In vitro synergy of antibiotic combinations against planktonic and biofilm Pseudomonas aeruginosa
Aim: The combination of different antimicrobial agents and subsequent synergetic effects may be beneficial in treatment of infections. The aim of the present study was to determine antibiotic susceptibility patterns of clinical isolates of and the effect of different antibiotic combinations agains...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | deu |
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German Medical Science GMS Publishing House
2017-10-01
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| Series: | GMS Hygiene and Infection Control |
| Subjects: | |
| Online Access: | http://www.egms.de/static/en/journals/dgkh/2017-12/dgkh000302.shtml |
| _version_ | 1828454385718394880 |
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| author | Ghorbani, Hossein Memar, Mohammad Yousef Sefidan, Fatemeh Yeganeh Yekani, Mina Ghotaslou, Reza |
| author_facet | Ghorbani, Hossein Memar, Mohammad Yousef Sefidan, Fatemeh Yeganeh Yekani, Mina Ghotaslou, Reza |
| author_sort | Ghorbani, Hossein |
| collection | DOAJ |
| description | Aim: The combination of different antimicrobial agents and subsequent synergetic effects may be beneficial in treatment of infections. The aim of the present study was to determine antibiotic susceptibility patterns of clinical isolates of and the effect of different antibiotic combinations against the multidrug-resistant (MDR), biofilm-producing bacterium . Methods: Thirty-six clinical isolates were evaluated. The disk diffusion method was performed to determine antibiotic susceptibility patterns according to the Clinical and Laboratory Standards Institute (CLSI) guidelines. The minimum inhibitory concentration of antimicrobial agents for the test organisms was determined by the broth microdilution method. To determine synergetic effects of the combinations of agents, the checkerboard assay and the fractional inhibitory concentration were used. The biofilm inhibitory concentration was determined to detect any inhibitory effect of antibiotics against the biofilm. Results: High levels of resistance were detected against most antibiotics, except colistin and polymyxin. According to the disk diffusion method, 58.3% of isolates were MDR. A synergetic effect between amikacin/ceftazidime, tobramycin/colistin and ceftazidime/colistin was found in 55.6%, 58.3% and 52.8% of isolates, respectively. A significant synergetic effect against biofilm-producing isolates was observed for the combination of tobramycin (0.5–1 µg/ml) and clarithromycin (256–512 µg/ml).Conclusion: Combinations of antibiotics have a different activity on the biofilm and planktonic forms of . Consequently, separate detection of antibacterial and antibiofilm effects of the antibiotic combinations may be useful in guiding the antibiotic therapy. |
| first_indexed | 2024-12-11T00:20:48Z |
| format | Article |
| id | doaj.art-a3e125efc3c646a89c810655e89cd609 |
| institution | Directory Open Access Journal |
| issn | 2196-5226 |
| language | deu |
| last_indexed | 2024-12-11T00:20:48Z |
| publishDate | 2017-10-01 |
| publisher | German Medical Science GMS Publishing House |
| record_format | Article |
| series | GMS Hygiene and Infection Control |
| spelling | doaj.art-a3e125efc3c646a89c810655e89cd6092022-12-22T01:27:46ZdeuGerman Medical Science GMS Publishing HouseGMS Hygiene and Infection Control2196-52262017-10-0112Doc1710.3205/dgkh000302In vitro synergy of antibiotic combinations against planktonic and biofilm Pseudomonas aeruginosaGhorbani, Hossein0Memar, Mohammad Yousef1Sefidan, Fatemeh Yeganeh2Yekani, Mina3Ghotaslou, Reza4Infectious and Tropical Diseases Research Center, Tabriz University of Medical Sciences, Tabriz, IranDepartment of Microbiology, School of Medicine, Tabriz University of Medical Sciences, Tabriz, IranDepartment of Microbiology, School of Medicine, Tabriz University of Medical Sciences, Tabriz, IranDepartment of Microbiology, School of Medicine, Tabriz University of Medical Sciences, Tabriz, IranDepartment of Microbiology, School of Medicine, Tabriz University of Medical Sciences, Tabriz, IranAim: The combination of different antimicrobial agents and subsequent synergetic effects may be beneficial in treatment of infections. The aim of the present study was to determine antibiotic susceptibility patterns of clinical isolates of and the effect of different antibiotic combinations against the multidrug-resistant (MDR), biofilm-producing bacterium . Methods: Thirty-six clinical isolates were evaluated. The disk diffusion method was performed to determine antibiotic susceptibility patterns according to the Clinical and Laboratory Standards Institute (CLSI) guidelines. The minimum inhibitory concentration of antimicrobial agents for the test organisms was determined by the broth microdilution method. To determine synergetic effects of the combinations of agents, the checkerboard assay and the fractional inhibitory concentration were used. The biofilm inhibitory concentration was determined to detect any inhibitory effect of antibiotics against the biofilm. Results: High levels of resistance were detected against most antibiotics, except colistin and polymyxin. According to the disk diffusion method, 58.3% of isolates were MDR. A synergetic effect between amikacin/ceftazidime, tobramycin/colistin and ceftazidime/colistin was found in 55.6%, 58.3% and 52.8% of isolates, respectively. A significant synergetic effect against biofilm-producing isolates was observed for the combination of tobramycin (0.5–1 µg/ml) and clarithromycin (256–512 µg/ml).Conclusion: Combinations of antibiotics have a different activity on the biofilm and planktonic forms of . Consequently, separate detection of antibacterial and antibiofilm effects of the antibiotic combinations may be useful in guiding the antibiotic therapy.http://www.egms.de/static/en/journals/dgkh/2017-12/dgkh000302.shtmlantibiofilmcombination therapyPseudomonas aeruginosasynergetic effects |
| spellingShingle | Ghorbani, Hossein Memar, Mohammad Yousef Sefidan, Fatemeh Yeganeh Yekani, Mina Ghotaslou, Reza In vitro synergy of antibiotic combinations against planktonic and biofilm Pseudomonas aeruginosa GMS Hygiene and Infection Control antibiofilm combination therapy Pseudomonas aeruginosa synergetic effects |
| title | In vitro synergy of antibiotic combinations against planktonic and biofilm Pseudomonas aeruginosa |
| title_full | In vitro synergy of antibiotic combinations against planktonic and biofilm Pseudomonas aeruginosa |
| title_fullStr | In vitro synergy of antibiotic combinations against planktonic and biofilm Pseudomonas aeruginosa |
| title_full_unstemmed | In vitro synergy of antibiotic combinations against planktonic and biofilm Pseudomonas aeruginosa |
| title_short | In vitro synergy of antibiotic combinations against planktonic and biofilm Pseudomonas aeruginosa |
| title_sort | in vitro synergy of antibiotic combinations against planktonic and biofilm pseudomonas aeruginosa |
| topic | antibiofilm combination therapy Pseudomonas aeruginosa synergetic effects |
| url | http://www.egms.de/static/en/journals/dgkh/2017-12/dgkh000302.shtml |
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