Bone marrow mesenchymal stromal cells in a 3D system produce higher concentration of extracellular vesicles (EVs) with increased complexity and enhanced neuronal growth properties

Bone marrow mesenchymal stromal cells in a 3D system produce higher concentration of extracellular vesicles (EVs) with increased complexity and enhanced neuronal growth properties

Highlights Extracellular vesicles (EV) derived from 2D and 3D bone marrow mesenchymal stromal cells (BM-MSCs) demonstrate different characterization phenotype. Heterogeneity and concentration of BM-MSCs-derived extracellular vesicles are highly impacted by the culture conditions. 3D-derived EVs can...

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Bibliographic Details
Main Authors: Elmira Jalilian, Hamed Massoumi, Bianca Bigit, Sohil Amin, Eitan A. Katz, Victor H. Guaiquil, Khandaker N. Anwar, Peiman Hematti, Mark I. Rosenblatt, Ali R. Djalilian
Format: Article
Language:English
Published: BMC 2022-08-01
Series:Stem Cell Research & Therapy
Subjects:
Extracellular vesicles
Exosomes
Bone marrow mesenchymal stromal cell
3D culture
2D culture
Neuronal growth
Online Access:https://doi.org/10.1186/s13287-022-03128-z
Description
Summary:Highlights Extracellular vesicles (EV) derived from 2D and 3D bone marrow mesenchymal stromal cells (BM-MSCs) demonstrate different characterization phenotype. Heterogeneity and concentration of BM-MSCs-derived extracellular vesicles are highly impacted by the culture conditions. 3D-derived EVs can significantly enhance trigeminal nerves elongation and complexity compared to 2D-derived EVs. 3D culture conditions are highly efficient environment for upscale production of MSC-derived EVs with enhanced neuro-regeneration properties for corneal nerve regeneration.
ISSN:1757-6512

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