The application of existing genotoxicity methodologies for grouping of nanomaterials: towards an integrated approach to testing and assessment
Abstract The incorporation of nanomaterials (NMs) in consumer products has proven to be highly valuable in many sectors. Unfortunately, however, the same nano specific physicochemical properties, which make these material attractive, might also contribute to hazards for people exposed to these mater...
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| Format: | Article |
| Language: | English |
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BMC
2022-05-01
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| Series: | Particle and Fibre Toxicology |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s12989-022-00476-9 |
| _version_ | 1811308207943974912 |
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| author | Rachel Verdon Vicki Stone Fiona Murphy Emily Christopher Helinor Johnston Shareen Doak Ulla Vogel Andrea Haase Ali Kermanizadeh |
| author_facet | Rachel Verdon Vicki Stone Fiona Murphy Emily Christopher Helinor Johnston Shareen Doak Ulla Vogel Andrea Haase Ali Kermanizadeh |
| author_sort | Rachel Verdon |
| collection | DOAJ |
| description | Abstract The incorporation of nanomaterials (NMs) in consumer products has proven to be highly valuable in many sectors. Unfortunately, however, the same nano specific physicochemical properties, which make these material attractive, might also contribute to hazards for people exposed to these materials. The physicochemical properties of NMs will impact their interaction with biological surroundings and influence their fate and their potential adverse effects such as genotoxicity. Due to the large and expanding number of NMs produced, their availability in different nanoforms (NFs) and their utilization in various formats, it is impossible for risk assessment to be conducted on an individual NF basis. Alternative methods, such as grouping are needed for streamlining hazard assessment. The GRACIOUS Framework provides a logical and science evidenced approach to group similar NFs, allowing read-across of hazard information from source NFs (or non-NFs) with adequate hazard data to target NFs that lack such data. Here, we propose a simple three-tiered testing strategy to gather evidence to determine whether different NFs are sufficiently similar with respect to their potential to induce genotoxicity, in order to be grouped. The tiered testing strategy includes simple in vitro models as well as a number of alternative more complex multi-cellular in vitro models to allow for a better understanding of secondary NM-induced DNA damage, something that has been more appropriate in vivo until recently. |
| first_indexed | 2024-04-13T09:19:12Z |
| format | Article |
| id | doaj.art-a3e1837ca8fe42b89707349e5fd7bc9b |
| institution | Directory Open Access Journal |
| issn | 1743-8977 |
| language | English |
| last_indexed | 2024-04-13T09:19:12Z |
| publishDate | 2022-05-01 |
| publisher | BMC |
| record_format | Article |
| series | Particle and Fibre Toxicology |
| spelling | doaj.art-a3e1837ca8fe42b89707349e5fd7bc9b2022-12-22T02:52:40ZengBMCParticle and Fibre Toxicology1743-89772022-05-011911910.1186/s12989-022-00476-9The application of existing genotoxicity methodologies for grouping of nanomaterials: towards an integrated approach to testing and assessmentRachel Verdon0Vicki Stone1Fiona Murphy2Emily Christopher3Helinor Johnston4Shareen Doak5Ulla Vogel6Andrea Haase7Ali Kermanizadeh8Nano Safety Research Group, Heriot-Watt UniversityNano Safety Research Group, Heriot-Watt UniversityNano Safety Research Group, Heriot-Watt UniversityNano Safety Research Group, Heriot-Watt UniversityNano Safety Research Group, Heriot-Watt UniversityInstitute of Life Science, Swansea University Medical SchoolNational Research Centre for the Working EnvironmentDepartment of Chemicals and Product Safety, German Federal Institute for Risk Assessment (BfR)Human Sciences Research Centre, University of DerbyAbstract The incorporation of nanomaterials (NMs) in consumer products has proven to be highly valuable in many sectors. Unfortunately, however, the same nano specific physicochemical properties, which make these material attractive, might also contribute to hazards for people exposed to these materials. The physicochemical properties of NMs will impact their interaction with biological surroundings and influence their fate and their potential adverse effects such as genotoxicity. Due to the large and expanding number of NMs produced, their availability in different nanoforms (NFs) and their utilization in various formats, it is impossible for risk assessment to be conducted on an individual NF basis. Alternative methods, such as grouping are needed for streamlining hazard assessment. The GRACIOUS Framework provides a logical and science evidenced approach to group similar NFs, allowing read-across of hazard information from source NFs (or non-NFs) with adequate hazard data to target NFs that lack such data. Here, we propose a simple three-tiered testing strategy to gather evidence to determine whether different NFs are sufficiently similar with respect to their potential to induce genotoxicity, in order to be grouped. The tiered testing strategy includes simple in vitro models as well as a number of alternative more complex multi-cellular in vitro models to allow for a better understanding of secondary NM-induced DNA damage, something that has been more appropriate in vivo until recently.https://doi.org/10.1186/s12989-022-00476-9NanomaterialsGenotoxicityGroupingTiered testing strategyAlternative physiological multi-cellular models |
| spellingShingle | Rachel Verdon Vicki Stone Fiona Murphy Emily Christopher Helinor Johnston Shareen Doak Ulla Vogel Andrea Haase Ali Kermanizadeh The application of existing genotoxicity methodologies for grouping of nanomaterials: towards an integrated approach to testing and assessment Particle and Fibre Toxicology Nanomaterials Genotoxicity Grouping Tiered testing strategy Alternative physiological multi-cellular models |
| title | The application of existing genotoxicity methodologies for grouping of nanomaterials: towards an integrated approach to testing and assessment |
| title_full | The application of existing genotoxicity methodologies for grouping of nanomaterials: towards an integrated approach to testing and assessment |
| title_fullStr | The application of existing genotoxicity methodologies for grouping of nanomaterials: towards an integrated approach to testing and assessment |
| title_full_unstemmed | The application of existing genotoxicity methodologies for grouping of nanomaterials: towards an integrated approach to testing and assessment |
| title_short | The application of existing genotoxicity methodologies for grouping of nanomaterials: towards an integrated approach to testing and assessment |
| title_sort | application of existing genotoxicity methodologies for grouping of nanomaterials towards an integrated approach to testing and assessment |
| topic | Nanomaterials Genotoxicity Grouping Tiered testing strategy Alternative physiological multi-cellular models |
| url | https://doi.org/10.1186/s12989-022-00476-9 |
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