Bone marrow mesenchymal stromal cells in a 3D system produce higher concentration of extracellular vesicles (EVs) with increased complexity and enhanced neuronal growth properties
Highlights Extracellular vesicles (EV) derived from 2D and 3D bone marrow mesenchymal stromal cells (BM-MSCs) demonstrate different characterization phenotype. Heterogeneity and concentration of BM-MSCs-derived extracellular vesicles are highly impacted by the culture conditions. 3D-derived EVs can...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2022-08-01
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| Series: | Stem Cell Research & Therapy |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s13287-022-03128-z |
| _version_ | 1818498034469699584 |
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| author | Elmira Jalilian Hamed Massoumi Bianca Bigit Sohil Amin Eitan A. Katz Victor H. Guaiquil Khandaker N. Anwar Peiman Hematti Mark I. Rosenblatt Ali R. Djalilian |
| author_facet | Elmira Jalilian Hamed Massoumi Bianca Bigit Sohil Amin Eitan A. Katz Victor H. Guaiquil Khandaker N. Anwar Peiman Hematti Mark I. Rosenblatt Ali R. Djalilian |
| author_sort | Elmira Jalilian |
| collection | DOAJ |
| description | Highlights Extracellular vesicles (EV) derived from 2D and 3D bone marrow mesenchymal stromal cells (BM-MSCs) demonstrate different characterization phenotype. Heterogeneity and concentration of BM-MSCs-derived extracellular vesicles are highly impacted by the culture conditions. 3D-derived EVs can significantly enhance trigeminal nerves elongation and complexity compared to 2D-derived EVs. 3D culture conditions are highly efficient environment for upscale production of MSC-derived EVs with enhanced neuro-regeneration properties for corneal nerve regeneration. |
| first_indexed | 2024-12-10T18:52:48Z |
| format | Article |
| id | doaj.art-a3f0166119db4492a0b92fde15130984 |
| institution | Directory Open Access Journal |
| issn | 1757-6512 |
| language | English |
| last_indexed | 2024-12-10T18:52:48Z |
| publishDate | 2022-08-01 |
| publisher | BMC |
| record_format | Article |
| series | Stem Cell Research & Therapy |
| spelling | doaj.art-a3f0166119db4492a0b92fde151309842022-12-22T01:37:16ZengBMCStem Cell Research & Therapy1757-65122022-08-0113111310.1186/s13287-022-03128-zBone marrow mesenchymal stromal cells in a 3D system produce higher concentration of extracellular vesicles (EVs) with increased complexity and enhanced neuronal growth propertiesElmira Jalilian0Hamed Massoumi1Bianca Bigit2Sohil Amin3Eitan A. Katz4Victor H. Guaiquil5Khandaker N. Anwar6Peiman Hematti7Mark I. Rosenblatt8Ali R. Djalilian9Department of Ophthalmology and Visual Sciences, Illinois Eye and Ear Infirmary, University of Illinois at ChicagoDepartment of Ophthalmology and Visual Sciences, Illinois Eye and Ear Infirmary, University of Illinois at ChicagoDepartment of Ophthalmology and Visual Sciences, Illinois Eye and Ear Infirmary, University of Illinois at ChicagoDepartment of Ophthalmology and Visual Sciences, Illinois Eye and Ear Infirmary, University of Illinois at ChicagoDepartment of Ophthalmology and Visual Sciences, Illinois Eye and Ear Infirmary, University of Illinois at ChicagoDepartment of Ophthalmology and Visual Sciences, Illinois Eye and Ear Infirmary, University of Illinois at ChicagoDepartment of Ophthalmology and Visual Sciences, Illinois Eye and Ear Infirmary, University of Illinois at ChicagoDepartment of Medicine, Hematology/Oncology Division, University of Wisconsin-Madison, School of Medicine and Public HealthDepartment of Ophthalmology and Visual Sciences, Illinois Eye and Ear Infirmary, University of Illinois at ChicagoDepartment of Ophthalmology and Visual Sciences, Illinois Eye and Ear Infirmary, University of Illinois at ChicagoHighlights Extracellular vesicles (EV) derived from 2D and 3D bone marrow mesenchymal stromal cells (BM-MSCs) demonstrate different characterization phenotype. Heterogeneity and concentration of BM-MSCs-derived extracellular vesicles are highly impacted by the culture conditions. 3D-derived EVs can significantly enhance trigeminal nerves elongation and complexity compared to 2D-derived EVs. 3D culture conditions are highly efficient environment for upscale production of MSC-derived EVs with enhanced neuro-regeneration properties for corneal nerve regeneration.https://doi.org/10.1186/s13287-022-03128-zExtracellular vesiclesExosomesBone marrow mesenchymal stromal cell3D culture2D cultureNeuronal growth |
| spellingShingle | Elmira Jalilian Hamed Massoumi Bianca Bigit Sohil Amin Eitan A. Katz Victor H. Guaiquil Khandaker N. Anwar Peiman Hematti Mark I. Rosenblatt Ali R. Djalilian Bone marrow mesenchymal stromal cells in a 3D system produce higher concentration of extracellular vesicles (EVs) with increased complexity and enhanced neuronal growth properties Stem Cell Research & Therapy Extracellular vesicles Exosomes Bone marrow mesenchymal stromal cell 3D culture 2D culture Neuronal growth |
| title | Bone marrow mesenchymal stromal cells in a 3D system produce higher concentration of extracellular vesicles (EVs) with increased complexity and enhanced neuronal growth properties |
| title_full | Bone marrow mesenchymal stromal cells in a 3D system produce higher concentration of extracellular vesicles (EVs) with increased complexity and enhanced neuronal growth properties |
| title_fullStr | Bone marrow mesenchymal stromal cells in a 3D system produce higher concentration of extracellular vesicles (EVs) with increased complexity and enhanced neuronal growth properties |
| title_full_unstemmed | Bone marrow mesenchymal stromal cells in a 3D system produce higher concentration of extracellular vesicles (EVs) with increased complexity and enhanced neuronal growth properties |
| title_short | Bone marrow mesenchymal stromal cells in a 3D system produce higher concentration of extracellular vesicles (EVs) with increased complexity and enhanced neuronal growth properties |
| title_sort | bone marrow mesenchymal stromal cells in a 3d system produce higher concentration of extracellular vesicles evs with increased complexity and enhanced neuronal growth properties |
| topic | Extracellular vesicles Exosomes Bone marrow mesenchymal stromal cell 3D culture 2D culture Neuronal growth |
| url | https://doi.org/10.1186/s13287-022-03128-z |
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