In Vitro and In Vivo Study of Titanium Grade IV and Titanium Grade V Implants with Different Surface Treatments
The aim of our study is to evaluate different implant surface treatments using TiIV and TiV in in vitro and in vivo studies. An in vitro study was established comprising four study groups with treated and untreated TiIV titanium discs (TiIVT and TiIVNT) and treated and untreated TiV titanium discs (...
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MDPI AG
2020-03-01
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| Series: | Metals |
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| Online Access: | https://www.mdpi.com/2075-4701/10/4/449 |
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| author | Rosa-Maria Diaz-Sanchez Alvaro de-Paz-Carrion Maria-Angeles Serrera-Figallo Daniel Torres-Lagares Angel Barranco Juan-Rey León-Ramos Jose-Luis Gutierrez-Perez |
| author_facet | Rosa-Maria Diaz-Sanchez Alvaro de-Paz-Carrion Maria-Angeles Serrera-Figallo Daniel Torres-Lagares Angel Barranco Juan-Rey León-Ramos Jose-Luis Gutierrez-Perez |
| author_sort | Rosa-Maria Diaz-Sanchez |
| collection | DOAJ |
| description | The aim of our study is to evaluate different implant surface treatments using TiIV and TiV in in vitro and in vivo studies. An in vitro study was established comprising four study groups with treated and untreated TiIV titanium discs (TiIVT and TiIVNT) and treated and untreated TiV titanium discs (TiVT and TiVNT). The surface treatment consisted in a grit blasting treatment with alumina and double acid passivation to modify surface roughness. The surface chemical composition and the surface microstructure of the samples were analyzed. The titanium discs were subjected to cell cultures to determine cell adhesion and proliferation of osteoblasts on them. The in vivo study was carried out on the tibia of three New Zealand rabbits in which 18 implants divided into three experimental groups were placed (TiIVT, TiIVNT, and TiVT). Micro-computed tomography (micro-CT) was performed to determine bone density around the implants. The results showed that cell culture had minor adhesion and cell proliferation in TiIVT and TiVT within the first 6 and 24 h. However, no differences were found after 48 h. No statistically significant differences were found in the in vivo micro-CT and histological study; however, there was a positive trend in bone formation in the groups with a treated surface. Conclusions: All groups showed a similar response to in vitro cell proliferation cultures after 48 h. No statistically significant differences were found in the in vivo micro-CT and histological study. |
| first_indexed | 2024-03-11T10:11:05Z |
| format | Article |
| id | doaj.art-a3d84e54be764465a7441e8bf9b92bf8 |
| institution | Directory Open Access Journal |
| issn | 2075-4701 |
| language | English |
| last_indexed | 2024-03-11T10:11:05Z |
| publishDate | 2020-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Metals |
| spelling | doaj.art-a3d84e54be764465a7441e8bf9b92bf82023-11-16T14:33:07ZengMDPI AGMetals2075-47012020-03-0110444910.3390/met10040449In Vitro and In Vivo Study of Titanium Grade IV and Titanium Grade V Implants with Different Surface TreatmentsRosa-Maria Diaz-Sanchez0Alvaro de-Paz-Carrion1Maria-Angeles Serrera-Figallo2Daniel Torres-Lagares3Angel Barranco4Juan-Rey León-Ramos5Jose-Luis Gutierrez-Perez6Dental School, University of Seville, 41009 Seville, SpainDental School, University of Seville, 41009 Seville, SpainDental School, University of Seville, 41009 Seville, SpainDental School, University of Seville, 41009 Seville, SpainInstituto de Ciencia de Materiales de Sevilla, Consejo Superior de Investigaciones Científicas (CSIC-US), 41092 Seville, SpainInstituto de Ciencia de Materiales de Sevilla, Consejo Superior de Investigaciones Científicas (CSIC-US), 41092 Seville, SpainDental School, University of Seville, 41009 Seville, SpainThe aim of our study is to evaluate different implant surface treatments using TiIV and TiV in in vitro and in vivo studies. An in vitro study was established comprising four study groups with treated and untreated TiIV titanium discs (TiIVT and TiIVNT) and treated and untreated TiV titanium discs (TiVT and TiVNT). The surface treatment consisted in a grit blasting treatment with alumina and double acid passivation to modify surface roughness. The surface chemical composition and the surface microstructure of the samples were analyzed. The titanium discs were subjected to cell cultures to determine cell adhesion and proliferation of osteoblasts on them. The in vivo study was carried out on the tibia of three New Zealand rabbits in which 18 implants divided into three experimental groups were placed (TiIVT, TiIVNT, and TiVT). Micro-computed tomography (micro-CT) was performed to determine bone density around the implants. The results showed that cell culture had minor adhesion and cell proliferation in TiIVT and TiVT within the first 6 and 24 h. However, no differences were found after 48 h. No statistically significant differences were found in the in vivo micro-CT and histological study; however, there was a positive trend in bone formation in the groups with a treated surface. Conclusions: All groups showed a similar response to in vitro cell proliferation cultures after 48 h. No statistically significant differences were found in the in vivo micro-CT and histological study.https://www.mdpi.com/2075-4701/10/4/449in vitro studyin vivo studytitaniumgrade IVgrade Vsurface treatment |
| spellingShingle | Rosa-Maria Diaz-Sanchez Alvaro de-Paz-Carrion Maria-Angeles Serrera-Figallo Daniel Torres-Lagares Angel Barranco Juan-Rey León-Ramos Jose-Luis Gutierrez-Perez In Vitro and In Vivo Study of Titanium Grade IV and Titanium Grade V Implants with Different Surface Treatments Metals in vitro study in vivo study titanium grade IV grade V surface treatment |
| title | In Vitro and In Vivo Study of Titanium Grade IV and Titanium Grade V Implants with Different Surface Treatments |
| title_full | In Vitro and In Vivo Study of Titanium Grade IV and Titanium Grade V Implants with Different Surface Treatments |
| title_fullStr | In Vitro and In Vivo Study of Titanium Grade IV and Titanium Grade V Implants with Different Surface Treatments |
| title_full_unstemmed | In Vitro and In Vivo Study of Titanium Grade IV and Titanium Grade V Implants with Different Surface Treatments |
| title_short | In Vitro and In Vivo Study of Titanium Grade IV and Titanium Grade V Implants with Different Surface Treatments |
| title_sort | in vitro and in vivo study of titanium grade iv and titanium grade v implants with different surface treatments |
| topic | in vitro study in vivo study titanium grade IV grade V surface treatment |
| url | https://www.mdpi.com/2075-4701/10/4/449 |
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