Secondary metabolites, hormonal homeostasis, and antioxidant enzymes of Moringa oleifera in response to white or violet Light Emitting Diodes (LEDs) combined with cytokinins under tissue culture conditions
The present study was carried out between 2021 and 2022 at the tissue culture lab and experimental station of the vegetable and floriculture department, faculty of agriculture, Mansoura University. Using mature seeds, Moringa oleifera is propagated on a large scale and in rapid succession by in vit...
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| Format: | Article |
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AcademicPres
2022-12-01
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| Series: | Notulae Botanicae Horti Agrobotanici Cluj-Napoca |
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| Online Access: | https://www.notulaebotanicae.ro/index.php/nbha/article/view/12879 |
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| author | Lina M. ABU-ZIADA Samia A. HAROUN Rasha M.E. GAMEL Mohamed A. ABBAS Mahmoud R. SOFY Heba Y. EL-BANNA |
| author_facet | Lina M. ABU-ZIADA Samia A. HAROUN Rasha M.E. GAMEL Mohamed A. ABBAS Mahmoud R. SOFY Heba Y. EL-BANNA |
| author_sort | Lina M. ABU-ZIADA |
| collection | DOAJ |
| description |
The present study was carried out between 2021 and 2022 at the tissue culture lab and experimental station of the vegetable and floriculture department, faculty of agriculture, Mansoura University. Using mature seeds, Moringa oleifera is propagated on a large scale and in rapid succession by in vitro culture. Two types of cytokinin: 6-benzylaminopurin (BAP), and thidiauron (TDZ) singly at three different concentrations (0, 1, or 2 mg/L), were used, as well as light-emitting diode systems (LEDs) (white as control and violet; the combination of red and blue; 1:1). After incubation for 30 days, the obtained results showed that the survival percentage increased by the treatments with the maximum value (85%) by MS medium supplemented with 2 mg/L TDZ, under violet LEDs illumination. However, the addition of thidiazuron (TDZ) to the medium did not propagate shoot, and this treatment recorded 100% callus formation other than BAP. The maximum number of axillary shoots per explant and the number of leaves recorded in the MS medium contained 2 mg/L BAP under violet LEDs. Compared to the control values, all the used treatments generally caused surprisingly stimulating the determined antioxidant enzymes and compounds (peroxidase, polyphenol oxidase &catalase and phenols, flavonoids, anthocyanin& ascorbic acid). Concerning the phytohormone content of the hormone-free medium (control), IAA, kinetin, and zeatin increased. Meanwhile, GA3 and ABA decreased with the used treatments.
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| first_indexed | 2024-04-11T05:56:38Z |
| format | Article |
| id | doaj.art-a3e3588dd94048faa62d494075c299d5 |
| institution | Directory Open Access Journal |
| issn | 0255-965X 1842-4309 |
| language | English |
| last_indexed | 2024-04-11T05:56:38Z |
| publishDate | 2022-12-01 |
| publisher | AcademicPres |
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| series | Notulae Botanicae Horti Agrobotanici Cluj-Napoca |
| spelling | doaj.art-a3e3588dd94048faa62d494075c299d52022-12-22T04:41:53ZengAcademicPresNotulae Botanicae Horti Agrobotanici Cluj-Napoca0255-965X1842-43092022-12-0150410.15835/nbha50312879Secondary metabolites, hormonal homeostasis, and antioxidant enzymes of Moringa oleifera in response to white or violet Light Emitting Diodes (LEDs) combined with cytokinins under tissue culture conditionsLina M. ABU-ZIADA0Samia A. HAROUN1Rasha M.E. GAMEL2Mohamed A. ABBAS3Mahmoud R. SOFY4Heba Y. EL-BANNA5Mansoura University, Faculty of Science, Botany Department, 35516 MansouraMansoura University, Faculty of Science, Botany Department, 35516 MansouraMansoura University, Faculty of Science, Botany Department, 35516 MansouraMansoura University, Faculty of Science, Botany Department, 35516 MansouraAl Azhar University, Faculty of Science, Botany and Microbiology Department, 11884 Nasr City, CairoMansoura University, Faculty of Agriculture, Vegetable and Floriculture Department, 35516 Mansoura The present study was carried out between 2021 and 2022 at the tissue culture lab and experimental station of the vegetable and floriculture department, faculty of agriculture, Mansoura University. Using mature seeds, Moringa oleifera is propagated on a large scale and in rapid succession by in vitro culture. Two types of cytokinin: 6-benzylaminopurin (BAP), and thidiauron (TDZ) singly at three different concentrations (0, 1, or 2 mg/L), were used, as well as light-emitting diode systems (LEDs) (white as control and violet; the combination of red and blue; 1:1). After incubation for 30 days, the obtained results showed that the survival percentage increased by the treatments with the maximum value (85%) by MS medium supplemented with 2 mg/L TDZ, under violet LEDs illumination. However, the addition of thidiazuron (TDZ) to the medium did not propagate shoot, and this treatment recorded 100% callus formation other than BAP. The maximum number of axillary shoots per explant and the number of leaves recorded in the MS medium contained 2 mg/L BAP under violet LEDs. Compared to the control values, all the used treatments generally caused surprisingly stimulating the determined antioxidant enzymes and compounds (peroxidase, polyphenol oxidase &catalase and phenols, flavonoids, anthocyanin& ascorbic acid). Concerning the phytohormone content of the hormone-free medium (control), IAA, kinetin, and zeatin increased. Meanwhile, GA3 and ABA decreased with the used treatments. https://www.notulaebotanicae.ro/index.php/nbha/article/view/12879benzyl aminopurinelight-emitting diode systems (LEDs)Moringa oleiferasecondary metabolitesthidiazurontissue culture |
| spellingShingle | Lina M. ABU-ZIADA Samia A. HAROUN Rasha M.E. GAMEL Mohamed A. ABBAS Mahmoud R. SOFY Heba Y. EL-BANNA Secondary metabolites, hormonal homeostasis, and antioxidant enzymes of Moringa oleifera in response to white or violet Light Emitting Diodes (LEDs) combined with cytokinins under tissue culture conditions Notulae Botanicae Horti Agrobotanici Cluj-Napoca benzyl aminopurine light-emitting diode systems (LEDs) Moringa oleifera secondary metabolites thidiazuron tissue culture |
| title | Secondary metabolites, hormonal homeostasis, and antioxidant enzymes of Moringa oleifera in response to white or violet Light Emitting Diodes (LEDs) combined with cytokinins under tissue culture conditions |
| title_full | Secondary metabolites, hormonal homeostasis, and antioxidant enzymes of Moringa oleifera in response to white or violet Light Emitting Diodes (LEDs) combined with cytokinins under tissue culture conditions |
| title_fullStr | Secondary metabolites, hormonal homeostasis, and antioxidant enzymes of Moringa oleifera in response to white or violet Light Emitting Diodes (LEDs) combined with cytokinins under tissue culture conditions |
| title_full_unstemmed | Secondary metabolites, hormonal homeostasis, and antioxidant enzymes of Moringa oleifera in response to white or violet Light Emitting Diodes (LEDs) combined with cytokinins under tissue culture conditions |
| title_short | Secondary metabolites, hormonal homeostasis, and antioxidant enzymes of Moringa oleifera in response to white or violet Light Emitting Diodes (LEDs) combined with cytokinins under tissue culture conditions |
| title_sort | secondary metabolites hormonal homeostasis and antioxidant enzymes of moringa oleifera in response to white or violet light emitting diodes leds combined with cytokinins under tissue culture conditions |
| topic | benzyl aminopurine light-emitting diode systems (LEDs) Moringa oleifera secondary metabolites thidiazuron tissue culture |
| url | https://www.notulaebotanicae.ro/index.php/nbha/article/view/12879 |
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