Morphogenetic Potential of Tomato (Lycopersicon esculentum) cv. ‘Arka Ahuti’ to Plant Growth Regulators
A highly reproducible in vitro regeneration method for tomato (Lycopersicon esculentum Mill.) cultivar ‘Arka Ahuti’ was established by using hypocotyl, leaf and cotyledon explants from in vitro raised seedlings on Murashige and Skoog medium supplemented with different concentrations and combinations...
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| Format: | Article |
| Language: | English |
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Society of Land Measurements and Cadastre from Transylvania (SMTCT)
2013-05-01
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| Series: | Notulae Scientia Biologicae |
| Online Access: | http://www.notulaebiologicae.ro/index.php/nsb/article/view/9037 |
| _version_ | 1828745184416890880 |
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| author | Kanakapura K. NAMITHA Pradeep S. NEGI |
| author_facet | Kanakapura K. NAMITHA Pradeep S. NEGI |
| author_sort | Kanakapura K. NAMITHA |
| collection | DOAJ |
| description | A highly reproducible in vitro regeneration method for tomato (Lycopersicon esculentum Mill.) cultivar ‘Arka Ahuti’ was established by using hypocotyl, leaf and cotyledon explants from in vitro raised seedlings on Murashige and Skoog medium supplemented with different concentrations and combinations of hormones 6-Benzylamino purine (2 to 4 mg/L) and Indole-3-acetic acid (0.1 to 1 mg/L). The medium supplemented with 2 mg/L 6-benzylamino purine and 0.1 mg/L indole-3-acetic acid was found to be the best for inducing direct shoot regeneration and multiple shoots per explant from hypocotyl explants. Callus induction was observed in all the explants and regeneration of shoots was also promoted by all these combinations. Shoots were transferred to the elongation medium which also induced 100% rooting. After hardening, plants were transferred to soil. Thus, a tissue culture base line was established for ‘Arka Ahuti’ cultivar of tomato for obtaining direct regeneration using hypocotyl, leaf and cotyledon as explants. |
| first_indexed | 2024-04-14T03:57:24Z |
| format | Article |
| id | doaj.art-a3e655fd9e01437b9f6ff37f670e1ab1 |
| institution | Directory Open Access Journal |
| issn | 2067-3205 2067-3264 |
| language | English |
| last_indexed | 2024-04-14T03:57:24Z |
| publishDate | 2013-05-01 |
| publisher | Society of Land Measurements and Cadastre from Transylvania (SMTCT) |
| record_format | Article |
| series | Notulae Scientia Biologicae |
| spelling | doaj.art-a3e655fd9e01437b9f6ff37f670e1ab12022-12-22T02:13:42ZengSociety of Land Measurements and Cadastre from Transylvania (SMTCT)Notulae Scientia Biologicae2067-32052067-32642013-05-015222022510.15835/nsb5290377943Morphogenetic Potential of Tomato (Lycopersicon esculentum) cv. ‘Arka Ahuti’ to Plant Growth RegulatorsKanakapura K. NAMITHA0Pradeep S. NEGI1Fruit and Vegetable Technology Department, CSIR-Central Food Technological Research Institute, Mysore-570020Fruit and Vegetable Technology Department, CSIR-Central Food Technological Research Institute, Mysore-570020A highly reproducible in vitro regeneration method for tomato (Lycopersicon esculentum Mill.) cultivar ‘Arka Ahuti’ was established by using hypocotyl, leaf and cotyledon explants from in vitro raised seedlings on Murashige and Skoog medium supplemented with different concentrations and combinations of hormones 6-Benzylamino purine (2 to 4 mg/L) and Indole-3-acetic acid (0.1 to 1 mg/L). The medium supplemented with 2 mg/L 6-benzylamino purine and 0.1 mg/L indole-3-acetic acid was found to be the best for inducing direct shoot regeneration and multiple shoots per explant from hypocotyl explants. Callus induction was observed in all the explants and regeneration of shoots was also promoted by all these combinations. Shoots were transferred to the elongation medium which also induced 100% rooting. After hardening, plants were transferred to soil. Thus, a tissue culture base line was established for ‘Arka Ahuti’ cultivar of tomato for obtaining direct regeneration using hypocotyl, leaf and cotyledon as explants.http://www.notulaebiologicae.ro/index.php/nsb/article/view/9037 |
| spellingShingle | Kanakapura K. NAMITHA Pradeep S. NEGI Morphogenetic Potential of Tomato (Lycopersicon esculentum) cv. ‘Arka Ahuti’ to Plant Growth Regulators Notulae Scientia Biologicae |
| title | Morphogenetic Potential of Tomato (Lycopersicon esculentum) cv. ‘Arka Ahuti’ to Plant Growth Regulators |
| title_full | Morphogenetic Potential of Tomato (Lycopersicon esculentum) cv. ‘Arka Ahuti’ to Plant Growth Regulators |
| title_fullStr | Morphogenetic Potential of Tomato (Lycopersicon esculentum) cv. ‘Arka Ahuti’ to Plant Growth Regulators |
| title_full_unstemmed | Morphogenetic Potential of Tomato (Lycopersicon esculentum) cv. ‘Arka Ahuti’ to Plant Growth Regulators |
| title_short | Morphogenetic Potential of Tomato (Lycopersicon esculentum) cv. ‘Arka Ahuti’ to Plant Growth Regulators |
| title_sort | morphogenetic potential of tomato lycopersicon esculentum cv arka ahuti to plant growth regulators |
| url | http://www.notulaebiologicae.ro/index.php/nsb/article/view/9037 |
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