Cellulase and xylanase production from Candida easanensis using agricultural wastes as a substrate

The production of cellulase and xylanase from Candida easanensis strain JK-8 was investigated. Different fermentation conditions were standardized for the growth and enzyme activity, the optimum being 72–96 hrs growth at initial pH 4.0, and cultivation temperature at 35°C. Of the different carbon...

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Main Authors: Jantaporn Thongekkaew, Wanlee Patangtasa, Apichat Jansri
Format: Article
Language:English
Published: Prince of Songkla University 2014-12-01
Series:Songklanakarin Journal of Science and Technology (SJST)
Subjects:
Online Access:http://rdo.psu.ac.th/sjstweb/journal/36-6/36-6-2.pdf
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author Jantaporn Thongekkaew
Wanlee Patangtasa
Apichat Jansri
author_facet Jantaporn Thongekkaew
Wanlee Patangtasa
Apichat Jansri
author_sort Jantaporn Thongekkaew
collection DOAJ
description The production of cellulase and xylanase from Candida easanensis strain JK-8 was investigated. Different fermentation conditions were standardized for the growth and enzyme activity, the optimum being 72–96 hrs growth at initial pH 4.0, and cultivation temperature at 35°C. Of the different carbon sources on cellulase production, carboxymethyl cellulose gave the maximum production of 0.23 UmL-1. Among the carbon sources on xylanase production, the maximum enzyme activity was achieved in the medium containing Birchwood xylan (1.14 UmL-1). Amongst different agricultural waste samples (such as rice straw, corn husk, and sugarcane bagasse), corn husk gave the highest yields of cellulase and xylanase and the activities were 0.089 and 0.82 UmL-1, respectively. This study suggests that corn husk could be utilized as a carbon source for economical production of cellulase and xylanase by C. easanensis JK-8. This may in turn reduce the cost of enzyme production leading to efficient use of ligno-cellulosic materials to produce value-added products.
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spelling doaj.art-a3d89938ebdf49299ea85b164b91429b2022-12-22T03:32:24ZengPrince of Songkla UniversitySongklanakarin Journal of Science and Technology (SJST)0125-33952014-12-01366607613Cellulase and xylanase production from Candida easanensis using agricultural wastes as a substrateJantaporn Thongekkaew0Wanlee Patangtasa1Apichat Jansri2Department of Biological Science, Faculty of Science, Ubon Ratchathani University, Warin Chamrap, Ubon Ratchathani, 34190 Thailand.Department of Biological Science, Faculty of Science, Ubon Ratchathani University, Warin Chamrap, Ubon Ratchathani, 34190 Thailand.Department of Biological Science, Faculty of Science, Ubon Ratchathani University, Warin Chamrap, Ubon Ratchathani, 34190 Thailand.The production of cellulase and xylanase from Candida easanensis strain JK-8 was investigated. Different fermentation conditions were standardized for the growth and enzyme activity, the optimum being 72–96 hrs growth at initial pH 4.0, and cultivation temperature at 35°C. Of the different carbon sources on cellulase production, carboxymethyl cellulose gave the maximum production of 0.23 UmL-1. Among the carbon sources on xylanase production, the maximum enzyme activity was achieved in the medium containing Birchwood xylan (1.14 UmL-1). Amongst different agricultural waste samples (such as rice straw, corn husk, and sugarcane bagasse), corn husk gave the highest yields of cellulase and xylanase and the activities were 0.089 and 0.82 UmL-1, respectively. This study suggests that corn husk could be utilized as a carbon source for economical production of cellulase and xylanase by C. easanensis JK-8. This may in turn reduce the cost of enzyme production leading to efficient use of ligno-cellulosic materials to produce value-added products.http://rdo.psu.ac.th/sjstweb/journal/36-6/36-6-2.pdfcellulasexylanaseCandida easanensisagricultural waste
spellingShingle Jantaporn Thongekkaew
Wanlee Patangtasa
Apichat Jansri
Cellulase and xylanase production from Candida easanensis using agricultural wastes as a substrate
Songklanakarin Journal of Science and Technology (SJST)
cellulase
xylanase
Candida easanensis
agricultural waste
title Cellulase and xylanase production from Candida easanensis using agricultural wastes as a substrate
title_full Cellulase and xylanase production from Candida easanensis using agricultural wastes as a substrate
title_fullStr Cellulase and xylanase production from Candida easanensis using agricultural wastes as a substrate
title_full_unstemmed Cellulase and xylanase production from Candida easanensis using agricultural wastes as a substrate
title_short Cellulase and xylanase production from Candida easanensis using agricultural wastes as a substrate
title_sort cellulase and xylanase production from candida easanensis using agricultural wastes as a substrate
topic cellulase
xylanase
Candida easanensis
agricultural waste
url http://rdo.psu.ac.th/sjstweb/journal/36-6/36-6-2.pdf
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AT apichatjansri cellulaseandxylanaseproductionfromcandidaeasanensisusingagriculturalwastesasasubstrate