Soybean Oil Regulates the Fatty Acid Synthesis II System of <i>Bacillus amyloliquefaciens</i> LFB112 by Activating Acetyl-CoA Levels
[Background] <i>Bacillus</i> LFB112 is a strain of <i>Bacillus amyloliquefaciens</i> screened in our laboratory. Previous studies found that it has a strong ability for fatty acid metabolism and can improve the lipid metabolism of broilers when used as feed additives. [Method...
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MDPI AG
2023-04-01
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| Series: | Microorganisms |
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| Online Access: | https://www.mdpi.com/2076-2607/11/5/1164 |
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| author | Qiang Cheng Zhongxuan Li Jing Zhang Henan Guo Marhaba Ahmat Junhao Cheng Zaheer Abbas Zhengchang Hua Junyong Wang Yucui Tong Tiantian Yang Dayong Si Rijun Zhang |
| author_facet | Qiang Cheng Zhongxuan Li Jing Zhang Henan Guo Marhaba Ahmat Junhao Cheng Zaheer Abbas Zhengchang Hua Junyong Wang Yucui Tong Tiantian Yang Dayong Si Rijun Zhang |
| author_sort | Qiang Cheng |
| collection | DOAJ |
| description | [Background] <i>Bacillus</i> LFB112 is a strain of <i>Bacillus amyloliquefaciens</i> screened in our laboratory. Previous studies found that it has a strong ability for fatty acid metabolism and can improve the lipid metabolism of broilers when used as feed additives. [Methods] This study aimed to confirm the fatty acid metabolism of <i>Bacillus</i> LFB112. Sterilized soybean oil (SSO) was added to the Beef Peptone Yeast (BPY) medium, and its effect on fatty acid content in the supernatant and bacteria, as well as expression levels of genes related to fatty acid metabolism, were studied. The control group was the original culture medium without oil. [Results] Acetic acid produced by the SSO group of <i>Bacillus</i> LFB112 decreased, but the content of unsaturated fatty acids increased. The 1.6% SSO group significantly increased the contents of pyruvate and acetyl-CoA in the pellets. Furthermore, the mRNA levels of enzymes involved in the type II fatty acid synthesis pathway of FabD, FabH, FabG, FabZ, FabI, and FabF were up-regulated. [Conclusions] Soybean oil increased the content of acetyl-CoA in <i>Bacillus</i> LFB112, activated its type II fatty acid synthesis pathway, and improved the fatty acid metabolism level of <i>Bacillus</i> LFB112. These intriguing results pave the way for further investigations into the intricate interplay between <i>Bacillus</i> LFB112 and fatty acid metabolism, with potential applications in animal nutrition and feed additive development. |
| first_indexed | 2024-03-11T03:28:38Z |
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| institution | Directory Open Access Journal |
| issn | 2076-2607 |
| language | English |
| last_indexed | 2024-03-11T03:28:38Z |
| publishDate | 2023-04-01 |
| publisher | MDPI AG |
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| series | Microorganisms |
| spelling | doaj.art-a3dd25e373c84fcca80d0fdda69928b42023-11-18T02:32:44ZengMDPI AGMicroorganisms2076-26072023-04-01115116410.3390/microorganisms11051164Soybean Oil Regulates the Fatty Acid Synthesis II System of <i>Bacillus amyloliquefaciens</i> LFB112 by Activating Acetyl-CoA LevelsQiang Cheng0Zhongxuan Li1Jing Zhang2Henan Guo3Marhaba Ahmat4Junhao Cheng5Zaheer Abbas6Zhengchang Hua7Junyong Wang8Yucui Tong9Tiantian Yang10Dayong Si11Rijun Zhang12State Key Laboratory of Animal Nutrition, Laboratory of Feed Biotechnology, College of Animal Science & Technology, China Agricultural University, Beijing 100193, ChinaState Key Laboratory of Animal Nutrition, Laboratory of Feed Biotechnology, College of Animal Science & Technology, China Agricultural University, Beijing 100193, ChinaState Key Laboratory of Animal Nutrition, Laboratory of Feed Biotechnology, College of Animal Science & Technology, China Agricultural University, Beijing 100193, ChinaState Key Laboratory of Animal Nutrition, Laboratory of Feed Biotechnology, College of Animal Science & Technology, China Agricultural University, Beijing 100193, ChinaState Key Laboratory of Animal Nutrition, Laboratory of Feed Biotechnology, College of Animal Science & Technology, China Agricultural University, Beijing 100193, ChinaState Key Laboratory of Animal Nutrition, Laboratory of Feed Biotechnology, College of Animal Science & Technology, China Agricultural University, Beijing 100193, ChinaState Key Laboratory of Animal Nutrition, Laboratory of Feed Biotechnology, College of Animal Science & Technology, China Agricultural University, Beijing 100193, ChinaState Key Laboratory of Animal Nutrition, Laboratory of Feed Biotechnology, College of Animal Science & Technology, China Agricultural University, Beijing 100193, ChinaState Key Laboratory of Animal Nutrition, Laboratory of Feed Biotechnology, College of Animal Science & Technology, China Agricultural University, Beijing 100193, ChinaState Key Laboratory of Animal Nutrition, Laboratory of Feed Biotechnology, College of Animal Science & Technology, China Agricultural University, Beijing 100193, ChinaState Key Laboratory of Animal Nutrition, Laboratory of Feed Biotechnology, College of Animal Science & Technology, China Agricultural University, Beijing 100193, ChinaState Key Laboratory of Animal Nutrition, Laboratory of Feed Biotechnology, College of Animal Science & Technology, China Agricultural University, Beijing 100193, ChinaState Key Laboratory of Animal Nutrition, Laboratory of Feed Biotechnology, College of Animal Science & Technology, China Agricultural University, Beijing 100193, China[Background] <i>Bacillus</i> LFB112 is a strain of <i>Bacillus amyloliquefaciens</i> screened in our laboratory. Previous studies found that it has a strong ability for fatty acid metabolism and can improve the lipid metabolism of broilers when used as feed additives. [Methods] This study aimed to confirm the fatty acid metabolism of <i>Bacillus</i> LFB112. Sterilized soybean oil (SSO) was added to the Beef Peptone Yeast (BPY) medium, and its effect on fatty acid content in the supernatant and bacteria, as well as expression levels of genes related to fatty acid metabolism, were studied. The control group was the original culture medium without oil. [Results] Acetic acid produced by the SSO group of <i>Bacillus</i> LFB112 decreased, but the content of unsaturated fatty acids increased. The 1.6% SSO group significantly increased the contents of pyruvate and acetyl-CoA in the pellets. Furthermore, the mRNA levels of enzymes involved in the type II fatty acid synthesis pathway of FabD, FabH, FabG, FabZ, FabI, and FabF were up-regulated. [Conclusions] Soybean oil increased the content of acetyl-CoA in <i>Bacillus</i> LFB112, activated its type II fatty acid synthesis pathway, and improved the fatty acid metabolism level of <i>Bacillus</i> LFB112. These intriguing results pave the way for further investigations into the intricate interplay between <i>Bacillus</i> LFB112 and fatty acid metabolism, with potential applications in animal nutrition and feed additive development.https://www.mdpi.com/2076-2607/11/5/1164<i>Bacillus</i> LFB112fatty acid metabolismtype II fatty acid synthesis pathwayunsaturated fatty acids |
| spellingShingle | Qiang Cheng Zhongxuan Li Jing Zhang Henan Guo Marhaba Ahmat Junhao Cheng Zaheer Abbas Zhengchang Hua Junyong Wang Yucui Tong Tiantian Yang Dayong Si Rijun Zhang Soybean Oil Regulates the Fatty Acid Synthesis II System of <i>Bacillus amyloliquefaciens</i> LFB112 by Activating Acetyl-CoA Levels Microorganisms <i>Bacillus</i> LFB112 fatty acid metabolism type II fatty acid synthesis pathway unsaturated fatty acids |
| title | Soybean Oil Regulates the Fatty Acid Synthesis II System of <i>Bacillus amyloliquefaciens</i> LFB112 by Activating Acetyl-CoA Levels |
| title_full | Soybean Oil Regulates the Fatty Acid Synthesis II System of <i>Bacillus amyloliquefaciens</i> LFB112 by Activating Acetyl-CoA Levels |
| title_fullStr | Soybean Oil Regulates the Fatty Acid Synthesis II System of <i>Bacillus amyloliquefaciens</i> LFB112 by Activating Acetyl-CoA Levels |
| title_full_unstemmed | Soybean Oil Regulates the Fatty Acid Synthesis II System of <i>Bacillus amyloliquefaciens</i> LFB112 by Activating Acetyl-CoA Levels |
| title_short | Soybean Oil Regulates the Fatty Acid Synthesis II System of <i>Bacillus amyloliquefaciens</i> LFB112 by Activating Acetyl-CoA Levels |
| title_sort | soybean oil regulates the fatty acid synthesis ii system of i bacillus amyloliquefaciens i lfb112 by activating acetyl coa levels |
| topic | <i>Bacillus</i> LFB112 fatty acid metabolism type II fatty acid synthesis pathway unsaturated fatty acids |
| url | https://www.mdpi.com/2076-2607/11/5/1164 |
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