EXPRESSION OF UBIQUITIN SPECIFIC PEPTIDASE GENES IN IRE1 KNOCKDOWN U87 GLIOMA CELLS UPON GLUCOSE DEPRIVATION
We have studied the effect of glucose deprivation on the expression of genes encoding for ubiquitin specific peptidases — USP and autophagy related 7 ATG7 in U87 glioma cells in relation to inhibition of inositol requiring enzyme-1 (IRE1). It was shown that glucose deprivation was downregulated the...
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| Format: | Article |
| Language: | English |
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National Academy of Sciences of Ukraine, Palladin Institute of Biochemistry
2016-10-01
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| Series: | Biotechnologia Acta |
| Subjects: | |
| Online Access: | http://biotechnology.kiev.ua/images/5_2016/minchenko_5_2016.pdf |
| _version_ | 1797762670316748800 |
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| author | O. H. Minchenko O. O. Riabovol O. V. Halkin S. V. Danilovskyi D. O. Minchenko O. O. Ratushna |
| author_facet | O. H. Minchenko O. O. Riabovol O. V. Halkin S. V. Danilovskyi D. O. Minchenko O. O. Ratushna |
| author_sort | O. H. Minchenko |
| collection | DOAJ |
| description | We have studied the effect of glucose deprivation on the expression of genes encoding for ubiquitin specific peptidases — USP and autophagy related 7 ATG7 in U87 glioma cells in relation to inhibition of inositol requiring enzyme-1 (IRE1). It was shown that glucose deprivation was downregulated the expression of USP1 and USP10 genes and up-regulated USP4 and USP25 genes in control (transfected by empty vector) glioma cells. At the same time, the expression level of USP14, USP22, and ATG7 genes in these cells did not significantly change upon glucose deprivation condition. Inhibition of ІRE1 signaling enzyme function in U87 glioma cells modified effect of glucose deprivation on the expression of most studied genes. Therefore, glucose deprivation affected the expression level of most ubiquitin specific peptidases genes in relation to the functional activity of IRE1 enzyme, which controls cell proliferation and tumor growth as a central mediator of endoplasmic reticulum stress. |
| first_indexed | 2024-03-12T19:31:43Z |
| format | Article |
| id | doaj.art-a3ecf959788b49acb1e7090d1ef98b11 |
| institution | Directory Open Access Journal |
| issn | 2410-7751 2410-776X |
| language | English |
| last_indexed | 2024-03-12T19:31:43Z |
| publishDate | 2016-10-01 |
| publisher | National Academy of Sciences of Ukraine, Palladin Institute of Biochemistry |
| record_format | Article |
| series | Biotechnologia Acta |
| spelling | doaj.art-a3ecf959788b49acb1e7090d1ef98b112023-08-02T04:30:04ZengNational Academy of Sciences of Ukraine, Palladin Institute of BiochemistryBiotechnologia Acta2410-77512410-776X2016-10-019571710.15407/biotech9.05.007EXPRESSION OF UBIQUITIN SPECIFIC PEPTIDASE GENES IN IRE1 KNOCKDOWN U87 GLIOMA CELLS UPON GLUCOSE DEPRIVATIONO. H. Minchenko0O. O. Riabovol1O. V. Halkin2S. V. Danilovskyi3D. O. Minchenko4O. O. Ratushna5Palladin Institute of Biochemistry of the National Academy of Sciences of Ukraine, KyivPalladin Institute of Biochemistry of the National Academy of Sciences of Ukraine, KyivPalladin Institute of Biochemistry of the National Academy of Sciences of Ukraine, KyivPalladin Institute of Biochemistry of the National Academy of Sciences of Ukraine, KyivPalladin Institute of Biochemistry of the National Academy of Sciences of Ukraine, KyivBohomolets National Medical University, Kyiv Palladin Institute of Biochemistry of the National Academy of Sciences of Ukraine, KyivWe have studied the effect of glucose deprivation on the expression of genes encoding for ubiquitin specific peptidases — USP and autophagy related 7 ATG7 in U87 glioma cells in relation to inhibition of inositol requiring enzyme-1 (IRE1). It was shown that glucose deprivation was downregulated the expression of USP1 and USP10 genes and up-regulated USP4 and USP25 genes in control (transfected by empty vector) glioma cells. At the same time, the expression level of USP14, USP22, and ATG7 genes in these cells did not significantly change upon glucose deprivation condition. Inhibition of ІRE1 signaling enzyme function in U87 glioma cells modified effect of glucose deprivation on the expression of most studied genes. Therefore, glucose deprivation affected the expression level of most ubiquitin specific peptidases genes in relation to the functional activity of IRE1 enzyme, which controls cell proliferation and tumor growth as a central mediator of endoplasmic reticulum stress.http://biotechnology.kiev.ua/images/5_2016/minchenko_5_2016.pdfmRNA expressionUSP genesIRE1 inhibitionglucose deprivationU87 glioma cells |
| spellingShingle | O. H. Minchenko O. O. Riabovol O. V. Halkin S. V. Danilovskyi D. O. Minchenko O. O. Ratushna EXPRESSION OF UBIQUITIN SPECIFIC PEPTIDASE GENES IN IRE1 KNOCKDOWN U87 GLIOMA CELLS UPON GLUCOSE DEPRIVATION Biotechnologia Acta mRNA expression USP genes IRE1 inhibition glucose deprivation U87 glioma cells |
| title | EXPRESSION OF UBIQUITIN SPECIFIC PEPTIDASE GENES IN IRE1 KNOCKDOWN U87 GLIOMA CELLS UPON GLUCOSE DEPRIVATION |
| title_full | EXPRESSION OF UBIQUITIN SPECIFIC PEPTIDASE GENES IN IRE1 KNOCKDOWN U87 GLIOMA CELLS UPON GLUCOSE DEPRIVATION |
| title_fullStr | EXPRESSION OF UBIQUITIN SPECIFIC PEPTIDASE GENES IN IRE1 KNOCKDOWN U87 GLIOMA CELLS UPON GLUCOSE DEPRIVATION |
| title_full_unstemmed | EXPRESSION OF UBIQUITIN SPECIFIC PEPTIDASE GENES IN IRE1 KNOCKDOWN U87 GLIOMA CELLS UPON GLUCOSE DEPRIVATION |
| title_short | EXPRESSION OF UBIQUITIN SPECIFIC PEPTIDASE GENES IN IRE1 KNOCKDOWN U87 GLIOMA CELLS UPON GLUCOSE DEPRIVATION |
| title_sort | expression of ubiquitin specific peptidase genes in ire1 knockdown u87 glioma cells upon glucose deprivation |
| topic | mRNA expression USP genes IRE1 inhibition glucose deprivation U87 glioma cells |
| url | http://biotechnology.kiev.ua/images/5_2016/minchenko_5_2016.pdf |
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