Crosstalk between Thyroid Hormone and Corticosteroid Signaling Targets Cell Proliferation in <i>Xenopus tropicalis</i> Tadpole Liver
Thyroid hormones (TH) and glucocorticoids (GC) are involved in numerous developmental and physiological processes. The effects of individual hormones are well documented, but little is known about the joint actions of the two hormones. To decipher the crosstalk between these two hormonal pathways, w...
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MDPI AG
2022-11-01
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| Series: | International Journal of Molecular Sciences |
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| author | Muriel Rigolet Nicolas Buisine Marylou Scharwatt Evelyne Duvernois-Berthet Daniel R. Buchholz Laurent M. Sachs |
| author_facet | Muriel Rigolet Nicolas Buisine Marylou Scharwatt Evelyne Duvernois-Berthet Daniel R. Buchholz Laurent M. Sachs |
| author_sort | Muriel Rigolet |
| collection | DOAJ |
| description | Thyroid hormones (TH) and glucocorticoids (GC) are involved in numerous developmental and physiological processes. The effects of individual hormones are well documented, but little is known about the joint actions of the two hormones. To decipher the crosstalk between these two hormonal pathways, we conducted a transcriptional analysis of genes regulated by TH, GC, or both hormones together in liver of <i>Xenopus tropicalis</i> tadpoles using RNA-Seq. Among the differentially expressed genes (DE), 70.5% were regulated by TH only, 0.87% by GC only, and 15% by crosstalk between the two hormones. Gene ontology analysis of the crosstalk-regulated genes identified terms referring to DNA replication, DNA repair, and cell-cycle regulation. Biological network analysis identified groups of genes targeted by the hormonal crosstalk and corroborated the gene ontology analysis. Specifically, we found two groups of functionally linked genes (chains) mainly composed of crosstalk-regulated hubs (highly interactive genes), and a large subnetwork centred around the crosstalk-regulated genes <i>psmb6</i> and <i>cdc7</i>. Most of the genes in the chains are involved in cell-cycle regulation, as are <i>psmb6</i> and <i>cdc7</i>, which regulate the G2/M transition. Thus, the biological action of these two hormonal pathways acting together in the liver targets cell-cycle regulation. |
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| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-09T18:18:51Z |
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| series | International Journal of Molecular Sciences |
| spelling | doaj.art-a3eef7ce55e948c3ab2ee0b82f5176f42023-11-24T08:32:12ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672022-11-0123221371510.3390/ijms232213715Crosstalk between Thyroid Hormone and Corticosteroid Signaling Targets Cell Proliferation in <i>Xenopus tropicalis</i> Tadpole LiverMuriel Rigolet0Nicolas Buisine1Marylou Scharwatt2Evelyne Duvernois-Berthet3Daniel R. Buchholz4Laurent M. Sachs5UMR PhyMA CNRS, Muséum National d’Histoire Naturelle, 75005 Paris, FranceUMR PhyMA CNRS, Muséum National d’Histoire Naturelle, 75005 Paris, FranceUMR PhyMA CNRS, Muséum National d’Histoire Naturelle, 75005 Paris, FranceUMR PhyMA CNRS, Muséum National d’Histoire Naturelle, 75005 Paris, FranceDepartment of Biological Sciences, University of Cincinnati, Cincinnati, OH 45221, USAUMR PhyMA CNRS, Muséum National d’Histoire Naturelle, 75005 Paris, FranceThyroid hormones (TH) and glucocorticoids (GC) are involved in numerous developmental and physiological processes. The effects of individual hormones are well documented, but little is known about the joint actions of the two hormones. To decipher the crosstalk between these two hormonal pathways, we conducted a transcriptional analysis of genes regulated by TH, GC, or both hormones together in liver of <i>Xenopus tropicalis</i> tadpoles using RNA-Seq. Among the differentially expressed genes (DE), 70.5% were regulated by TH only, 0.87% by GC only, and 15% by crosstalk between the two hormones. Gene ontology analysis of the crosstalk-regulated genes identified terms referring to DNA replication, DNA repair, and cell-cycle regulation. Biological network analysis identified groups of genes targeted by the hormonal crosstalk and corroborated the gene ontology analysis. Specifically, we found two groups of functionally linked genes (chains) mainly composed of crosstalk-regulated hubs (highly interactive genes), and a large subnetwork centred around the crosstalk-regulated genes <i>psmb6</i> and <i>cdc7</i>. Most of the genes in the chains are involved in cell-cycle regulation, as are <i>psmb6</i> and <i>cdc7</i>, which regulate the G2/M transition. Thus, the biological action of these two hormonal pathways acting together in the liver targets cell-cycle regulation.https://www.mdpi.com/1422-0067/23/22/13715thyroid hormoneglucocorticoidhormonal crosstalkmetamorphosis<i>Xenopus tropicalis</i> |
| spellingShingle | Muriel Rigolet Nicolas Buisine Marylou Scharwatt Evelyne Duvernois-Berthet Daniel R. Buchholz Laurent M. Sachs Crosstalk between Thyroid Hormone and Corticosteroid Signaling Targets Cell Proliferation in <i>Xenopus tropicalis</i> Tadpole Liver International Journal of Molecular Sciences thyroid hormone glucocorticoid hormonal crosstalk metamorphosis <i>Xenopus tropicalis</i> |
| title | Crosstalk between Thyroid Hormone and Corticosteroid Signaling Targets Cell Proliferation in <i>Xenopus tropicalis</i> Tadpole Liver |
| title_full | Crosstalk between Thyroid Hormone and Corticosteroid Signaling Targets Cell Proliferation in <i>Xenopus tropicalis</i> Tadpole Liver |
| title_fullStr | Crosstalk between Thyroid Hormone and Corticosteroid Signaling Targets Cell Proliferation in <i>Xenopus tropicalis</i> Tadpole Liver |
| title_full_unstemmed | Crosstalk between Thyroid Hormone and Corticosteroid Signaling Targets Cell Proliferation in <i>Xenopus tropicalis</i> Tadpole Liver |
| title_short | Crosstalk between Thyroid Hormone and Corticosteroid Signaling Targets Cell Proliferation in <i>Xenopus tropicalis</i> Tadpole Liver |
| title_sort | crosstalk between thyroid hormone and corticosteroid signaling targets cell proliferation in i xenopus tropicalis i tadpole liver |
| topic | thyroid hormone glucocorticoid hormonal crosstalk metamorphosis <i>Xenopus tropicalis</i> |
| url | https://www.mdpi.com/1422-0067/23/22/13715 |
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