Colostrum-Induced Temporary Changes in the Expression of Proteins Regulating the Epithelial Barrier Function in the Intestine
The intestinal wall and epithelial cells are interconnected by numerous intercellular junctions. Colostrum (Col), in its natural form, is a secretion of the mammary gland of mammals at the end of pregnancy and up to 72 h after birth. Recently, it has been used as a biologically active dietary supple...
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| Format: | Article |
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MDPI AG
2022-02-01
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| Series: | Foods |
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| Online Access: | https://www.mdpi.com/2304-8158/11/5/685 |
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| author | Sylwia Rzeszotek Grzegorz Trybek Maciej Tarnowski Karol Serwin Aleksandra Jaroń Gabriela Schneider Agnieszka Kolasa Barbara Wiszniewska |
| author_facet | Sylwia Rzeszotek Grzegorz Trybek Maciej Tarnowski Karol Serwin Aleksandra Jaroń Gabriela Schneider Agnieszka Kolasa Barbara Wiszniewska |
| author_sort | Sylwia Rzeszotek |
| collection | DOAJ |
| description | The intestinal wall and epithelial cells are interconnected by numerous intercellular junctions. Colostrum (Col), in its natural form, is a secretion of the mammary gland of mammals at the end of pregnancy and up to 72 h after birth. Recently, it has been used as a biologically active dietary supplement with a high content of lactoferrin (Lf). Lf, a glycoprotein with a broad spectrum of activity, is becoming more popular in health-promoting supplements. This study aims to investigate whether Col supplementation can affect small and large intestine morphology by modulating the expression of selected proteins involved in tissue integrity. We examined the thickness of the epithelium, and the length of the microvilli, and assessed the expression of <i>CDH1</i>, <i>CDH2</i>, <i>CTNNB</i>, <i>CX43</i>, <i>VCL</i>, <i>OCLN</i>, <i>HP</i>, <i>MYH9</i>, and <i>ACTG2</i> gene levels using qRT-PCR and at the protein level using IHC. Additionally, to evaluate whether the effect of Col supplementation is temporary or persistent, we performed all analyses on tissues collected from animals receiving Col for 1, 3, or 6 months. We noticed a decrease in <i>CDH1</i> and <i>CDH2</i> expression, especially after 3 months of supplementation in the large intestine and in <i>CTNNB</i> in the small intestine as well as increased levels of <i>CX43</i> and <i>CTNNB1</i> in the small intestine. The present data indicate that Col can temporarily alter some components of the cell adhesion molecules involved in the formation of the cellular barrier. |
| first_indexed | 2024-03-09T20:40:04Z |
| format | Article |
| id | doaj.art-a3e813274b3e42fd91207e41259e0e4d |
| institution | Directory Open Access Journal |
| issn | 2304-8158 |
| language | English |
| last_indexed | 2024-03-09T20:40:04Z |
| publishDate | 2022-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Foods |
| spelling | doaj.art-a3e813274b3e42fd91207e41259e0e4d2023-11-23T23:00:56ZengMDPI AGFoods2304-81582022-02-0111568510.3390/foods11050685Colostrum-Induced Temporary Changes in the Expression of Proteins Regulating the Epithelial Barrier Function in the IntestineSylwia Rzeszotek0Grzegorz Trybek1Maciej Tarnowski2Karol Serwin3Aleksandra Jaroń4Gabriela Schneider5Agnieszka Kolasa6Barbara Wiszniewska7Department of Histology and Embryology, Pomeranian Medical University in Szczecin, 72 Powstańców Wlkp., 70-111 Szczecin, PolandDepartment of Oral Surgery, Pomeranian Medical University in Szczecin, 72 Powstańców Wlkp., 70-111 Szczecin, PolandDepartment of Physiology, Pomeranian Medical University in Szczecin, 72 Powstańców Wlkp., 70-111 Szczecin, PolandDepartment of Infectious Tropical Diseases and Immune Deficiency, Pomeranian Medical University in Szczecin, Arkońska 4, 71-455 Szczecin, PolandDepartment of Oral Surgery, Pomeranian Medical University in Szczecin, 72 Powstańców Wlkp., 70-111 Szczecin, PolandUofL Health-Brown Cancer Center and Division of Medical Oncology and Hematology, Department of Medicine, University of Louisville, Louisville, KY 40202, USADepartment of Histology and Embryology, Pomeranian Medical University in Szczecin, 72 Powstańców Wlkp., 70-111 Szczecin, PolandDepartment of Histology and Embryology, Pomeranian Medical University in Szczecin, 72 Powstańców Wlkp., 70-111 Szczecin, PolandThe intestinal wall and epithelial cells are interconnected by numerous intercellular junctions. Colostrum (Col), in its natural form, is a secretion of the mammary gland of mammals at the end of pregnancy and up to 72 h after birth. Recently, it has been used as a biologically active dietary supplement with a high content of lactoferrin (Lf). Lf, a glycoprotein with a broad spectrum of activity, is becoming more popular in health-promoting supplements. This study aims to investigate whether Col supplementation can affect small and large intestine morphology by modulating the expression of selected proteins involved in tissue integrity. We examined the thickness of the epithelium, and the length of the microvilli, and assessed the expression of <i>CDH1</i>, <i>CDH2</i>, <i>CTNNB</i>, <i>CX43</i>, <i>VCL</i>, <i>OCLN</i>, <i>HP</i>, <i>MYH9</i>, and <i>ACTG2</i> gene levels using qRT-PCR and at the protein level using IHC. Additionally, to evaluate whether the effect of Col supplementation is temporary or persistent, we performed all analyses on tissues collected from animals receiving Col for 1, 3, or 6 months. We noticed a decrease in <i>CDH1</i> and <i>CDH2</i> expression, especially after 3 months of supplementation in the large intestine and in <i>CTNNB</i> in the small intestine as well as increased levels of <i>CX43</i> and <i>CTNNB1</i> in the small intestine. The present data indicate that Col can temporarily alter some components of the cell adhesion molecules involved in the formation of the cellular barrier.https://www.mdpi.com/2304-8158/11/5/685colostrumlactoferrinintestinecellular junctions |
| spellingShingle | Sylwia Rzeszotek Grzegorz Trybek Maciej Tarnowski Karol Serwin Aleksandra Jaroń Gabriela Schneider Agnieszka Kolasa Barbara Wiszniewska Colostrum-Induced Temporary Changes in the Expression of Proteins Regulating the Epithelial Barrier Function in the Intestine Foods colostrum lactoferrin intestine cellular junctions |
| title | Colostrum-Induced Temporary Changes in the Expression of Proteins Regulating the Epithelial Barrier Function in the Intestine |
| title_full | Colostrum-Induced Temporary Changes in the Expression of Proteins Regulating the Epithelial Barrier Function in the Intestine |
| title_fullStr | Colostrum-Induced Temporary Changes in the Expression of Proteins Regulating the Epithelial Barrier Function in the Intestine |
| title_full_unstemmed | Colostrum-Induced Temporary Changes in the Expression of Proteins Regulating the Epithelial Barrier Function in the Intestine |
| title_short | Colostrum-Induced Temporary Changes in the Expression of Proteins Regulating the Epithelial Barrier Function in the Intestine |
| title_sort | colostrum induced temporary changes in the expression of proteins regulating the epithelial barrier function in the intestine |
| topic | colostrum lactoferrin intestine cellular junctions |
| url | https://www.mdpi.com/2304-8158/11/5/685 |
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