Heat Shock Protein Overexpression-Mediated Periodontal Ligament Regeneration: A Fundamental Approach to Generate a Potential Biomaterial
The periodontal ligament (PDL) is a cell-rich fibrous connective tissue supporting the tooth roots. The tissue helps to maintain homeostasis and exhibits regenerative and repairing ability, which is mediated by the heat shock protein (HSP). Here, we experimentally created PDL tissue with notable abi...
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| Format: | Article |
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MDPI AG
2022-01-01
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| Series: | Materials |
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| Online Access: | https://www.mdpi.com/1996-1944/15/3/809 |
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| author | Rina Muraoka Keisuke Nakano Toshiyuki Kawakami |
| author_facet | Rina Muraoka Keisuke Nakano Toshiyuki Kawakami |
| author_sort | Rina Muraoka |
| collection | DOAJ |
| description | The periodontal ligament (PDL) is a cell-rich fibrous connective tissue supporting the tooth roots. The tissue helps to maintain homeostasis and exhibits regenerative and repairing ability, which is mediated by the heat shock protein (HSP). Here, we experimentally created PDL tissue with notable ability to regenerate hard tissue and evaluated it as a potential biomaterial. We immunohistochemically examined the mechanical load-induced HSP overexpression in mouse PDL. Following mechanical load application and release, HSP70 localization in the PDL was altered immediately, suggesting that the HSP70 function may differ with the timing of its expression in PDL. HSP70 expressed in the cytoplasm and nucleus of fibroblasts in PDL on the tension side not only participated in periodontium repair, but also functioned as a molecular chaperone during protein expression involved in osteogenesis to restructure injured tissue. This study highlights the potential of artificially created highly functional PDL tissues as biomaterials. |
| first_indexed | 2024-03-09T23:38:13Z |
| format | Article |
| id | doaj.art-a3f2e500fdf246cea7c6cf7f80819d9c |
| institution | Directory Open Access Journal |
| issn | 1996-1944 |
| language | English |
| last_indexed | 2024-03-09T23:38:13Z |
| publishDate | 2022-01-01 |
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| record_format | Article |
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| spelling | doaj.art-a3f2e500fdf246cea7c6cf7f80819d9c2023-11-23T16:57:27ZengMDPI AGMaterials1996-19442022-01-0115380910.3390/ma15030809Heat Shock Protein Overexpression-Mediated Periodontal Ligament Regeneration: A Fundamental Approach to Generate a Potential BiomaterialRina Muraoka0Keisuke Nakano1Toshiyuki Kawakami2Department of Orthodontics, Matsumoto Dental University School of Dentistry and Hospital, 1780 Hirooka-Gobara, Shiojiri 399-0781, JapanDepartment of Oral Pathology and Medicine, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University, 2-5-1 Shikata-cho, Okayama 700-8525, JapanDepartment of Oral Pathology, School of Dentistry, Aichi Gakuin University, 1-100 Kusumoto-cho, Chikusa-ku, Nagoya 464-8650, JapanThe periodontal ligament (PDL) is a cell-rich fibrous connective tissue supporting the tooth roots. The tissue helps to maintain homeostasis and exhibits regenerative and repairing ability, which is mediated by the heat shock protein (HSP). Here, we experimentally created PDL tissue with notable ability to regenerate hard tissue and evaluated it as a potential biomaterial. We immunohistochemically examined the mechanical load-induced HSP overexpression in mouse PDL. Following mechanical load application and release, HSP70 localization in the PDL was altered immediately, suggesting that the HSP70 function may differ with the timing of its expression in PDL. HSP70 expressed in the cytoplasm and nucleus of fibroblasts in PDL on the tension side not only participated in periodontium repair, but also functioned as a molecular chaperone during protein expression involved in osteogenesis to restructure injured tissue. This study highlights the potential of artificially created highly functional PDL tissues as biomaterials.https://www.mdpi.com/1996-1944/15/3/809biomaterialsfibroblastsheat shock protein (HSP)homeostasismechanical loadperiodontal ligament (PDL) tissue |
| spellingShingle | Rina Muraoka Keisuke Nakano Toshiyuki Kawakami Heat Shock Protein Overexpression-Mediated Periodontal Ligament Regeneration: A Fundamental Approach to Generate a Potential Biomaterial Materials biomaterials fibroblasts heat shock protein (HSP) homeostasis mechanical load periodontal ligament (PDL) tissue |
| title | Heat Shock Protein Overexpression-Mediated Periodontal Ligament Regeneration: A Fundamental Approach to Generate a Potential Biomaterial |
| title_full | Heat Shock Protein Overexpression-Mediated Periodontal Ligament Regeneration: A Fundamental Approach to Generate a Potential Biomaterial |
| title_fullStr | Heat Shock Protein Overexpression-Mediated Periodontal Ligament Regeneration: A Fundamental Approach to Generate a Potential Biomaterial |
| title_full_unstemmed | Heat Shock Protein Overexpression-Mediated Periodontal Ligament Regeneration: A Fundamental Approach to Generate a Potential Biomaterial |
| title_short | Heat Shock Protein Overexpression-Mediated Periodontal Ligament Regeneration: A Fundamental Approach to Generate a Potential Biomaterial |
| title_sort | heat shock protein overexpression mediated periodontal ligament regeneration a fundamental approach to generate a potential biomaterial |
| topic | biomaterials fibroblasts heat shock protein (HSP) homeostasis mechanical load periodontal ligament (PDL) tissue |
| url | https://www.mdpi.com/1996-1944/15/3/809 |
| work_keys_str_mv | AT rinamuraoka heatshockproteinoverexpressionmediatedperiodontalligamentregenerationafundamentalapproachtogenerateapotentialbiomaterial AT keisukenakano heatshockproteinoverexpressionmediatedperiodontalligamentregenerationafundamentalapproachtogenerateapotentialbiomaterial AT toshiyukikawakami heatshockproteinoverexpressionmediatedperiodontalligamentregenerationafundamentalapproachtogenerateapotentialbiomaterial |