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| author | Priyanka Bose Suddhasattya Dey Souvik Basak Shreya Shah Anjan De |
| author_facet | Priyanka Bose Suddhasattya Dey Souvik Basak Shreya Shah Anjan De |
| author_sort | Priyanka Bose |
| collection | DOAJ |
| description | The present study describes a simple, accurate, precise and cost effective UV-Vis Spectroscopic and RP-HPLC method for the estimation of Ibandronate sodium (IBN). The determination of Ibandronate sodium (IBN) was performed by both UV and RP-HPLC method using 215 nm as the determination wavelength. The drug was dissolved in NaOH solution (0.1N NaOH) for estimation in UV and in distilled water for the estimation in RP-HPLC using mobile phase 0.01 M Sodium dihydrogen phosphate (NaH2PO4): Acetonitrile (80:20), pH being adjusted to 3.3 with 10% ortho-phosphoric acid. A linear response was observed in the range of 10-50 μg ml-1 (R2 = 0.9981) for UV-Spectroscopy, whereas for RP-HPLC the linear response was observed in the range of 20-70 μg ml-1 (R2 = 0.9965). The limits of quantitation (LOQ) were estimated as 0.1 μg ml-1 and 0.05 μg ml-1, respectively for UV and RP-HPLC respectively. The recoveries of IBN from the marketed formulation were found to be within 100 ± 2% by both the methods. These methods were then effectively applied for the estimation of Boniva (tablet) and the results were obtained according to nominal content. The statistical analysis revealed that there is no significant difference (p > 0.05) between UV and HPLC methods regarding validation parameters and assay content. |
| first_indexed | 2024-12-22T00:10:08Z |
| format | Article |
| id | doaj.art-a3d3304abc9b439bb6c69a30d156305b |
| institution | Directory Open Access Journal |
| issn | 2383-093X 2383-093X |
| language | English |
| last_indexed | 2024-12-22T00:10:08Z |
| publishDate | 2015-12-01 |
| publisher | Iranian Chemical Society |
| record_format | Article |
| series | Analytical and Bioanalytical Chemistry Research |
| spelling | doaj.art-a3d3304abc9b439bb6c69a30d156305b2022-12-21T18:45:27ZengIranian Chemical SocietyAnalytical and Bioanalytical Chemistry Research2383-093X2383-093X2015-12-01229911210.22036/abcr.2015.1075810758Simultaneous RP-HPLC and UV Spectroscopic Method Development and Validation for Estimation of Ibandronate Sodium in Bulk and Pharmaceutical Dosage FormPriyanka Bose0Suddhasattya Dey1Souvik Basak2Shreya Shah3Anjan De4Dr. B.C. Roy College of Pharmacy & AHSWBUTWBUTSigma Institute of PharmacyWBUTThe present study describes a simple, accurate, precise and cost effective UV-Vis Spectroscopic and RP-HPLC method for the estimation of Ibandronate sodium (IBN). The determination of Ibandronate sodium (IBN) was performed by both UV and RP-HPLC method using 215 nm as the determination wavelength. The drug was dissolved in NaOH solution (0.1N NaOH) for estimation in UV and in distilled water for the estimation in RP-HPLC using mobile phase 0.01 M Sodium dihydrogen phosphate (NaH2PO4): Acetonitrile (80:20), pH being adjusted to 3.3 with 10% ortho-phosphoric acid. A linear response was observed in the range of 10-50 μg ml-1 (R2 = 0.9981) for UV-Spectroscopy, whereas for RP-HPLC the linear response was observed in the range of 20-70 μg ml-1 (R2 = 0.9965). The limits of quantitation (LOQ) were estimated as 0.1 μg ml-1 and 0.05 μg ml-1, respectively for UV and RP-HPLC respectively. The recoveries of IBN from the marketed formulation were found to be within 100 ± 2% by both the methods. These methods were then effectively applied for the estimation of Boniva (tablet) and the results were obtained according to nominal content. The statistical analysis revealed that there is no significant difference (p > 0.05) between UV and HPLC methods regarding validation parameters and assay content.http://www.analchemres.org/article_10758_6e7865b53685ad91f446371261f18ae7.pdfIbandronate sodium (IBN)UV-vis spectroscopyRP-HPLCCalibration curveLOD |
| spellingShingle | Priyanka Bose Suddhasattya Dey Souvik Basak Shreya Shah Anjan De Simultaneous RP-HPLC and UV Spectroscopic Method Development and Validation for Estimation of Ibandronate Sodium in Bulk and Pharmaceutical Dosage Form Analytical and Bioanalytical Chemistry Research Ibandronate sodium (IBN) UV-vis spectroscopy RP-HPLC Calibration curve LOD |
| title | Simultaneous RP-HPLC and UV Spectroscopic Method Development and Validation for Estimation of Ibandronate Sodium in Bulk and Pharmaceutical Dosage Form |
| title_full | Simultaneous RP-HPLC and UV Spectroscopic Method Development and Validation for Estimation of Ibandronate Sodium in Bulk and Pharmaceutical Dosage Form |
| title_fullStr | Simultaneous RP-HPLC and UV Spectroscopic Method Development and Validation for Estimation of Ibandronate Sodium in Bulk and Pharmaceutical Dosage Form |
| title_full_unstemmed | Simultaneous RP-HPLC and UV Spectroscopic Method Development and Validation for Estimation of Ibandronate Sodium in Bulk and Pharmaceutical Dosage Form |
| title_short | Simultaneous RP-HPLC and UV Spectroscopic Method Development and Validation for Estimation of Ibandronate Sodium in Bulk and Pharmaceutical Dosage Form |
| title_sort | simultaneous rp hplc and uv spectroscopic method development and validation for estimation of ibandronate sodium in bulk and pharmaceutical dosage form |
| topic | Ibandronate sodium (IBN) UV-vis spectroscopy RP-HPLC Calibration curve LOD |
| url | http://www.analchemres.org/article_10758_6e7865b53685ad91f446371261f18ae7.pdf |
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