Antibiotic resistance pattern of uropathogenic Escherichia coli isolated from children with symptomatic urinary tract infection in Moscow, Russia
Background and Aim: Uropathogenic Escherichia coli (UPEC) is commonly involved in urinary tract infections (UTIs), which are generally treated with antibiotics. However, the emergence of multidrug-resistant (MDR) strains of UPEC has made the treatment difficult. There is thus a need to continuously...
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| Format: | Article |
| Language: | English |
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Veterinary World
2021-11-01
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| Series: | International Journal of One Health |
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| Online Access: | https://www.onehealthjournal.org/Vol.7/No.2/9.pdf |
| _version_ | 1830273662343708672 |
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| author | Souadkia Sarra Mbarga Manga Joseph Arsene Volina Elena Grigorievna Podoprigora Irina Victorovna Yashina Natalia Vyacheslavovna Borechova Marina Nikolaïevna |
| author_facet | Souadkia Sarra Mbarga Manga Joseph Arsene Volina Elena Grigorievna Podoprigora Irina Victorovna Yashina Natalia Vyacheslavovna Borechova Marina Nikolaïevna |
| author_sort | Souadkia Sarra |
| collection | DOAJ |
| description | Background and Aim: Uropathogenic Escherichia coli (UPEC) is commonly involved in urinary tract infections (UTIs), which are generally treated with antibiotics. However, the emergence of multidrug-resistant (MDR) strains of UPEC has made the treatment difficult. There is thus a need to continuously assess their sensitivity to antibiotics. This study aimed to determine the antibiotic resistance patterns and MDR phenotypes of UPEC strains isolated from children diagnosed with UTIs at the Russian Children's Clinical Hospital in Moscow, Russia.
Materials and Methods: Kirby–Bauer's disc diffusion method was used to study the sensitivity to antibiotics of 106 UPEC isolates from urine specimens from children (aged from 9 months to 18 years old) diagnosed with UTIs. The results were interpreted in accordance with the Clinical and Laboratory Standards Institute guidelines and the correlations of variables with the degree to which each antibiotic inhibited the UPEC strains in terms of diameter on the disc were determined using Spearman's rank correlation test. A t-test and principal component analysis were performed to visualize the correlations of the susceptibility of UPEC to antibiotics with the age and sex of the patients. Statistical significance was set at p≤0.05.
Results: Among the 106 UPEC strains tested, none (0%) showed resistance to fosfomycin (FO), while 84 (79.2%) were resistant (R) to at least one antibiotic. The highest rates of resistance were observed to amoxicillin (69.8%), ampicillin (62.3%), cefazolin (39.6%), trimethoprim (TR) (37.7%), ceftriaxone (34.9%), and tetracycline (33.0%). Interestingly, 22 (20.8%) strains were R to imipenem. UPEC isolates from males aged 1-6 years were more R to antibiotics than those from the other groups, with the exception of TR, to which UPEC isolates from females aged 13-18 years old were less sensitive (S). The multidrug-resistance (MDR) index ranged between 0.00 and 0.75 and we found that more than a quarter of UPEC (31/106) had an MDR index ≥0.5 and only 22 (20.7%) strains were S to all antibiotics tested (MDR index=0). Finally, Spearman's rank correlation test showed that, with the exception of FO, there were correlations between the inhibition diameters of all other antibiotics.
Conclusion: FO is the only antibiotic to which all UPECs were S and may be suggested as the first line of treatment for UPEC. Further research is needed to continue monitoring antibiotic resistance and to investigate the genetic features associated with such resistance observed in this study. |
| first_indexed | 2024-12-18T23:49:12Z |
| format | Article |
| id | doaj.art-a3d61f7f513041848a30d9332cb930cf |
| institution | Directory Open Access Journal |
| issn | 2455-5673 2455-8931 |
| language | English |
| last_indexed | 2024-12-18T23:49:12Z |
| publishDate | 2021-11-01 |
| publisher | Veterinary World |
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| series | International Journal of One Health |
| spelling | doaj.art-a3d61f7f513041848a30d9332cb930cf2022-12-21T20:47:05ZengVeterinary WorldInternational Journal of One Health2455-56732455-89312021-11-017221221910.14202/IJOH.2021.212-219Antibiotic resistance pattern of uropathogenic Escherichia coli isolated from children with symptomatic urinary tract infection in Moscow, RussiaSouadkia Sarra0https://orcid.org/0000-0001-6761-0848Mbarga Manga Joseph Arsene1https://orcid.org/0000-0001-9626-9247Volina Elena Grigorievna2https://orcid.org/0000-0002-0013-7627Podoprigora Irina Victorovna3https://orcid.org/0000-0003-4099-2967Yashina Natalia Vyacheslavovna4https://orcid.org/0000-0003-1372-5257Borechova Marina Nikolaïevna5https://orcid.org/0000-0001-8605-0970Department of Microbiology and Virology, Institute of Medicine, RUDN University, Moscow, Russia.Department of Microbiology and Virology, Institute of Medicine, RUDN University, Moscow, Russia.Department of Microbiology and Virology, Institute of Medicine, RUDN University, Moscow, Russia.Department of Microbiology and Virology, Institute of Medicine, RUDN University, Moscow, Russia.Department of Microbiology and Virology, Institute of Medicine, RUDN University, Moscow, Russia.Department of Microbiology and Virology, Institute of Medicine, RUDN University, Moscow, Russia.Background and Aim: Uropathogenic Escherichia coli (UPEC) is commonly involved in urinary tract infections (UTIs), which are generally treated with antibiotics. However, the emergence of multidrug-resistant (MDR) strains of UPEC has made the treatment difficult. There is thus a need to continuously assess their sensitivity to antibiotics. This study aimed to determine the antibiotic resistance patterns and MDR phenotypes of UPEC strains isolated from children diagnosed with UTIs at the Russian Children's Clinical Hospital in Moscow, Russia. Materials and Methods: Kirby–Bauer's disc diffusion method was used to study the sensitivity to antibiotics of 106 UPEC isolates from urine specimens from children (aged from 9 months to 18 years old) diagnosed with UTIs. The results were interpreted in accordance with the Clinical and Laboratory Standards Institute guidelines and the correlations of variables with the degree to which each antibiotic inhibited the UPEC strains in terms of diameter on the disc were determined using Spearman's rank correlation test. A t-test and principal component analysis were performed to visualize the correlations of the susceptibility of UPEC to antibiotics with the age and sex of the patients. Statistical significance was set at p≤0.05. Results: Among the 106 UPEC strains tested, none (0%) showed resistance to fosfomycin (FO), while 84 (79.2%) were resistant (R) to at least one antibiotic. The highest rates of resistance were observed to amoxicillin (69.8%), ampicillin (62.3%), cefazolin (39.6%), trimethoprim (TR) (37.7%), ceftriaxone (34.9%), and tetracycline (33.0%). Interestingly, 22 (20.8%) strains were R to imipenem. UPEC isolates from males aged 1-6 years were more R to antibiotics than those from the other groups, with the exception of TR, to which UPEC isolates from females aged 13-18 years old were less sensitive (S). The multidrug-resistance (MDR) index ranged between 0.00 and 0.75 and we found that more than a quarter of UPEC (31/106) had an MDR index ≥0.5 and only 22 (20.7%) strains were S to all antibiotics tested (MDR index=0). Finally, Spearman's rank correlation test showed that, with the exception of FO, there were correlations between the inhibition diameters of all other antibiotics. Conclusion: FO is the only antibiotic to which all UPECs were S and may be suggested as the first line of treatment for UPEC. Further research is needed to continue monitoring antibiotic resistance and to investigate the genetic features associated with such resistance observed in this study.https://www.onehealthjournal.org/Vol.7/No.2/9.pdfantibioticsmultidrug resistanceurinary tract infectionsuropathogenic escherichia coli |
| spellingShingle | Souadkia Sarra Mbarga Manga Joseph Arsene Volina Elena Grigorievna Podoprigora Irina Victorovna Yashina Natalia Vyacheslavovna Borechova Marina Nikolaïevna Antibiotic resistance pattern of uropathogenic Escherichia coli isolated from children with symptomatic urinary tract infection in Moscow, Russia International Journal of One Health antibiotics multidrug resistance urinary tract infections uropathogenic escherichia coli |
| title | Antibiotic resistance pattern of uropathogenic Escherichia coli isolated from children with symptomatic urinary tract infection in Moscow, Russia |
| title_full | Antibiotic resistance pattern of uropathogenic Escherichia coli isolated from children with symptomatic urinary tract infection in Moscow, Russia |
| title_fullStr | Antibiotic resistance pattern of uropathogenic Escherichia coli isolated from children with symptomatic urinary tract infection in Moscow, Russia |
| title_full_unstemmed | Antibiotic resistance pattern of uropathogenic Escherichia coli isolated from children with symptomatic urinary tract infection in Moscow, Russia |
| title_short | Antibiotic resistance pattern of uropathogenic Escherichia coli isolated from children with symptomatic urinary tract infection in Moscow, Russia |
| title_sort | antibiotic resistance pattern of uropathogenic escherichia coli isolated from children with symptomatic urinary tract infection in moscow russia |
| topic | antibiotics multidrug resistance urinary tract infections uropathogenic escherichia coli |
| url | https://www.onehealthjournal.org/Vol.7/No.2/9.pdf |
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