Cellulase and xylanase production from Candida easanensis using agricultural wastes as a substrate
The production of cellulase and xylanase from Candida easanensis strain JK-8 was investigated. Different fermentation conditions were standardized for the growth and enzyme activity, the optimum being 72–96 hrs growth at initial pH 4.0, and cultivation temperature at 35°C. Of the different carbon...
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Format: | Article |
Language: | English |
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Prince of Songkla University
2014-12-01
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Series: | Songklanakarin Journal of Science and Technology (SJST) |
Subjects: | |
Online Access: | http://rdo.psu.ac.th/sjstweb/journal/36-6/36-6-2.pdf |
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author | Jantaporn Thongekkaew Wanlee Patangtasa Apichat Jansri |
author_facet | Jantaporn Thongekkaew Wanlee Patangtasa Apichat Jansri |
author_sort | Jantaporn Thongekkaew |
collection | DOAJ |
description | The production of cellulase and xylanase from Candida easanensis strain JK-8 was investigated. Different fermentation
conditions were standardized for the growth and enzyme activity, the optimum being 72–96 hrs growth at initial pH 4.0,
and cultivation temperature at 35°C. Of the different carbon sources on cellulase production, carboxymethyl cellulose gave
the maximum production of 0.23 UmL-1. Among the carbon sources on xylanase production, the maximum enzyme activity
was achieved in the medium containing Birchwood xylan (1.14 UmL-1). Amongst different agricultural waste samples (such as
rice straw, corn husk, and sugarcane bagasse), corn husk gave the highest yields of cellulase and xylanase and the activities
were 0.089 and 0.82 UmL-1, respectively. This study suggests that corn husk could be utilized as a carbon source for economical
production of cellulase and xylanase by C. easanensis JK-8. This may in turn reduce the cost of enzyme production
leading to efficient use of ligno-cellulosic materials to produce value-added products. |
first_indexed | 2024-04-12T12:52:45Z |
format | Article |
id | doaj.art-a3d89938ebdf49299ea85b164b91429b |
institution | Directory Open Access Journal |
issn | 0125-3395 |
language | English |
last_indexed | 2024-04-12T12:52:45Z |
publishDate | 2014-12-01 |
publisher | Prince of Songkla University |
record_format | Article |
series | Songklanakarin Journal of Science and Technology (SJST) |
spelling | doaj.art-a3d89938ebdf49299ea85b164b91429b2022-12-22T03:32:24ZengPrince of Songkla UniversitySongklanakarin Journal of Science and Technology (SJST)0125-33952014-12-01366607613Cellulase and xylanase production from Candida easanensis using agricultural wastes as a substrateJantaporn Thongekkaew0Wanlee Patangtasa1Apichat Jansri2Department of Biological Science, Faculty of Science, Ubon Ratchathani University, Warin Chamrap, Ubon Ratchathani, 34190 Thailand.Department of Biological Science, Faculty of Science, Ubon Ratchathani University, Warin Chamrap, Ubon Ratchathani, 34190 Thailand.Department of Biological Science, Faculty of Science, Ubon Ratchathani University, Warin Chamrap, Ubon Ratchathani, 34190 Thailand.The production of cellulase and xylanase from Candida easanensis strain JK-8 was investigated. Different fermentation conditions were standardized for the growth and enzyme activity, the optimum being 72–96 hrs growth at initial pH 4.0, and cultivation temperature at 35°C. Of the different carbon sources on cellulase production, carboxymethyl cellulose gave the maximum production of 0.23 UmL-1. Among the carbon sources on xylanase production, the maximum enzyme activity was achieved in the medium containing Birchwood xylan (1.14 UmL-1). Amongst different agricultural waste samples (such as rice straw, corn husk, and sugarcane bagasse), corn husk gave the highest yields of cellulase and xylanase and the activities were 0.089 and 0.82 UmL-1, respectively. This study suggests that corn husk could be utilized as a carbon source for economical production of cellulase and xylanase by C. easanensis JK-8. This may in turn reduce the cost of enzyme production leading to efficient use of ligno-cellulosic materials to produce value-added products.http://rdo.psu.ac.th/sjstweb/journal/36-6/36-6-2.pdfcellulasexylanaseCandida easanensisagricultural waste |
spellingShingle | Jantaporn Thongekkaew Wanlee Patangtasa Apichat Jansri Cellulase and xylanase production from Candida easanensis using agricultural wastes as a substrate Songklanakarin Journal of Science and Technology (SJST) cellulase xylanase Candida easanensis agricultural waste |
title | Cellulase and xylanase production from Candida easanensis using agricultural wastes as a substrate |
title_full | Cellulase and xylanase production from Candida easanensis using agricultural wastes as a substrate |
title_fullStr | Cellulase and xylanase production from Candida easanensis using agricultural wastes as a substrate |
title_full_unstemmed | Cellulase and xylanase production from Candida easanensis using agricultural wastes as a substrate |
title_short | Cellulase and xylanase production from Candida easanensis using agricultural wastes as a substrate |
title_sort | cellulase and xylanase production from candida easanensis using agricultural wastes as a substrate |
topic | cellulase xylanase Candida easanensis agricultural waste |
url | http://rdo.psu.ac.th/sjstweb/journal/36-6/36-6-2.pdf |
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