Gene diversity explains variation in biological features of insect killing fungus, Beauveria bassiana
Abstract Beauveria bassiana is a species complex whose isolates show considerable natural genetic variability. However, little is known about how this genetic diversity affects the fungus performance. Herein, we characterized the diversity of genes involved in various mechanisms of the infective cyc...
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2021-01-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-020-78910-1 |
| _version_ | 1818858485290369024 |
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| author | Laila Gasmi Sehyeon Baek Jong Cheol Kim Sihyeon Kim Mi Rong Lee So Eun Park Tae Young Shin Se Jin Lee Bruce L. Parker Jae Su Kim |
| author_facet | Laila Gasmi Sehyeon Baek Jong Cheol Kim Sihyeon Kim Mi Rong Lee So Eun Park Tae Young Shin Se Jin Lee Bruce L. Parker Jae Su Kim |
| author_sort | Laila Gasmi |
| collection | DOAJ |
| description | Abstract Beauveria bassiana is a species complex whose isolates show considerable natural genetic variability. However, little is known about how this genetic diversity affects the fungus performance. Herein, we characterized the diversity of genes involved in various mechanisms of the infective cycle of 42 isolates that have different growth rates, thermotolerance and virulence. The analysed genes showed general genetic diversity measured as non-synonymous changes (NSC) and copy number variation (CNV), with most of them being subjected to positive episodic diversifying selection. Correlation analyses between NSC or CNV and the isolate virulence, thermotolerance and growth rate revealed that various genes shaped the biological features of the fungus. Lectin-like, mucin signalling, Biotrophy associated and chitinase genes NSCs correlated with the three biological features of B. bassiana. In addition, other genes (i.e. DNA photolyase and cyclophilin B) that had relatively conserved sequences, had variable CNs across the isolates which were correlated with the variability of either virulence or thermotolerance of B. bassiana isolates. The data obtained is important for a better understanding of population structure, ecological and potential impact when isolates are used as mycoinsecticides and can justify industrialization of new isolates. |
| first_indexed | 2024-12-19T08:57:02Z |
| format | Article |
| id | doaj.art-a3d4de234d1d47df91e408dfe3d97551 |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-12-19T08:57:02Z |
| publishDate | 2021-01-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-a3d4de234d1d47df91e408dfe3d975512022-12-21T20:28:36ZengNature PortfolioScientific Reports2045-23222021-01-0111111210.1038/s41598-020-78910-1Gene diversity explains variation in biological features of insect killing fungus, Beauveria bassianaLaila Gasmi0Sehyeon Baek1Jong Cheol Kim2Sihyeon Kim3Mi Rong Lee4So Eun Park5Tae Young Shin6Se Jin Lee7Bruce L. Parker8Jae Su Kim9Department of Agricultural Biology, Jeonbuk National UniversityDepartment of Agricultural Biology, Jeonbuk National UniversityDepartment of Agricultural Biology, Jeonbuk National UniversityDepartment of Agricultural Biology, Jeonbuk National UniversityDepartment of Agricultural Biology, Jeonbuk National UniversityDepartment of Agricultural Biology, Jeonbuk National UniversityDepartment of Agricultural Biology, Jeonbuk National UniversityDepartment of Microbiology and Cell Science, University of FloridaEntomology Research Laboratory, University of VermontDepartment of Agricultural Biology, Jeonbuk National UniversityAbstract Beauveria bassiana is a species complex whose isolates show considerable natural genetic variability. However, little is known about how this genetic diversity affects the fungus performance. Herein, we characterized the diversity of genes involved in various mechanisms of the infective cycle of 42 isolates that have different growth rates, thermotolerance and virulence. The analysed genes showed general genetic diversity measured as non-synonymous changes (NSC) and copy number variation (CNV), with most of them being subjected to positive episodic diversifying selection. Correlation analyses between NSC or CNV and the isolate virulence, thermotolerance and growth rate revealed that various genes shaped the biological features of the fungus. Lectin-like, mucin signalling, Biotrophy associated and chitinase genes NSCs correlated with the three biological features of B. bassiana. In addition, other genes (i.e. DNA photolyase and cyclophilin B) that had relatively conserved sequences, had variable CNs across the isolates which were correlated with the variability of either virulence or thermotolerance of B. bassiana isolates. The data obtained is important for a better understanding of population structure, ecological and potential impact when isolates are used as mycoinsecticides and can justify industrialization of new isolates.https://doi.org/10.1038/s41598-020-78910-1 |
| spellingShingle | Laila Gasmi Sehyeon Baek Jong Cheol Kim Sihyeon Kim Mi Rong Lee So Eun Park Tae Young Shin Se Jin Lee Bruce L. Parker Jae Su Kim Gene diversity explains variation in biological features of insect killing fungus, Beauveria bassiana Scientific Reports |
| title | Gene diversity explains variation in biological features of insect killing fungus, Beauveria bassiana |
| title_full | Gene diversity explains variation in biological features of insect killing fungus, Beauveria bassiana |
| title_fullStr | Gene diversity explains variation in biological features of insect killing fungus, Beauveria bassiana |
| title_full_unstemmed | Gene diversity explains variation in biological features of insect killing fungus, Beauveria bassiana |
| title_short | Gene diversity explains variation in biological features of insect killing fungus, Beauveria bassiana |
| title_sort | gene diversity explains variation in biological features of insect killing fungus beauveria bassiana |
| url | https://doi.org/10.1038/s41598-020-78910-1 |
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