Minimization of MEDA Biochip-Size in Droplet Routing
With the increasing demand for fast, accurate, and reliable biological sensor systems, miniaturized systems have been aimed at droplet-based sensor systems and have been promising. A micro-electrode dot array (MEDA) biochip, which is one kind of the miniaturized systems for biochemical protocols suc...
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2022-04-01
|
| Series: | Biosensors |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2079-6374/12/5/277 |
| _version_ | 1797501257740451840 |
|---|---|
| author | Chiharu Shiro Hiroki Nishikawa Xiangbo Kong Hiroyuki Tomiyama Shigeru Yamashita |
| author_facet | Chiharu Shiro Hiroki Nishikawa Xiangbo Kong Hiroyuki Tomiyama Shigeru Yamashita |
| author_sort | Chiharu Shiro |
| collection | DOAJ |
| description | With the increasing demand for fast, accurate, and reliable biological sensor systems, miniaturized systems have been aimed at droplet-based sensor systems and have been promising. A micro-electrode dot array (MEDA) biochip, which is one kind of the miniaturized systems for biochemical protocols such as dispensing, dilutions, mixing, and so on, has become widespread due to enabling dynamical control of the droplets in microfluidic manipulations. In MEDA biochips, the electrowetting-on-dielectric (EWOD) technique stands out since it can actuate droplets with nano/picoliter volumes. Microelectrode cells on MEDA actuate multiple droplets simultaneously to route locations for the purpose of the biochemical operations. Taking advantage of the feature, droplets are often routed in parallel to achieve high-throughput outcomes. Regarding parallel manipulation of multiple droplets, however, the droplets are known to be initially placed at a distant position to avoid undesirable mixing. The droplets thus result in traveling a long way for a manipulation, and the required biochip size for routing is also enlarged. This paper proposes a routing method for droplets to reduce the biochip size on a MEDA biochip with the allowance of splitting during routing operations. We mathematically derive the routing problem, and the experiments demonstrate that our proposal can significantly reduce the biochip size by 70.8% on average, compared to the state-of-the-art method. |
| first_indexed | 2024-03-10T03:15:47Z |
| format | Article |
| id | doaj.art-a3dd155f19644810b37096d435b2cc0e |
| institution | Directory Open Access Journal |
| issn | 2079-6374 |
| language | English |
| last_indexed | 2024-03-10T03:15:47Z |
| publishDate | 2022-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biosensors |
| spelling | doaj.art-a3dd155f19644810b37096d435b2cc0e2023-11-23T10:15:09ZengMDPI AGBiosensors2079-63742022-04-0112527710.3390/bios12050277Minimization of MEDA Biochip-Size in Droplet RoutingChiharu Shiro0Hiroki Nishikawa1Xiangbo Kong2Hiroyuki Tomiyama3Shigeru Yamashita4Graduate School of Science and Engineering, Ritsumeikan University, Kusatsu 525-8577, JapanGraduate School of Science and Engineering, Ritsumeikan University, Kusatsu 525-8577, JapanGraduate School of Science and Engineering, Ritsumeikan University, Kusatsu 525-8577, JapanGraduate School of Science and Engineering, Ritsumeikan University, Kusatsu 525-8577, JapanCollege of Information Science and Engineering, Ritsumeikan University, Kusatsu 525-8577, JapanWith the increasing demand for fast, accurate, and reliable biological sensor systems, miniaturized systems have been aimed at droplet-based sensor systems and have been promising. A micro-electrode dot array (MEDA) biochip, which is one kind of the miniaturized systems for biochemical protocols such as dispensing, dilutions, mixing, and so on, has become widespread due to enabling dynamical control of the droplets in microfluidic manipulations. In MEDA biochips, the electrowetting-on-dielectric (EWOD) technique stands out since it can actuate droplets with nano/picoliter volumes. Microelectrode cells on MEDA actuate multiple droplets simultaneously to route locations for the purpose of the biochemical operations. Taking advantage of the feature, droplets are often routed in parallel to achieve high-throughput outcomes. Regarding parallel manipulation of multiple droplets, however, the droplets are known to be initially placed at a distant position to avoid undesirable mixing. The droplets thus result in traveling a long way for a manipulation, and the required biochip size for routing is also enlarged. This paper proposes a routing method for droplets to reduce the biochip size on a MEDA biochip with the allowance of splitting during routing operations. We mathematically derive the routing problem, and the experiments demonstrate that our proposal can significantly reduce the biochip size by 70.8% on average, compared to the state-of-the-art method.https://www.mdpi.com/2079-6374/12/5/277digital microfluidicsMEDAdroplet routingmathematical programming problem |
| spellingShingle | Chiharu Shiro Hiroki Nishikawa Xiangbo Kong Hiroyuki Tomiyama Shigeru Yamashita Minimization of MEDA Biochip-Size in Droplet Routing Biosensors digital microfluidics MEDA droplet routing mathematical programming problem |
| title | Minimization of MEDA Biochip-Size in Droplet Routing |
| title_full | Minimization of MEDA Biochip-Size in Droplet Routing |
| title_fullStr | Minimization of MEDA Biochip-Size in Droplet Routing |
| title_full_unstemmed | Minimization of MEDA Biochip-Size in Droplet Routing |
| title_short | Minimization of MEDA Biochip-Size in Droplet Routing |
| title_sort | minimization of meda biochip size in droplet routing |
| topic | digital microfluidics MEDA droplet routing mathematical programming problem |
| url | https://www.mdpi.com/2079-6374/12/5/277 |
| work_keys_str_mv | AT chiharushiro minimizationofmedabiochipsizeindropletrouting AT hirokinishikawa minimizationofmedabiochipsizeindropletrouting AT xiangbokong minimizationofmedabiochipsizeindropletrouting AT hiroyukitomiyama minimizationofmedabiochipsizeindropletrouting AT shigeruyamashita minimizationofmedabiochipsizeindropletrouting |