A rapid influenza diagnostic test based on detection of viral neuraminidase activity
Abstract Current methods used for diagnosis of acute infection of pathogens rely on detection of nucleic acids, antigens, or certain classes of antibodies such as IgM. Here we report a virus enzyme assay as an alternative to these methods for detection of acute viral infection. In this method, we us...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2022-01-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-021-04538-4 |
| _version_ | 1818752912974675968 |
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| author | Xuexiang Lin Xiao-Yu Liu Bo Zhang Ai-Qing Qin Kwok-Min Hui Kevin Shi Yang Liu Don Gabriel X. James Li |
| author_facet | Xuexiang Lin Xiao-Yu Liu Bo Zhang Ai-Qing Qin Kwok-Min Hui Kevin Shi Yang Liu Don Gabriel X. James Li |
| author_sort | Xuexiang Lin |
| collection | DOAJ |
| description | Abstract Current methods used for diagnosis of acute infection of pathogens rely on detection of nucleic acids, antigens, or certain classes of antibodies such as IgM. Here we report a virus enzyme assay as an alternative to these methods for detection of acute viral infection. In this method, we used a luciferin derivative as the substrate for detection of the enzyme activity of influenza viral neuraminidase as a means for diagnosis of influenza. The resulting commercial test, the qFLU Dx Test, uses a different supply chain that does not compete with those for the current tests. The assay reagents were formulated as a master mix that accommodated both the neuraminidase and luciferase reactions, thereby enabling rapid and prolonged production of stable light signal in the presence of influenza virus in the sample. The assay was evaluated using depository throat swab specimens. As expected, the assay exhibited similar detection rates for all influenza types and subtypes except for A(H7N9), which exhibited lower detection rate due to lower viral titer in the specimens. When throat swab specimens were diluted with the sample buffer of the test kit and tested with the qFLU Dx Test. The sensitivity and specificity were 82.41% (95% confidence interval: 79.66–85.84%) and 95.39% (95% confidence interval: 94.32–96.46%), respectively, for these diluted specimens in comparison to a real-time polymerase chain reaction assay. The uniqueness of the qFLU Dx Test as an enzymatic assay makes it highly complementary with currently available methods. |
| first_indexed | 2024-12-18T04:59:00Z |
| format | Article |
| id | doaj.art-a3d76fe4febf4f79bb51a5355b44b37c |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-12-18T04:59:00Z |
| publishDate | 2022-01-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-a3d76fe4febf4f79bb51a5355b44b37c2022-12-21T21:20:11ZengNature PortfolioScientific Reports2045-23222022-01-011211810.1038/s41598-021-04538-4A rapid influenza diagnostic test based on detection of viral neuraminidase activityXuexiang Lin0Xiao-Yu Liu1Bo Zhang2Ai-Qing Qin3Kwok-Min Hui4Kevin Shi5Yang Liu6Don Gabriel7X. James Li8Shenzhen Institutes of Advanced Technology, Chinese Academy of SciencesCellex IncorporatedCellex IncorporatedCellex IncorporatedCellex IncorporatedCellex IncorporatedCellex IncorporatedCellex IncorporatedCellex IncorporatedAbstract Current methods used for diagnosis of acute infection of pathogens rely on detection of nucleic acids, antigens, or certain classes of antibodies such as IgM. Here we report a virus enzyme assay as an alternative to these methods for detection of acute viral infection. In this method, we used a luciferin derivative as the substrate for detection of the enzyme activity of influenza viral neuraminidase as a means for diagnosis of influenza. The resulting commercial test, the qFLU Dx Test, uses a different supply chain that does not compete with those for the current tests. The assay reagents were formulated as a master mix that accommodated both the neuraminidase and luciferase reactions, thereby enabling rapid and prolonged production of stable light signal in the presence of influenza virus in the sample. The assay was evaluated using depository throat swab specimens. As expected, the assay exhibited similar detection rates for all influenza types and subtypes except for A(H7N9), which exhibited lower detection rate due to lower viral titer in the specimens. When throat swab specimens were diluted with the sample buffer of the test kit and tested with the qFLU Dx Test. The sensitivity and specificity were 82.41% (95% confidence interval: 79.66–85.84%) and 95.39% (95% confidence interval: 94.32–96.46%), respectively, for these diluted specimens in comparison to a real-time polymerase chain reaction assay. The uniqueness of the qFLU Dx Test as an enzymatic assay makes it highly complementary with currently available methods.https://doi.org/10.1038/s41598-021-04538-4 |
| spellingShingle | Xuexiang Lin Xiao-Yu Liu Bo Zhang Ai-Qing Qin Kwok-Min Hui Kevin Shi Yang Liu Don Gabriel X. James Li A rapid influenza diagnostic test based on detection of viral neuraminidase activity Scientific Reports |
| title | A rapid influenza diagnostic test based on detection of viral neuraminidase activity |
| title_full | A rapid influenza diagnostic test based on detection of viral neuraminidase activity |
| title_fullStr | A rapid influenza diagnostic test based on detection of viral neuraminidase activity |
| title_full_unstemmed | A rapid influenza diagnostic test based on detection of viral neuraminidase activity |
| title_short | A rapid influenza diagnostic test based on detection of viral neuraminidase activity |
| title_sort | rapid influenza diagnostic test based on detection of viral neuraminidase activity |
| url | https://doi.org/10.1038/s41598-021-04538-4 |
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